Fitness for 5 genes in Escherichia coli BW25113

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500 ntyafW and ykfH are separated by 18 nucleotidesykfH and ykfG are separated by 8 nucleotidesykfG and yafX are separated by 15 nucleotidesyafX and ykfF are separated by 97 nucleotides b0246: yafW - CP4-6 prophage; antitoxin of the YkfI-YafW toxin-antitoxin system (NCBI), at 262,914 to 263,231 yafW b4504: ykfH - hypothetical protein (NCBI), at 263,250 to 263,471 ykfH b0247: ykfG - CP4-6 prophage; predicted DNA repair protein (NCBI), at 263,480 to 263,956 ykfG b0248: yafX - CP4-6 prophage; predicted protein (NCBI), at 263,972 to 264,430 yafX b0249: ykfF - CP4-6 prophage; predicted protein (NCBI), at 264,528 to 264,767 ykfF
Group Condition b0246 b4504 b0247 b0248 b0249
yafW ykfH ykfG yafX ykfF
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr +0.0 -0.9 -3.5 -0.3 -0.4
stress Ciprofloxacin Hydrochloride 0.0024 mM -0.2 -0.5 -5.1 +0.0 +0.8
carbon source carbon source D-Glucose 5mM -0.9 -1.0 -1.5 +0.3 -1.4
stress 3-methyl-1-butanol 1.25 vol% +0.5 -0.5 -2.4 -0.4 -1.2
phage R2_N4_T1_phage10 -0.5 -0.4 -3.0 -0.2 +0.6
phage TP4 -0.3 +0.2 -1.2 -0.3 -1.6
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.3 -0.3 -1.4 -0.5 -1.3
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.2 -0.8 -0.9 -0.0 -1.7
phage T7_phage 0.0015625 MOI TC2 +0.5 +0.3 -4.7 +0.3 +0.5
carbon source Ying_Others16 rep B; time point 2 +0.0 +0.3 -2.8 -0.3 +0.0
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM +0.0 +0.1 -2.3 +0.4 -0.7
carbon source carbon source D-Mannose 0.5mM +0.5 +0.2 -1.1 -0.1 -1.7
stress Chloramphenicol 0.005 mg/ml +0.2 +0.1 +0.3 -0.1 -2.7
phage T7_phage 0.00015625 MOI TC4 +0.5 -0.7 -0.4 -0.6 -0.9
carbon source carbon source D-Mannose 5mM +0.2 -0.9 +0.2 +0.4 -1.8
phage Phage_N4_cage_3_mouse_1_Cecum_40 hr +0.6 +0.5 -1.7 -0.5 +0.1
stress Carbenicillin 0.008 mg/ml +0.5 +0.8 -0.4 -0.1 -1.4
control NoBALOControl +0.2 -0.1 +0.9 +0.4 -1.9
plasmid transformation mariner vector transformation; liquid outgrowth assay -1.1 +1.1 +0.3 -0.2 -0.5
plasmid transformation mariner vector transformation; liquid outgrowth assay -1.0 -0.5 +1.1 -0.1 +0.4
carbon source carbon source D-Mannose 0.5mM +0.2 +0.1 +0.9 +0.4 -1.4
stress octanol 0.08 vol% -0.4 +0.7 +0.5 -1.0 +0.8
phage Ste41 +0.7 +0.2 -0.4 -0.9 +1.3
motility inner cut, LB soft agar motility assay -0.1 +1.3 -1.2 +0.0 +0.8
control PBS_cage_1_mouse_1_Cecum_40 hr +0.5 +1.3 +0.7 -0.3 -1.3
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.5 -0.2 +1.4 +0.4 -1.0
motility inner cut, LB soft agar motility assay +0.4 +0.0 +1.4 +0.5 -0.8
nophagecontrol R1_N4_T1_phage0 +0.5 +1.0 +0.9 +0.7 -0.4
stress Benzalkonium Chloride 0.004 mg/ml -0.3 +0.7 +0.9 +0.3 +1.1
control PBS_cage_1_mice _Feces_16 hr +0.6 +0.8 +0.3 +0.5 +1.0
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