Fitness for 5 genes in Escherichia coli BW25113

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500 ntyaiE and ykiA are separated by 147 nucleotidesykiA and rdgC are separated by 157 nucleotidesrdgC and yajF are separated by 124 nucleotidesyajF and araJ are separated by 244 nucleotides b0391: yaiE - hypothetical protein (NCBI), at 407,401 to 407,685 yaiE b0392: ykiA - hypothetical protein (NCBI), at 407,833 to 408,174 ykiA b0393: rdgC - recombination associated protein (NCBI), at 408,332 to 409,243 rdgC b0394: yajF - possible NAGC-like transcriptional regulator (VIMSS), at 409,368 to 410,276 yajF b0396: araJ - involved in either transport or processing of arabinose polymers (VIMSS), at 410,521 to 411,705 araJ
Group Condition b0391 b0392 b0393 b0394 b0396
yaiE ykiA rdgC yajF araJ
phage Lambda_ci1857 -0.6 -0.0 -4.1 +0.0 -0.2
motility outer cut, LB soft agar motility assay -0.5 -0.1 -0.9 -0.4 -0.3
stress Bacitracin 1 mg/ml +0.0 +0.0 -1.5 +0.2 -0.1
phage Ste41 -0.7 -0.4 +0.6 -0.4 -0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.0 -0.6 +1.1 -0.6 -0.1
nophagecontrol R1_N4_T1_phage0 -0.2 +0.4 +0.3 -0.6 -0.0
phage RB68 -0.4 -0.5 +0.6 +0.0 +0.2
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr +0.6 -0.5 -0.4 +0.1 +0.1
phage Δdbn_10 -0.5 -0.2 +0.5 +0.3 -0.1
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.2 -0.3 +0.6 -0.2 +0.3
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.0 -0.3 +1.3 -0.5 -0.2
stress LB Anaerobic with Furaltadone 0.000015625 mM -0.3 +0.3 +0.9 -0.3 -0.1
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr +0.4 +0.2 -0.5 +0.0 +0.4
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.0 -0.3 +1.3 -0.4 -0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.4 -0.7 +1.2 +0.3 -0.3
stress LB Anaerobic with Cefoxitin 0.000003125 mM +0.2 +0.1 +1.1 -0.3 -0.1
stress LB Anaerobic with Amoxicillin 0.0000078125 mM +0.2 -0.1 +1.3 -0.2 +0.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.1 -0.3 +1.7 -0.0 -0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.4 +0.0 +0.9 -0.2 +0.3
stress Carbenicillin 0.008 mg/ml -0.1 +0.2 +1.3 +0.0 +0.0
stress 1-ethyl-3-methylimidazolium chloride 40 mM +0.1 +0.1 +1.0 +0.4 -0.0
stress LB Anaerobic with Amoxicillin 0.0000078125 mM +0.1 -0.0 +1.5 +0.3 -0.2
stress LB Aerobic with Irgasan 0.0000003125 mM +0.0 +0.1 +1.3 +0.2 +0.1
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM +0.1 -0.0 +1.4 +0.1 +0.2
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM +0.2 +0.0 +1.6 -0.1 +0.1
stress Cephalothin 0.01 mg/ml -0.4 +0.0 +2.3 -0.0 +0.0
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.3 +0.1 +2.9 -0.7 -0.0
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.1 -0.0 +2.6 -0.3 -0.2
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM +0.3 +0.0 +1.9 -0.1 +0.1
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM +0.3 +0.2 +1.8 +0.0 +0.2
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