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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
mhpE and mhpT are separated by 577 nucleotides
mhpT and yaiL are separated by 101 nucleotides
yaiL and frmB are separated by 223 nucleotides
frmB and frmA are separated by 93 nucleotides
b0352: mhpE - 4-hydroxy-2-ketovalerate aldolase (NCBI), at 373,092 to 374,105
mhpE
b0353: mhpT - putative transport protein (VIMSS), at 374,683 to 375,894
mhpT
b0354: yaiL - nucleoprotein/polynucleotide-associated enzyme (VIMSS), at 375,996 to 376,535
yaiL
b0355: frmB - predicted esterase (NCBI), at 376,759 to 377,592
frmB
b0356: frmA - alcohol dehydrogenase class III/glutathione-dependent formaldehyde dehydrogenase (NCBI), at 377,686 to 378,795
frmA
Group
Condition
b0352
b0353
b0354
b0355
b0356
mhpE
mhpT
yaiL
frmB
frmA
stress
3-methyl-2-butenol 1.25 vol%
-0.1
+0.1
+0.2
-3.1
-1.7
stress
3-methyl-3-butenol 0.625 vol%
+0.2
+0.0
-0.0
-1.0
-3.7
stress
3-methyl-3-butenol 0.625 vol%
+0.2
+0.2
+0.1
-1.0
-3.4
stress
3-methyl-2-butenol 1.25 vol%
-0.1
+0.1
+0.1
-3.2
-0.6
stress
3-methyl-3-butenol 0.31 vol%
+0.2
+0.1
+0.1
-0.6
-2.0
nophagecontrol
R1_N4_T1_phage0
-0.4
-0.7
-0.4
-0.5
-0.2
phage
Ste41
-1.2
-0.6
+0.3
-0.5
-0.1
stress
3-methyl-3-butenol 0.31 vol%
-0.0
-0.0
+0.2
-0.5
-1.3
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.1
-0.4
-0.2
-0.8
-0.3
stress
Cephalothin 0.01 mg/ml
-1.1
-0.1
-0.1
+0.1
-0.4
phage
T7_phage 0.00015625 MOI TC3
+0.6
-0.5
-0.8
-1.0
+0.2
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+0.1
-0.4
-0.4
-0.3
-0.5
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
+0.1
-0.4
-0.3
-0.6
-0.2
stress
3-methyl-2-butenol 0.625 vol%
+0.0
+0.0
+0.1
-0.5
-1.0
stress
LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM
-0.2
-0.4
-0.5
+0.3
-0.2
stress
octanol 0.08 vol%
-0.2
-1.2
-0.1
+0.3
+0.2
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
+0.8
-0.4
-0.3
-0.5
-0.3
stress
Tetracycline 0.0008 mg/ml
+0.3
-1.1
+0.1
+0.0
+0.1
stress
Cisplatin 0.1 mg/ml
+0.1
-0.3
+0.6
-0.4
-0.5
phage
T7_phage 0.00015625 MOI TC4
-1.0
-0.3
+0.2
+0.4
+0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.6
-0.5
-0.8
+0.1
+0.3
carbon source
carbon source D-Glucose 0.25mM
-0.6
-0.6
+0.0
+0.6
+0.4
stress
Nickel (II) chloride 1 mM
+0.2
-0.5
+0.3
-0.1
+0.4
phage
Phage_N4_cage_3_mouse_4_Cecum_40 hr
+0.4
-0.7
+0.6
-0.1
+0.2
predation
6 hr rep1_HD100_10fold_dilution_grewON
-0.2
+0.6
-0.3
+0.4
+0.2
stress
3-methyl-1-butanol 1.25 vol%
+0.3
+0.5
+0.6
+0.1
-0.5
phage
R2_N4_T2_phage10
+0.2
+0.6
+0.1
+0.1
+0.6
control
PBS_cage_1_mice _Feces_16 hr
+0.2
+0.3
+0.7
+0.2
+0.4
micoeukaryotes
Tetrahymena sp., Mixed culture-1
+0.2
+0.7
+0.7
+0.1
+0.3
motility
inner cut, LB soft agar motility assay
+0.2
+0.5
-0.0
+0.5
+1.0
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