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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
lon and hupB are separated by 208 nucleotides
hupB and ppiD are separated by 191 nucleotides
ppiD and ybaV are separated by 150 nucleotides
ybaV and ybaW are separated by 93 nucleotides
b0439: lon - DNA-binding ATP-dependent protease La (NCBI), at 458,112 to 460,466
lon
b0440: hupB - HU, DNA-binding transcriptional regulator, beta subunit (NCBI), at 460,675 to 460,947
hupB
b0441: ppiD - peptidyl-prolyl cis-trans isomerase (rotamase D) (NCBI), at 461,139 to 463,010
ppiD
b0442: ybaV - hypothetical protein (NCBI), at 463,161 to 463,532
ybaV
b0443: ybaW - hypothetical protein (NCBI), at 463,626 to 464,024
ybaW
Group
Condition
b0439
b0440
b0441
b0442
b0443
lon
hupB
ppiD
ybaV
ybaW
motility
outer cut, LB soft agar motility assay
-3.9
-4.0
-0.8
+0.2
-0.1
carbon source
carbon source D-Glucose 0.25mM
-4.2
-1.0
+0.1
-1.7
+0.3
carbon source
carbon source D-Glucose 0.25mM
-4.2
-0.2
+0.1
-0.0
-1.8
carbon source
Ying_AminoAcid20 rep A; time point 3
-5.4
-0.4
+0.2
-0.2
-0.0
mzaabcde
mzaABCDE
-3.9
-1.4
-0.3
-0.1
+0.1
ppi
ppI
-4.3
-1.1
-0.2
+0.2
-0.1
carbon source
Ying_AminoAcid20 rep B; time point 3
-5.0
-0.7
+0.3
+0.2
-0.1
phage
Lambda_ci1857
-1.9
-3.4
+0.0
-0.4
+0.6
retron_ec_48_control
retron_Ec_48_control
-4.0
-1.0
-0.3
+0.3
-0.2
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.7
-0.2
-5.2
-0.1
-0.1
carbon source
Ying_Nucleobase14 rep B; time point 3
-2.2
-3.2
+0.1
+0.4
-0.1
sir2-hera
SIR2-HerA
-4.2
-0.9
-0.3
+0.2
+0.2
motility
outer cut, LB soft agar motility assay
-4.2
-0.7
-0.6
+0.3
+0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.6
+1.3
-4.9
-0.1
-1.0
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+3.7
-3.5
-3.9
-0.2
-0.0
stress
octanol 0.08 vol%
+5.1
-3.8
-4.4
-0.6
-0.3
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+1.0
+0.7
-5.2
-0.1
-0.5
micoeukaryotes
Tetrahymena sp., Mixed culture-1
-3.5
-1.0
-0.4
+0.1
+0.9
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+1.2
+0.8
-5.6
-0.1
+0.1
phage
Δdbn_10
-3.2
+1.2
-0.0
-0.4
-1.0
stress
Benzalkonium Chloride 0.004 mg/ml
+4.2
-3.6
-3.6
+0.2
+0.4
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.7
+1.5
-3.6
-0.1
+0.3
stress
LB Aerobic with Spiramycin 0.000125 mM
+4.3
-1.9
-0.2
-0.2
+0.1
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
+4.1
-1.2
-0.3
-0.1
+0.4
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
+4.6
-0.8
-0.3
-0.1
+0.4
stress
Doxycycline hyclate 0.001 mg/ml
+5.7
-1.2
+0.0
-0.2
+0.0
stress
Chloramphenicol 0.0025 mg/ml
+5.2
-1.4
+0.2
+0.0
+0.2
stress
LB Aerobic with Doxycycline hyclate 0.0000015625 mM
+5.8
-0.9
+0.4
+0.3
-0.2
stress
LB Aerobic with Doxycycline hyclate 0.0000015625 mM
+5.9
-0.4
+0.4
+0.1
-0.2
stress
Cephalothin 0.01 mg/ml
+3.5
+2.0
+1.4
-0.7
+0.2
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