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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
cyoD and cyoC overlap by 1 nucleotides
cyoC and cyoB overlap by 11 nucleotides
cyoB and cyoA are separated by 21 nucleotides
cyoA and ampG are separated by 459 nucleotides
b0429: cyoD - cytochrome o ubiquinol oxidase subunit IV (NCBI), at 446,941 to 447,270
cyoD
b0430: cyoC - cytochrome o ubiquinol oxidase subunit III (NCBI), at 447,270 to 447,884
cyoC
b0431: cyoB - cytochrome o ubiquinol oxidase subunit I (NCBI), at 447,874 to 449,865
cyoB
b0432: cyoA - cytochrome o ubiquinol oxidase subunit II (NCBI), at 449,887 to 450,834
cyoA
b0433: ampG - muropeptide transporter (NCBI), at 451,294 to 452,769
ampG
Group
Condition
b0429
b0430
b0431
b0432
b0433
cyoD
cyoC
cyoB
cyoA
ampG
stress
2,4-Dinitrophenol 2 mM
-6.9
-4.9
-4.9
-4.0
-0.3
stress
undecene 5 vol%
-4.9
-4.5
-5.1
-4.7
+0.1
stress
undecene 5 vol%
-4.3
-4.5
-5.0
-4.6
+0.1
stress
2,4-Dinitrophenol 2 mM
-3.9
-4.2
-4.2
-3.7
-0.3
stress
hexanol 0.31 vol%
-2.9
-4.2
-3.8
-2.8
+0.3
stress
3-methyl-2-butenol 1.25 vol%
-2.6
-4.3
-3.3
-3.1
+0.0
stress
3-methyl-2-butenol 1.25 vol%
-2.4
-3.4
-3.7
-2.6
-0.1
carbon source
Glycolic Acid (C)
-2.3
-3.5
-2.8
-2.7
+0.1
stress
Vanillin 5 mM
-2.8
-2.8
-2.9
-2.4
-0.1
stress
ethyl acetate 5 vol%
-2.9
-3.4
-2.6
-2.2
+0.0
carbon source
succinate (C)
-2.7
-3.2
-2.5
-3.0
+0.4
stress
undecene 2.5 vol%
-3.0
-2.5
-2.6
-2.7
+0.2
carbon source
Glycolic Acid (C)
-2.4
-2.5
-2.7
-2.9
+0.4
carbon source
succinate (C)
-2.2
-2.4
-2.9
-2.8
+0.4
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+2.4
+2.3
+2.5
+3.0
+0.3
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+2.3
+2.3
+2.7
+3.2
+0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+3.2
+2.8
+2.5
+3.2
-0.1
plasmid transformation
vcDART vector transformation; agar assay
+3.1
+3.4
+2.9
+3.2
+0.1
plasmid transformation
mariner vector transformation; agar assay
+3.4
+3.4
+2.8
+3.4
+0.0
plasmid transformation
mariner vector transformation; agar assay
+3.4
+3.5
+2.9
+3.4
-0.0
plasmid transformation
mariner vector transformation; agar assay
+3.5
+3.7
+3.0
+3.3
+0.0
plasmid transformation
vcDART vector transformation; agar assay
+3.5
+3.6
+3.1
+3.4
+0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+4.1
+3.5
+3.8
+4.4
-0.4
plasmid transformation
vcDART vector transformation; agar assay
+4.0
+4.1
+3.5
+4.0
+0.1
stress
Cisplatin 0.1 mg/ml
+4.2
+3.7
+3.4
+4.3
+0.0
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+4.0
+3.6
+4.0
+4.3
-0.1
stress
Spectinomycin 0.0125 mg/ml
+4.0
+3.8
+3.9
+4.3
+0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+4.8
+4.1
+4.2
+4.9
-0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+5.4
+4.8
+4.9
+5.5
-0.0
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+7.0
+6.5
+6.1
+7.1
-0.1
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