Fitness for 5 genes in Escherichia coli BW25113

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500 ntlon and hupB are separated by 208 nucleotideshupB and ppiD are separated by 191 nucleotidesppiD and ybaV are separated by 150 nucleotidesybaV and ybaW are separated by 93 nucleotides b0439: lon - DNA-binding ATP-dependent protease La (NCBI), at 458,112 to 460,466 lon b0440: hupB - HU, DNA-binding transcriptional regulator, beta subunit (NCBI), at 460,675 to 460,947 hupB b0441: ppiD - peptidyl-prolyl cis-trans isomerase (rotamase D) (NCBI), at 461,139 to 463,010 ppiD b0442: ybaV - hypothetical protein (NCBI), at 463,161 to 463,532 ybaV b0443: ybaW - hypothetical protein (NCBI), at 463,626 to 464,024 ybaW
Group Condition b0439 b0440 b0441 b0442 b0443
lon hupB ppiD ybaV ybaW
motility outer cut, LB soft agar motility assay -3.9 -4.0 -0.8 +0.2 -0.1
carbon source carbon source D-Glucose 0.25mM -4.2 -1.0 +0.1 -1.7 +0.3
carbon source carbon source D-Glucose 0.25mM -4.2 -0.2 +0.1 -0.0 -1.8
carbon source Ying_AminoAcid20 rep A; time point 3 -5.4 -0.4 +0.2 -0.2 -0.0
mzaabcde mzaABCDE -3.9 -1.4 -0.3 -0.1 +0.1
ppi ppI -4.3 -1.1 -0.2 +0.2 -0.1
carbon source Ying_AminoAcid20 rep B; time point 3 -5.0 -0.7 +0.3 +0.2 -0.1
phage Lambda_ci1857 -1.9 -3.4 +0.0 -0.4 +0.6
retron_ec_48_control retron_Ec_48_control -4.0 -1.0 -0.3 +0.3 -0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.7 -0.2 -5.2 -0.1 -0.1
carbon source Ying_Nucleobase14 rep B; time point 3 -2.2 -3.2 +0.1 +0.4 -0.1
sir2-hera SIR2-HerA -4.2 -0.9 -0.3 +0.2 +0.2
motility outer cut, LB soft agar motility assay -4.2 -0.7 -0.6 +0.3 +0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.6 +1.3 -4.9 -0.1 -1.0
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +3.7 -3.5 -3.9 -0.2 -0.0
stress octanol 0.08 vol% +5.1 -3.8 -4.4 -0.6 -0.3
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.0 +0.7 -5.2 -0.1 -0.5
micoeukaryotes Tetrahymena sp., Mixed culture-1 -3.5 -1.0 -0.4 +0.1 +0.9
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.2 +0.8 -5.6 -0.1 +0.1
phage Δdbn_10 -3.2 +1.2 -0.0 -0.4 -1.0
stress Benzalkonium Chloride 0.004 mg/ml +4.2 -3.6 -3.6 +0.2 +0.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.7 +1.5 -3.6 -0.1 +0.3
stress LB Aerobic with Spiramycin 0.000125 mM +4.3 -1.9 -0.2 -0.2 +0.1
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM +4.1 -1.2 -0.3 -0.1 +0.4
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM +4.6 -0.8 -0.3 -0.1 +0.4
stress Doxycycline hyclate 0.001 mg/ml +5.7 -1.2 +0.0 -0.2 +0.0
stress Chloramphenicol 0.0025 mg/ml +5.2 -1.4 +0.2 +0.0 +0.2
stress LB Aerobic with Doxycycline hyclate 0.0000015625 mM +5.8 -0.9 +0.4 +0.3 -0.2
stress LB Aerobic with Doxycycline hyclate 0.0000015625 mM +5.9 -0.4 +0.4 +0.1 -0.2
stress Cephalothin 0.01 mg/ml +3.5 +2.0 +1.4 -0.7 +0.2
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