Fitness for 5 genes in Escherichia coli BW25113

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500 ntykiA and rdgC are separated by 157 nucleotidesrdgC and yajF are separated by 124 nucleotidesyajF and araJ are separated by 244 nucleotidesaraJ and sbcC are separated by 125 nucleotides b0392: ykiA - hypothetical protein (NCBI), at 407,833 to 408,174 ykiA b0393: rdgC - recombination associated protein (NCBI), at 408,332 to 409,243 rdgC b0394: yajF - possible NAGC-like transcriptional regulator (VIMSS), at 409,368 to 410,276 yajF b0396: araJ - involved in either transport or processing of arabinose polymers (VIMSS), at 410,521 to 411,705 araJ b0397: sbcC - exonuclease, dsDNA, ATP-dependent (NCBI), at 411,831 to 414,977 sbcC
Group Condition b0392 b0393 b0394 b0396 b0397
ykiA rdgC yajF araJ sbcC
phage Lambda_ci1857 -0.0 -4.1 +0.0 -0.2 -0.2
motility outer cut, LB soft agar motility assay -0.1 -0.9 -0.4 -0.3 +0.1
stress Bacitracin 1 mg/ml +0.0 -1.5 +0.2 -0.1 -0.0
stress LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM +0.3 +0.5 -0.1 -0.2 -0.7
stress Ciprofloxacin Hydrochloride 0.0003 mM -0.1 -0.6 -0.3 -0.1 +1.0
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.6 +1.1 -0.6 -0.1 +0.4
stress Ciprofloxacin Hydrochloride 0.0003 mM +0.2 -0.3 -0.3 -0.2 +1.0
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.3 +1.3 -0.5 -0.2 +0.3
stress Ciprofloxacin Hydrochloride 0.0024 mM -0.2 -0.4 +0.3 -0.1 +1.0
phage Ste41 -0.4 +0.6 -0.4 -0.1 +0.9
stress LB Anaerobic with Cefoxitin 0.000003125 mM +0.1 +1.1 -0.3 -0.1 -0.1
stress LB Anaerobic with Furaltadone 0.000015625 mM +0.3 +0.9 -0.3 -0.1 +0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.3 +1.3 -0.4 -0.2 +0.5
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.7 +1.2 +0.3 -0.3 +0.5
phage RB68 -0.2 +0.7 -0.1 -0.1 +0.8
phage RB68 -0.5 +0.6 +0.0 +0.2 +0.8
phage T7_phage 0.00015625 MOI TC3 +0.3 +0.7 -0.2 -0.0 +0.6
phage T7_phage 0.0015625 MOI TC2 +0.2 +0.4 -0.4 +0.3 +0.8
stress LB Aerobic with Irgasan 0.0000003125 mM +0.1 +1.3 +0.2 +0.1 -0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.0 +0.9 -0.2 +0.3 +0.7
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM -0.0 +1.4 +0.1 +0.2 +0.1
stress LB Anaerobic with Amoxicillin 0.0000078125 mM -0.0 +1.5 +0.3 -0.2 +0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.3 +1.7 -0.0 -0.2 +0.6
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM +0.0 +1.9 -0.1 +0.1 -0.1
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM +0.0 +1.6 -0.1 +0.1 +0.2
stress Carbenicillin 0.008 mg/ml +0.2 +1.3 +0.0 +0.0 +0.3
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.0 +2.6 -0.3 -0.2 -0.1
stress Cephalothin 0.01 mg/ml +0.0 +2.3 -0.0 +0.0 -0.2
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM +0.2 +1.8 +0.0 +0.2 +0.0
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.1 +2.9 -0.7 -0.0 +0.0
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