Fitness for 5 genes in Escherichia coli BW25113

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500 ntdkgB and yafC overlap by 4 nucleotidesyafC and yafD are separated by 240 nucleotidesyafD and yafE are separated by 3 nucleotidesyafE and mltD are separated by 47 nucleotides b0207: dkgB - 2,5-diketo-D-gluconate reductase B (NCBI), at 229,167 to 229,970 dkgB b0208: yafC - predicted DNA-binding transcriptional regulator (NCBI), at 229,967 to 230,881 yafC b0209: yafD - hypothetical protein (NCBI), at 231,122 to 231,922 yafD b0210: yafE - predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI), at 231,926 to 232,549 yafE b0211: mltD - predicted membrane-bound lytic murein transglycosylase D (NCBI), at 232,597 to 233,955 mltD
Group Condition b0207 b0208 b0209 b0210 b0211
dkgB yafC yafD yafE mltD
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.0 +0.4 -0.5 -1.0 -2.3
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.2 -0.1 +0.4 -0.5 -2.4
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.1 +0.0 +0.4 -0.6 -2.3
carbon source a-Ketoglutaric (C) +0.0 +0.0 -1.8 +0.2 -0.4
carbon source a-Ketoglutaric (C) -0.3 +0.2 -1.7 +0.1 -0.3
no stress control anaerobic M9 no stress control; Anaerobic 24 hr -0.0 +0.1 -1.8 +0.2 -0.2
stress LB Aerobic with Ramoplanin 0.000125 mM -0.1 +0.1 +0.3 -0.4 -1.6
stress pinene 0.3125 vol% -0.2 +0.1 -1.9 +0.2 +0.1
stress Bacitracin 1 mg/ml +0.4 +0.4 +0.4 -1.4 -1.4
stress limonene 2.5 vol% -0.0 +0.0 -1.8 +0.5 +0.1
stress Benzalkonium Chloride 0.004 mg/ml +0.9 +0.6 -2.5 -0.2 +0.0
stress undecene 5 vol% -0.2 -0.1 -1.3 +0.5 -0.2
gfp_control GFP_control -0.6 -0.6 +0.7 +0.0 -0.5
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.4 -0.3 +1.1 -0.5 -0.9
stress 3-methyl-1-butanol 1.25 vol% +0.1 +0.2 +0.1 -1.6 +0.2
predation 6 hr rep2_HD100_10fold_dilution_grewON +0.4 -0.3 -0.6 -0.8 +0.4
retron_ec_48_control retron_Ec_48_control -0.4 -0.4 +0.9 -0.2 -0.3
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.2 +0.6 -1.8 +0.2 +0.4
carbon source carbon source D-Glucose 0.25mM -0.8 +0.4 -0.5 +1.2 -0.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.6 +0.3 -1.5 +0.3 +0.2
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.0 +1.0 -1.0 +0.0 +0.4
phage T7_phage 0.00015625 MOI TC4 +0.3 -0.2 +0.5 -1.0 +0.9
phage T7_phage 0.0015625 MOI TC1 +0.2 +0.2 +0.7 -1.0 +0.6
stress LB Aerobic with Novobiocin 0.0000625 mM -0.1 -0.1 +1.1 -0.5 +0.6
phage Ste41 +0.2 +0.1 +0.9 -0.8 +0.6
motility outer cut, LB soft agar motility assay +0.2 +0.4 -0.1 +1.1 -0.5
stress Chloramphenicol 0.0025 mg/ml +0.3 -0.4 +0.8 +0.6 +0.4
control PBS_cage_1_mouse_1_Cecum_40 hr +0.5 +0.6 +0.3 +0.8 -0.4
stress LB Aerobic with Novobiocin 0.0000625 mM -0.0 -0.1 +1.0 +0.8 +0.4
stress Cisplatin 0.1 mg/ml +0.1 +0.6 +0.5 +0.7 +0.4
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