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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
ykiA and rdgC are separated by 157 nucleotides
rdgC and yajF are separated by 124 nucleotides
yajF and araJ are separated by 244 nucleotides
araJ and sbcC are separated by 125 nucleotides
b0392: ykiA - hypothetical protein (NCBI), at 407,833 to 408,174
ykiA
b0393: rdgC - recombination associated protein (NCBI), at 408,332 to 409,243
rdgC
b0394: yajF - possible NAGC-like transcriptional regulator (VIMSS), at 409,368 to 410,276
yajF
b0396: araJ - involved in either transport or processing of arabinose polymers (VIMSS), at 410,521 to 411,705
araJ
b0397: sbcC - exonuclease, dsDNA, ATP-dependent (NCBI), at 411,831 to 414,977
sbcC
Group
Condition
b0392
b0393
b0394
b0396
b0397
ykiA
rdgC
yajF
araJ
sbcC
phage
Lambda_ci1857
-0.0
-4.1
+0.0
-0.2
-0.2
motility
outer cut, LB soft agar motility assay
-0.1
-0.9
-0.4
-0.3
+0.1
stress
Bacitracin 1 mg/ml
+0.0
-1.5
+0.2
-0.1
-0.0
stress
LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM
+0.3
+0.5
-0.1
-0.2
-0.7
stress
Ciprofloxacin Hydrochloride 0.0003 mM
-0.1
-0.6
-0.3
-0.1
+1.0
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.6
+1.1
-0.6
-0.1
+0.4
stress
Ciprofloxacin Hydrochloride 0.0003 mM
+0.2
-0.3
-0.3
-0.2
+1.0
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.3
+1.3
-0.5
-0.2
+0.3
stress
Ciprofloxacin Hydrochloride 0.0024 mM
-0.2
-0.4
+0.3
-0.1
+1.0
phage
Ste41
-0.4
+0.6
-0.4
-0.1
+0.9
stress
LB Anaerobic with Cefoxitin 0.000003125 mM
+0.1
+1.1
-0.3
-0.1
-0.1
stress
LB Anaerobic with Furaltadone 0.000015625 mM
+0.3
+0.9
-0.3
-0.1
+0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.3
+1.3
-0.4
-0.2
+0.5
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.7
+1.2
+0.3
-0.3
+0.5
phage
RB68
-0.2
+0.7
-0.1
-0.1
+0.8
phage
RB68
-0.5
+0.6
+0.0
+0.2
+0.8
phage
T7_phage 0.00015625 MOI TC3
+0.3
+0.7
-0.2
-0.0
+0.6
phage
T7_phage 0.0015625 MOI TC2
+0.2
+0.4
-0.4
+0.3
+0.8
stress
LB Aerobic with Irgasan 0.0000003125 mM
+0.1
+1.3
+0.2
+0.1
-0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.0
+0.9
-0.2
+0.3
+0.7
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
-0.0
+1.4
+0.1
+0.2
+0.1
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
-0.0
+1.5
+0.3
-0.2
+0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.3
+1.7
-0.0
-0.2
+0.6
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
+0.0
+1.9
-0.1
+0.1
-0.1
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
+0.0
+1.6
-0.1
+0.1
+0.2
stress
Carbenicillin 0.008 mg/ml
+0.2
+1.3
+0.0
+0.0
+0.3
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.0
+2.6
-0.3
-0.2
-0.1
stress
Cephalothin 0.01 mg/ml
+0.0
+2.3
-0.0
+0.0
-0.2
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
+0.2
+1.8
+0.0
+0.2
+0.0
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+0.1
+2.9
-0.7
-0.0
+0.0
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