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Protein
Homologs
Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
thiJ and panE overlap by 38 nucleotides
panE and yajQ are separated by 167 nucleotides
yajQ and yajR are separated by 127 nucleotides
yajR and cyoE are separated by 148 nucleotides
b0424: thiJ - 4-methyl-5(beta-hydroxyethyl)-thiazole monophosphate synthesis (VIMSS), at 442,275 to 442,865
thiJ
b0425: panE - 2-dehydropantoate 2-reductase (NCBI), at 442,828 to 443,739
panE
b0426: yajQ - orf, hypothetical protein (VIMSS), at 443,907 to 444,398
yajQ
b0427: yajR - putative transport protein (VIMSS), at 444,526 to 445,890
yajR
b0428: cyoE - protoheme IX farnesyltransferase (NCBI), at 446,039 to 446,929
cyoE
Group
Condition
b0424
b0425
b0426
b0427
b0428
thiJ
panE
yajQ
yajR
cyoE
stress
2,4-Dinitrophenol 2 mM
-0.1
-0.6
-0.7
-3.2
-5.5
stress
undecene 5 vol%
-0.1
+0.2
-0.3
-4.9
-4.9
stress
undecene 5 vol%
+0.1
+0.3
-0.3
-4.9
-4.5
stress
2,4-Dinitrophenol 2 mM
-0.0
-0.5
-0.1
-2.7
-4.2
stress
undecene 2.5 vol%
+0.2
+0.2
-0.2
-2.4
-3.6
stress
undecene 2.5 vol%
+0.1
+0.1
-0.2
-2.2
-2.6
stress
ethyl acetate 5 vol%
+0.1
-0.1
+0.3
-1.7
-3.1
carbon source
casaminos (C)
+0.1
-0.7
+0.3
-1.1
-2.8
stress
3-methyl-2-butenol 1.25 vol%
-0.2
+0.1
+0.3
-0.1
-4.2
carbon source
casaminos (C)
-0.1
-0.6
+0.3
-1.1
-2.6
carbon source
D-Ribose (C)
+0.3
-0.5
+0.0
-1.1
-2.8
carbon source
L-Malic (C)
+0.2
-0.3
+0.3
-1.1
-2.9
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
-0.2
+0.6
+0.1
-1.9
-2.1
carbon source
Glycolic Acid (C)
+0.2
+0.6
+1.1
-1.3
-3.6
carbon source
Glycolic Acid (C)
-0.0
+0.7
+1.1
-0.9
-2.8
stress
2-Furfuraldehyde 0.25 vol%
-0.1
-0.3
+0.4
+1.6
+2.5
plasmid transformation
mariner vector transformation; agar assay
+0.3
-0.1
+0.1
+2.1
+3.4
plasmid transformation
mariner vector transformation; agar assay
+0.2
+0.1
-0.1
+2.3
+3.5
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.2
-0.0
+0.6
+2.1
+3.2
plasmid transformation
mariner vector transformation; agar assay
+0.1
-0.0
+0.1
+2.4
+3.6
stress
Cisplatin 0.1 mg/ml
-0.1
-0.2
+0.3
+2.0
+4.4
plasmid transformation
vcDART vector transformation; agar assay
+0.3
+0.1
+0.3
+2.7
+3.3
plasmid transformation
vcDART vector transformation; agar assay
+0.1
+0.2
+0.4
+2.7
+3.4
stress
Spectinomycin 0.0125 mg/ml
+0.5
+0.0
+0.1
+1.9
+4.3
plasmid transformation
vcDART vector transformation; agar assay
+0.2
+0.1
+0.2
+3.1
+4.0
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.2
+0.1
+0.7
+2.9
+4.3
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.5
-0.0
+0.7
+2.9
+4.6
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.1
-0.1
+1.0
+3.6
+5.0
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.0
+0.0
+0.6
+3.9
+5.6
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.2
+0.0
+1.1
+4.2
+7.4
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