Fitness for 5 genes in Escherichia coli BW25113

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500 ntthiJ and panE overlap by 38 nucleotidespanE and yajQ are separated by 167 nucleotidesyajQ and yajR are separated by 127 nucleotidesyajR and cyoE are separated by 148 nucleotides b0424: thiJ - 4-methyl-5(beta-hydroxyethyl)-thiazole monophosphate synthesis (VIMSS), at 442,275 to 442,865 thiJ b0425: panE - 2-dehydropantoate 2-reductase (NCBI), at 442,828 to 443,739 panE b0426: yajQ - orf, hypothetical protein (VIMSS), at 443,907 to 444,398 yajQ b0427: yajR - putative transport protein (VIMSS), at 444,526 to 445,890 yajR b0428: cyoE - protoheme IX farnesyltransferase (NCBI), at 446,039 to 446,929 cyoE
Group Condition b0424 b0425 b0426 b0427 b0428
thiJ panE yajQ yajR cyoE
stress 2,4-Dinitrophenol 2 mM -0.1 -0.6 -0.7 -3.2 -5.5
stress undecene 5 vol% -0.1 +0.2 -0.3 -4.9 -4.9
stress undecene 5 vol% +0.1 +0.3 -0.3 -4.9 -4.5
stress 2,4-Dinitrophenol 2 mM -0.0 -0.5 -0.1 -2.7 -4.2
stress undecene 2.5 vol% +0.2 +0.2 -0.2 -2.4 -3.6
stress undecene 2.5 vol% +0.1 +0.1 -0.2 -2.2 -2.6
stress ethyl acetate 5 vol% +0.1 -0.1 +0.3 -1.7 -3.1
carbon source casaminos (C) +0.1 -0.7 +0.3 -1.1 -2.8
stress 3-methyl-2-butenol 1.25 vol% -0.2 +0.1 +0.3 -0.1 -4.2
carbon source casaminos (C) -0.1 -0.6 +0.3 -1.1 -2.6
carbon source D-Ribose (C) +0.3 -0.5 +0.0 -1.1 -2.8
carbon source L-Malic (C) +0.2 -0.3 +0.3 -1.1 -2.9
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -0.2 +0.6 +0.1 -1.9 -2.1
carbon source Glycolic Acid (C) +0.2 +0.6 +1.1 -1.3 -3.6
carbon source Glycolic Acid (C) -0.0 +0.7 +1.1 -0.9 -2.8
stress 2-Furfuraldehyde 0.25 vol% -0.1 -0.3 +0.4 +1.6 +2.5
plasmid transformation mariner vector transformation; agar assay +0.3 -0.1 +0.1 +2.1 +3.4
plasmid transformation mariner vector transformation; agar assay +0.2 +0.1 -0.1 +2.3 +3.5
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.2 -0.0 +0.6 +2.1 +3.2
plasmid transformation mariner vector transformation; agar assay +0.1 -0.0 +0.1 +2.4 +3.6
stress Cisplatin 0.1 mg/ml -0.1 -0.2 +0.3 +2.0 +4.4
plasmid transformation vcDART vector transformation; agar assay +0.3 +0.1 +0.3 +2.7 +3.3
plasmid transformation vcDART vector transformation; agar assay +0.1 +0.2 +0.4 +2.7 +3.4
stress Spectinomycin 0.0125 mg/ml +0.5 +0.0 +0.1 +1.9 +4.3
plasmid transformation vcDART vector transformation; agar assay +0.2 +0.1 +0.2 +3.1 +4.0
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.2 +0.1 +0.7 +2.9 +4.3
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.5 -0.0 +0.7 +2.9 +4.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.1 -0.1 +1.0 +3.6 +5.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.0 +0.0 +0.6 +3.9 +5.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.2 +0.0 +1.1 +4.2 +7.4
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