Fitness for 5 genes in Escherichia coli BW25113

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500 ntsecF and yajD are separated by 128 nucleotidesyajD and tsx are separated by 176 nucleotidestsx and yajI are separated by 298 nucleotidesyajI and ybaD are separated by 150 nucleotides b0409: secF - protein export protein SecF (NCBI), at 428,729 to 429,700 secF b0410: yajD - hypothetical protein (NCBI), at 429,829 to 430,176 yajD b0411: tsx - nucleoside channel, receptor of phage T6 and colicin K (NCBI), at 430,353 to 431,237 tsx b0412: yajI - orf, hypothetical protein (VIMSS), at 431,536 to 432,075 yajI b0413: ybaD - hypothetical protein (NCBI), at 432,226 to 432,675 ybaD
Group Condition b0409 b0410 b0411 b0412 b0413
secF yajD tsx yajI ybaD
stress octanol 0.08 vol% N.D. +0.3 -1.7 -1.8 N.D.
stress Trimethoprim 0.005 mM N.D. -0.5 -1.8 -0.1 N.D.
phage N4_phage 0.009375 MOI TC2 N.D. -0.2 -0.8 -0.6 N.D.
stress Cephalothin 0.01 mg/ml N.D. -0.8 -0.8 +0.1 N.D.
carbon source carbon source D-Mannose 0.5mM N.D. -1.2 -0.0 -0.2 N.D.
stress Trimethoprim 0.0025 mM N.D. -0.5 -1.0 +0.1 N.D.
stress LB Anaerobic with Ampicillin 0.00000195313 mM N.D. -0.2 -0.6 -0.6 N.D.
stress LB Anaerobic with Cephalothin 0.0000078125 mM N.D. +0.2 -1.2 -0.3 N.D.
stress Ciprofloxacin Hydrochloride 0.0003 mM N.D. -0.0 -0.3 -1.1 N.D.
stress Trimethoprim 0.00125 mM N.D. -0.3 -0.9 -0.1 N.D.
phage Ste41 N.D. -0.1 -0.8 -0.4 N.D.
phage Δdbn_10 N.D. -0.6 -0.5 -0.2 N.D.
phage Lambda_ci1857 N.D. -0.6 -0.6 -0.1 N.D.
control PBS_cage_1_mouse_1_Cecum_40 hr N.D. -0.8 -0.5 +0.1 N.D.
phage N4_phage 0.00009375 MOI TC4 N.D. +0.1 -0.6 -0.7 N.D.
stress Ciprofloxacin Hydrochloride 0.0024 mM N.D. -0.5 -0.8 +0.2 N.D.
carbon source carbon source D-Mannose 1mM N.D. +0.1 -0.2 -0.9 N.D.
stress LB Anaerobic with Cephalothin 0.0000078125 mM N.D. -0.2 -1.1 +0.5 N.D.
phage T7_phage_31.25_MOI N.D. +0.1 -0.8 +0.5 N.D.
phage Ste41 N.D. -0.4 -0.3 +0.5 N.D.
stress 1-ethyl-3-methylimidazolium chloride 40 mM N.D. +0.2 -0.9 +0.5 N.D.
stress LB Aerobic with Nitrofurantoin 0.00003125 mM N.D. +0.1 +0.7 -0.8 N.D.
phage T7_phage 0.00015625 MOI TC4 N.D. -0.6 +0.7 -0.0 N.D.
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. +0.3 -0.5 +0.8 N.D.
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr N.D. +0.7 -0.3 +0.4 N.D.
stress 2,4-Dinitrophenol 2 mM N.D. +0.1 +1.0 -0.2 N.D.
stress 2,4-Dinitrophenol 2 mM N.D. -0.0 +1.4 -0.4 N.D.
stress Spectinomycin 0.0125 mg/ml N.D. -0.1 +0.9 +0.5 N.D.
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM N.D. +0.7 +0.2 +0.5 N.D.
phage NoPhageControl_ML9a N.D. -0.2 +4.5 -0.2 N.D.
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