Fitness for 5 genes in Escherichia coli BW25113

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500 ntyaeR and tilS are separated by 64 nucleotidestilS and yaeO are separated by 48 nucleotidesyaeO and yaeP overlap by 8 nucleotidesyaeP and yaeQ are separated by 165 nucleotides b0187: yaeR - orf, hypothetical protein (VIMSS), at 211,877 to 212,266 yaeR b0188: tilS - tRNA(Ile)-lysidine synthetase (NCBI), at 212,331 to 213,629 tilS b0189: yaeO - orf, hypothetical protein (VIMSS), at 213,678 to 213,932 yaeO b4406: yaeP - hypothetical protein (NCBI), at 213,925 to 214,125 yaeP b0190: yaeQ - hypothetical protein (NCBI), at 214,291 to 214,836 yaeQ
Group Condition b0187 b0188 b0189 b4406 b0190
yaeR tilS yaeO yaeP yaeQ
carbon source carbon source D-Glucose 5mM +0.4 N.D. -0.5 -4.2 +0.4
carbon source carbon source D-Glucose 0.25mM -0.5 N.D. -0.7 -2.5 -0.2
micoeukaryotes Tetrahymena sp., Mixed culture-1 -2.7 N.D. -0.2 -0.8 +0.3
stress Benzalkonium Chloride 0.004 mg/ml -0.2 N.D. +0.4 -3.1 -0.4
stress octanol 0.08 vol% -0.5 N.D. +0.5 -1.9 -0.7
stress LB Aerobic with Ramoplanin 0.000125 mM -0.3 N.D. -0.4 -2.1 +0.9
stress Cephalothin 0.01 mg/ml -0.5 N.D. -0.7 -0.8 +1.3
phage Ste41 +0.6 N.D. +0.4 -2.1 +0.6
carbon source D-Maltose (C) -0.4 N.D. -1.2 +1.1 +0.5
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -1.9 N.D. -0.2 +1.1 +1.2
carbon source D-Ribose (C) +0.1 N.D. -1.6 +0.9 +0.9
carbon source L-Malic (C) -0.1 N.D. -2.0 +1.7 +0.9
carbon source D-Ribose (C) -0.1 N.D. -1.3 +1.1 +1.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.8 N.D. -1.1 -0.4 +1.6
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.8 N.D. +0.7 -0.7 +1.7
carbon source D-Xylose (C) -0.1 N.D. -1.1 +1.5 +0.7
nitrogen source Gly-Glu (N) -0.6 N.D. -0.8 +1.7 +1.0
carbon source succinate (C) -0.2 N.D. -1.0 +1.8 +0.7
carbon source succinate (C) +0.2 N.D. -1.1 +1.4 +0.9
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) +0.2 N.D. -1.1 +1.4 +0.9
nitrogen source Gly-DL-Asp (N) -0.0 N.D. -1.0 +1.8 +0.6
carbon source L-Malic (C) -0.2 N.D. -1.3 +1.9 +1.0
nitrogen source Gly-DL-Asp (N) +0.1 N.D. -1.0 +1.6 +0.8
carbon source LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) +0.3 N.D. -0.9 +1.5 +0.7
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.8 N.D. +0.4 +1.0 +1.4
carbon source LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) +0.4 N.D. -0.7 +1.8 +0.8
nitrogen source Gly-Glu (N) +0.1 N.D. -0.7 +1.7 +1.2
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) +0.2 N.D. -0.7 +1.8 +1.1
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.0 N.D. -0.2 +0.6 +1.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.8 N.D. -0.9 +2.3 +1.8
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