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Fitness for 5 genes in
Escherichia coli BW25113
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
dkgB and yafC overlap by 4 nucleotides
yafC and yafD are separated by 240 nucleotides
yafD and yafE are separated by 3 nucleotides
yafE and mltD are separated by 47 nucleotides
b0207: dkgB - 2,5-diketo-D-gluconate reductase B (NCBI), at 229,167 to 229,970
dkgB
b0208: yafC - predicted DNA-binding transcriptional regulator (NCBI), at 229,967 to 230,881
yafC
b0209: yafD - hypothetical protein (NCBI), at 231,122 to 231,922
yafD
b0210: yafE - predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI), at 231,926 to 232,549
yafE
b0211: mltD - predicted membrane-bound lytic murein transglycosylase D (NCBI), at 232,597 to 233,955
mltD
Group
Condition
b0207
b0208
b0209
b0210
b0211
dkgB
yafC
yafD
yafE
mltD
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.0
+0.4
-0.5
-1.0
-2.3
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.2
-0.1
+0.4
-0.5
-2.4
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.1
+0.0
+0.4
-0.6
-2.3
carbon source
a-Ketoglutaric (C)
+0.0
+0.0
-1.8
+0.2
-0.4
carbon source
a-Ketoglutaric (C)
-0.3
+0.2
-1.7
+0.1
-0.3
no stress control anaerobic
M9 no stress control; Anaerobic 24 hr
-0.0
+0.1
-1.8
+0.2
-0.2
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.1
+0.1
+0.3
-0.4
-1.6
stress
pinene 0.3125 vol%
-0.2
+0.1
-1.9
+0.2
+0.1
stress
Bacitracin 1 mg/ml
+0.4
+0.4
+0.4
-1.4
-1.4
stress
limonene 2.5 vol%
-0.0
+0.0
-1.8
+0.5
+0.1
stress
Benzalkonium Chloride 0.004 mg/ml
+0.9
+0.6
-2.5
-0.2
+0.0
stress
undecene 5 vol%
-0.2
-0.1
-1.3
+0.5
-0.2
gfp_control
GFP_control
-0.6
-0.6
+0.7
+0.0
-0.5
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-0.4
-0.3
+1.1
-0.5
-0.9
stress
3-methyl-1-butanol 1.25 vol%
+0.1
+0.2
+0.1
-1.6
+0.2
predation
6 hr rep2_HD100_10fold_dilution_grewON
+0.4
-0.3
-0.6
-0.8
+0.4
retron_ec_48_control
retron_Ec_48_control
-0.4
-0.4
+0.9
-0.2
-0.3
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.2
+0.6
-1.8
+0.2
+0.4
carbon source
carbon source D-Glucose 0.25mM
-0.8
+0.4
-0.5
+1.2
-0.6
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.6
+0.3
-1.5
+0.3
+0.2
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.0
+1.0
-1.0
+0.0
+0.4
phage
T7_phage 0.00015625 MOI TC4
+0.3
-0.2
+0.5
-1.0
+0.9
phage
T7_phage 0.0015625 MOI TC1
+0.2
+0.2
+0.7
-1.0
+0.6
stress
LB Aerobic with Novobiocin 0.0000625 mM
-0.1
-0.1
+1.1
-0.5
+0.6
phage
Ste41
+0.2
+0.1
+0.9
-0.8
+0.6
motility
outer cut, LB soft agar motility assay
+0.2
+0.4
-0.1
+1.1
-0.5
stress
Chloramphenicol 0.0025 mg/ml
+0.3
-0.4
+0.8
+0.6
+0.4
control
PBS_cage_1_mouse_1_Cecum_40 hr
+0.5
+0.6
+0.3
+0.8
-0.4
stress
LB Aerobic with Novobiocin 0.0000625 mM
-0.0
-0.1
+1.0
+0.8
+0.4
stress
Cisplatin 0.1 mg/ml
+0.1
+0.6
+0.5
+0.7
+0.4
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