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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
cyoB and cyoA are separated by 21 nucleotides
cyoA and ampG are separated by 459 nucleotides
ampG and yajG are separated by 43 nucleotides
yajG and bolA are separated by 304 nucleotides
b0431: cyoB - cytochrome o ubiquinol oxidase subunit I (NCBI), at 447,874 to 449,865
cyoB
b0432: cyoA - cytochrome o ubiquinol oxidase subunit II (NCBI), at 449,887 to 450,834
cyoA
b0433: ampG - muropeptide transporter (NCBI), at 451,294 to 452,769
ampG
b0434: yajG - putative polymerase/proteinase (VIMSS), at 452,813 to 453,391
yajG
b0435: bolA - possible regulator of murein genes (VIMSS), at 453,696 to 454,013
bolA
Group
Condition
b0431
b0432
b0433
b0434
b0435
cyoB
cyoA
ampG
yajG
bolA
stress
undecene 5 vol%
-5.1
-4.7
+0.1
+0.1
+0.2
stress
2,4-Dinitrophenol 2 mM
-4.9
-4.0
-0.3
-0.1
-0.0
stress
undecene 5 vol%
-5.0
-4.6
+0.1
+0.2
+0.1
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.2
-0.5
-5.2
-4.2
+0.9
stress
2,4-Dinitrophenol 2 mM
-4.2
-3.7
-0.3
+0.1
-0.2
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.1
-0.6
-4.0
-4.2
+0.6
stress
Carbenicillin 0.008 mg/ml
-1.7
-1.0
-2.8
-2.4
+0.1
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.2
-0.8
-3.5
-3.2
+0.4
stress
Carbenicillin 0.004 mg/ml
-1.6
-0.8
-2.9
-1.6
-0.0
stress
LB Anaerobic with Ticarcillin 0.00003125 mM
+0.1
+0.1
-4.6
-2.6
+0.3
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+0.3
-0.2
-4.5
-2.6
+0.4
stress
hexanol 0.31 vol%
-3.8
-2.8
+0.3
-0.3
-0.0
stress
LB Aerobic with Irgasan 0.0000003125 mM
-2.4
-1.9
-0.4
-2.5
+0.7
stress
LB Anaerobic with Penicillin g 0.0000625 mM
+0.1
-0.1
-4.8
-1.7
+0.0
stress
LB Anaerobic with Azlocillin 0.00003125 mM
+0.0
-0.0
-4.4
-2.2
+0.2
stress
LB Anaerobic with Ticarcillin 0.00003125 mM
-0.0
-0.1
-4.4
-2.2
+0.6
stress
Cephalothin 0.01 mg/ml
-0.9
+0.3
-3.2
-2.7
+0.4
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
+0.1
-0.0
-3.7
-2.6
+0.3
stress
3-methyl-2-butenol 1.25 vol%
-3.3
-3.1
+0.0
+0.2
+0.3
stress
3-methyl-2-butenol 1.25 vol%
-3.7
-2.6
-0.1
+0.1
+0.4
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+2.7
+3.2
+0.1
-0.2
+0.4
plasmid transformation
vcDART vector transformation; agar assay
+3.1
+3.4
+0.1
+0.0
+0.3
stress
Cisplatin 0.1 mg/ml
+3.4
+4.3
+0.0
+0.0
-0.4
plasmid transformation
vcDART vector transformation; agar assay
+3.5
+4.0
+0.1
+0.0
+0.0
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+3.8
+4.4
-0.4
+0.1
-0.2
stress
Spectinomycin 0.0125 mg/ml
+3.9
+4.3
+0.1
-0.1
+0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+4.0
+4.3
-0.1
+0.0
+0.3
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+4.2
+4.9
-0.1
-0.2
-0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+4.9
+5.5
-0.0
+0.2
+0.0
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+6.1
+7.1
-0.1
-0.3
+0.4
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