Fitness for 5 genes in Escherichia coli BW25113

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500 ntinsA-3 and yagJ are separated by 57 nucleotidesyagJ and yagK are separated by 90 nucleotidesyagK and yagL are separated by 271 nucleotidesyagL and yagM are separated by 26 nucleotides b0275: insA-3 - CP4-6 prophage; IS1 repressor protein InsA (NCBI), at 290,295 to 290,570 insA-3 b0276: yagJ - CP4-6 prophage; predicted protein (RefSeq), at 290,628 to 291,455 yagJ b0277: yagK - CP4-6 prophage; conserved protein (NCBI), at 291,546 to 292,172 yagK b0278: yagL - CP4-6 prophage; DNA-binding protein (NCBI), at 292,444 to 293,142 yagL b0279: yagM - CP4-6 prophage; predicted protein (NCBI), at 293,169 to 294,023 yagM
Group Condition b0275 b0276 b0277 b0278 b0279
insA-3 yagJ yagK yagL yagM
M9 Glucose 2% aerobic N.D. -0.0 -0.0 -0.5 -2.6
M9 Glucose 2%, 20 mM NO3- anaerobic N.D. -0.0 -0.0 -0.5 -2.5
motility outer cut, LB soft agar motility assay N.D. +0.1 -0.2 +0.1 -3.0
M9 Glucose 2% aerobic N.D. -0.0 -0.0 -0.5 -1.9
M9 Glucose 2% anaerobic N.D. +0.0 +0.0 -0.4 -2.1
M9 Acetate 0.5% aerobic N.D. -0.0 -0.1 -0.5 -1.6
M9 Acetate 0.5% aerobic N.D. +0.0 +0.0 -0.6 -1.6
M9 Glucose 2% anaerobic N.D. +0.0 +0.0 -0.4 -1.7
motility inner cut, LB soft agar motility assay N.D. +0.3 +0.0 -0.3 -1.6
stress Benzalkonium Chloride 0.004 mg/ml N.D. +0.2 +0.1 -0.3 -1.4
motility inner cut, LB soft agar motility assay N.D. +0.2 +0.1 +0.2 -1.2
phage Δdbn_10 N.D. +0.2 -0.3 -0.9 +0.9
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. -0.2 -0.1 -0.7 +0.9
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM N.D. +0.1 +0.1 -0.2 +1.3
stress Ciprofloxacin Hydrochloride 0.0003 mM N.D. -0.1 +0.4 -0.2 +1.2
stress Ciprofloxacin Hydrochloride 0.000156 mM N.D. -0.0 -0.1 +0.2 +1.4
phage T7_phage 0.00015625 MOI TC4 N.D. -0.2 +0.2 +0.4 +1.1
stress LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM N.D. +0.2 -0.0 +0.3 +1.2
stress Trimethoprim 0.005 mM N.D. -0.1 +0.1 +0.0 +1.6
stress M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM N.D. +0.1 +0.0 +0.3 +1.3
stress M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM N.D. +0.2 +0.2 +0.2 +1.2
carbon source carbon source D-Mannose 5mM N.D. +0.3 +0.1 -0.3 +1.6
phage Ste41 N.D. +0.0 +0.1 +0.1 +1.7
control PBS_cage_2_mouse_2_Cecum_40 hr N.D. +0.6 +0.1 +0.5 +1.1
phage N4_phage 0.09375 MOI TC1 N.D. +0.2 +0.2 +0.4 +1.4
phage T7_phage 0.00015625 MOI TC3 N.D. +0.3 +0.8 +0.8 +0.5
stress M9 with Methotrexate 1.98 mM 24 hr N.D. -0.1 +0.0 +0.3 +2.3
phage T7_phage 0.0015625 MOI TC2 N.D. -0.1 +0.8 +0.4 +1.5
phage Papa dilution 10-1 cacl2 maltose N.D. -0.0 -0.3 +0.1 +2.8
phage N4_phage 0.009375 MOI TC2 N.D. +0.3 +0.3 +0.4 +2.1
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