Fitness for 5 genes in Escherichia coli BW25113

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500 ntproS and yaeB are separated by 111 nucleotidesyaeB and rcsF overlap by 4 nucleotidesrcsF and metQ are separated by 117 nucleotidesmetQ and metI are separated by 39 nucleotides b0194: proS - prolyl-tRNA synthetase (NCBI), at 217,057 to 218,775 proS b0195: yaeB - hypothetical protein (NCBI), at 218,887 to 219,594 yaeB b0196: rcsF - predicted outer membrane protein, signal (NCBI), at 219,591 to 219,995 rcsF b0197: metQ - DL-methionine transporter subunit (NCBI), at 220,113 to 220,928 metQ b0198: metI - DL-methionine transporter subunit (NCBI), at 220,968 to 221,621 metI
Group Condition b0194 b0195 b0196 b0197 b0198
proS yaeB rcsF metQ metI
phage Laval1374 N.D. -0.3 -6.7 -0.1 +0.2
phage Laval1374 N.D. -0.3 -5.8 -0.2 +0.2
stress Chloride 750 mM N.D. -0.1 -3.7 +0.3 -0.1
carbon source acetate (C) N.D. +0.1 -0.0 -1.4 -1.0
M9 Acetate 0.5% aerobic N.D. -0.0 +0.2 -1.0 -1.3
stress LB Anaerobic with Cephalothin 0.0000078125 mM N.D. -0.2 -0.7 -0.5 -0.5
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr N.D. -0.9 -0.4 -0.3 -0.2
M9 Acetate 0.5% aerobic N.D. +0.3 +0.2 -1.1 -1.2
phage T7_phage 0.00015625 MOI TC3 N.D. +0.0 -0.8 -0.8 -0.2
predation 48 hr rep2_HD100_10fold_dilution N.D. -0.1 +0.1 -0.8 -0.9
carbon source Glycolic Acid (C) N.D. +0.5 +0.3 -1.1 -1.3
carbon source acetate (C) N.D. +0.3 +0.1 -0.9 -0.8
carbon source Acetate (C) N.D. +0.2 -0.1 -0.8 -0.5
stress Bacitracin 1 mg/ml N.D. -0.3 -1.3 -0.1 +0.5
phage Ste41 N.D. -0.7 -0.9 +0.3 +0.2
carbon source succinate (C) N.D. +0.2 +0.3 -1.0 -0.6
stress Trimethoprim 0.00125 mM N.D. -0.5 -0.8 +0.4 -0.1
carbon source Glycolic Acid (C) N.D. +0.2 +0.7 -0.6 -1.1
stress Cephalothin 0.01 mg/ml N.D. +0.2 -0.8 +0.3 -0.4
stress LB Anaerobic with Amoxicillin 0.0000078125 mM N.D. -0.2 -0.9 +0.6 -0.1
phage Ste41 N.D. -0.5 -0.9 +0.9 +0.3
carbon source L-Malic (C) N.D. +0.5 +0.5 -0.7 -0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. +0.4 +0.6 -0.7 +0.5
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. +0.3 +0.3 -0.5 +0.6
phage T7_phage 0.0015625 MOI TC2 N.D. +0.3 +0.4 +0.7 -0.4
carbon source Ying_Nucleobase14 rep C; time point 3 N.D. +0.2 -0.2 +0.9 +0.5
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM N.D. +0.7 +0.6 -0.0 +0.4
carbon source Ying_Nucleobase14 rep A; time point 3 N.D. +0.3 +0.3 +0.9 +0.3
stress Benzalkonium Chloride 0.004 mg/ml N.D. +0.9 +0.5 +0.2 +0.4
nitrogen source Putrescine (N) N.D. +0.6 +0.1 +0.5 +0.8
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