Fitness for 5 genes in Escherichia coli BW25113

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500 ntmhpE and mhpT are separated by 577 nucleotidesmhpT and yaiL are separated by 101 nucleotidesyaiL and frmB are separated by 223 nucleotidesfrmB and frmA are separated by 93 nucleotides b0352: mhpE - 4-hydroxy-2-ketovalerate aldolase (NCBI), at 373,092 to 374,105 mhpE b0353: mhpT - putative transport protein (VIMSS), at 374,683 to 375,894 mhpT b0354: yaiL - nucleoprotein/polynucleotide-associated enzyme (VIMSS), at 375,996 to 376,535 yaiL b0355: frmB - predicted esterase (NCBI), at 376,759 to 377,592 frmB b0356: frmA - alcohol dehydrogenase class III/glutathione-dependent formaldehyde dehydrogenase (NCBI), at 377,686 to 378,795 frmA
Group Condition b0352 b0353 b0354 b0355 b0356
mhpE mhpT yaiL frmB frmA
stress 3-methyl-2-butenol 1.25 vol% -0.1 +0.1 +0.2 -3.1 -1.7
stress 3-methyl-3-butenol 0.625 vol% +0.2 +0.0 -0.0 -1.0 -3.7
stress 3-methyl-3-butenol 0.625 vol% +0.2 +0.2 +0.1 -1.0 -3.4
stress 3-methyl-2-butenol 1.25 vol% -0.1 +0.1 +0.1 -3.2 -0.6
stress 3-methyl-3-butenol 0.31 vol% +0.2 +0.1 +0.1 -0.6 -2.0
nophagecontrol R1_N4_T1_phage0 -0.4 -0.7 -0.4 -0.5 -0.2
phage Ste41 -1.2 -0.6 +0.3 -0.5 -0.1
stress 3-methyl-3-butenol 0.31 vol% -0.0 -0.0 +0.2 -0.5 -1.3
stress LB Aerobic with Ramoplanin 0.000125 mM -0.1 -0.4 -0.2 -0.8 -0.3
stress Cephalothin 0.01 mg/ml -1.1 -0.1 -0.1 +0.1 -0.4
phage T7_phage 0.00015625 MOI TC3 +0.6 -0.5 -0.8 -1.0 +0.2
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.1 -0.4 -0.4 -0.3 -0.5
stress LB Anaerobic with Amoxicillin 0.0000078125 mM +0.1 -0.4 -0.3 -0.6 -0.2
stress 3-methyl-2-butenol 0.625 vol% +0.0 +0.0 +0.1 -0.5 -1.0
stress LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM -0.2 -0.4 -0.5 +0.3 -0.2
stress octanol 0.08 vol% -0.2 -1.2 -0.1 +0.3 +0.2
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM +0.8 -0.4 -0.3 -0.5 -0.3
stress Tetracycline 0.0008 mg/ml +0.3 -1.1 +0.1 +0.0 +0.1
stress Cisplatin 0.1 mg/ml +0.1 -0.3 +0.6 -0.4 -0.5
phage T7_phage 0.00015625 MOI TC4 -1.0 -0.3 +0.2 +0.4 +0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.6 -0.5 -0.8 +0.1 +0.3
carbon source carbon source D-Glucose 0.25mM -0.6 -0.6 +0.0 +0.6 +0.4
stress Nickel (II) chloride 1 mM +0.2 -0.5 +0.3 -0.1 +0.4
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr +0.4 -0.7 +0.6 -0.1 +0.2
predation 6 hr rep1_HD100_10fold_dilution_grewON -0.2 +0.6 -0.3 +0.4 +0.2
stress 3-methyl-1-butanol 1.25 vol% +0.3 +0.5 +0.6 +0.1 -0.5
phage R2_N4_T2_phage10 +0.2 +0.6 +0.1 +0.1 +0.6
control PBS_cage_1_mice _Feces_16 hr +0.2 +0.3 +0.7 +0.2 +0.4
micoeukaryotes Tetrahymena sp., Mixed culture-1 +0.2 +0.7 +0.7 +0.1 +0.3
motility inner cut, LB soft agar motility assay +0.2 +0.5 -0.0 +0.5 +1.0
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