Fitness for 5 genes in Escherichia coli BW25113

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500 ntyaiV and ampH are separated by 0 nucleotidesampH and sbmA are separated by 351 nucleotidessbmA and yaiW are separated by 12 nucleotidesyaiW and yaiY are separated by 58 nucleotides b0375: yaiV - predicted DNA-binding transcriptional regulator (RefSeq), at 393,730 to 394,353 yaiV b0376: ampH - beta-lactamase/D-alanine carboxypeptidase (NCBI), at 394,354 to 395,511 ampH b0377: sbmA - predicted transporter (NCBI), at 395,863 to 397,083 sbmA b0378: yaiW - predicted DNA-binding transcriptional regulator (NCBI), at 397,096 to 398,190 yaiW b0379: yaiY - predicted inner membrane protein (NCBI), at 398,249 to 398,557 yaiY
Group Condition b0375 b0376 b0377 b0378 b0379
yaiV ampH sbmA yaiW yaiY
stress 2,4-Dinitrophenol 2 mM +0.0 -0.0 -3.2 +0.2 -0.2
carbon source carbon source D-Mannose 5mM -0.6 -0.4 -0.2 -0.5 -1.4
phage T7_phage 0.00015625 MOI TC4 +0.4 -0.2 -0.3 -0.4 -2.2
stress 2,4-Dinitrophenol 2 mM +0.0 +0.1 -2.8 +0.0 -0.0
stress LB Aerobic with Ramoplanin 0.000125 mM -0.8 -1.1 +0.4 +0.1 -0.1
control PBS_cage_1_mouse_1_Cecum_40 hr -1.3 +0.2 +0.0 -0.2 +0.0
mzaabcde mzaABCDE -0.6 +0.2 +0.1 +0.0 -0.9
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.5 -0.4 -0.3 -0.5 -0.3
phage T7_phage 0.0015625 MOI TC1 +0.5 +0.0 -0.6 -0.2 -0.5
phage T7_phage 0.00015625 MOI TC3 +0.5 +0.1 -0.4 -0.2 -0.8
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.3 -0.2 +0.9 -0.2 -0.2
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.2 -0.8 +0.4 +0.5 -0.1
stress LB Anaerobic with Spectinomycin 0.0000625 mM -0.3 -0.4 +0.9 +0.2 -0.3
control PBS_cage_2_mouse_3_Cecum_40 hr -0.3 +0.3 +0.1 -0.7 +0.8
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr +0.6 -0.5 +0.1 +0.4 -0.3
control PBS_cage_2_mouse_1_Cecum_40 hr +0.4 -0.1 -0.1 -0.7 +0.9
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -0.2 +0.8 +0.1 +0.4 -0.4
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.3 +0.8 -0.0 -0.3 +0.4
control PBS_cage_2_mice _Feces_22 hr +0.4 -0.1 +0.0 -0.3 +0.9
stress LB Anaerobic with Hygromycin B 0.00025 mM -0.2 -0.5 +1.6 +0.1 +0.2
stress LB Anaerobic with Cefoxitin 0.000003125 mM +0.5 +1.1 -0.0 -0.2 -0.1
control PBS_cage_2_mice _Feces_40 hr +0.3 +0.2 -0.0 -0.2 +1.1
stress LB Anaerobic with Furaltadone 0.000015625 mM -0.2 -0.0 +0.6 +0.7 +0.3
phage RB68 -0.1 -0.2 +0.5 -0.0 +1.3
stress LB Anaerobic with Cefoxitin 0.000003125 mM +0.2 +1.1 +0.1 -0.2 +0.3
stress Bacitracin 1 mg/ml -0.2 +0.3 +0.7 +0.5 +0.4
stress LB Anaerobic with Hygromycin B 0.00025 mM -0.1 -0.4 +2.1 +0.2 -0.1
control PBS_cage_2_mouse_2_Cecum_40 hr +0.7 +0.2 -0.3 -0.0 +1.3
phage Ste41 +0.9 -0.2 +0.5 +0.1 +1.2
stress Butanol 1.25 vol% +0.0 +0.0 +2.7 +0.2 +0.0
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