Fitness for 5 genes in Escherichia coli BW25113

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500 nthrpB and mrcB are separated by 195 nucleotidesmrcB and fhuA are separated by 219 nucleotidesfhuA and fhuC are separated by 50 nucleotidesfhuC and fhuD overlap by 1 nucleotides b0148: hrpB - helicase, ATP-dependent (VIMSS), at 162,105 to 164,534 hrpB b0149: mrcB - penicillin-binding protein 1b (NCBI), at 164,730 to 167,264 mrcB b0150: fhuA - ferrichrome outer membrane transporter (NCBI), at 167,484 to 169,727 fhuA b0151: fhuC - iron-hydroxamate transporter subunit (NCBI), at 169,778 to 170,575 fhuC b0152: fhuD - iron-hydroxamate transporter subunit (NCBI), at 170,575 to 171,465 fhuD
Group Condition b0148 b0149 b0150 b0151 b0152
hrpB mrcB fhuA fhuC fhuD
supernatant LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x +0.0 -5.7 -0.4 -0.5 -0.7
supernatant LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x +0.0 -5.5 -0.5 -0.5 -0.7
ecofab LB 0.5x in EcoFAB_3.5mL +0.2 -7.0 -0.2 +0.1 -0.0
mixed community LB 0.1x with Mixed culture; Trichoderma harzianum M10 +0.1 -7.1 +0.0 +0.1 +0.0
control Mixed culture control experiment +0.0 -7.0 +0.1 +0.1 -0.0
micoeukaryotes Tetrahymena sp., Mixed culture-1 +0.2 -6.4 -0.1 +0.1 -0.2
mixed community LB 0.1x with Mixed culture; Trichoderma virens G41 +0.0 -6.3 +0.0 -0.1 +0.1
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x -0.0 -3.1 -0.9 -1.3 -1.0
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x +0.0 -3.2 -0.8 -1.2 -1.0
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x +0.1 -5.0 -0.3 -0.5 -0.4
control Mixed culture control experiment +0.1 -6.3 -0.0 +0.1 -0.0
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x +0.0 -3.7 -0.5 -1.1 -0.8
control Mixed culture control experiment +0.1 -6.0 -0.0 +0.1 +0.1
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x +0.1 -4.9 -0.1 -0.3 -0.2
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x +0.2 -4.5 -0.3 -0.5 -0.3
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x +0.1 -4.9 -0.1 -0.3 -0.1
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x +0.0 -3.1 -0.5 -1.0 -0.7
micoeukaryotes Acanthamoeba sp., muxed culture-2 -0.1 -5.3 -0.1 +0.2 +0.0
stress D-Cycloserine 0.1 mg/ml -0.0 -5.2 +0.2 +0.1 -0.2
stress LB Anaerobic with Azlocillin 0.00003125 mM +0.4 -4.9 -0.0 +0.1 -0.1
nitrogen source D-Serine (N) -0.1 -4.8 +0.1 +0.2 +0.3
stress LB Aerobic with Ramoplanin 0.000125 mM +0.2 -4.7 +0.5 +0.4 +0.1
stress LB Anaerobic with Amoxicillin 0.0000078125 mM +1.6 -3.9 +0.0 +0.2 -0.4
stress LB Anaerobic with Cephalothin 0.0000078125 mM +2.6 -4.1 +0.2 -0.4 -0.5
phage Laval1374 +0.4 -4.1 +1.5 +0.2 -0.1
stress LB Anaerobic with Amoxicillin 0.0000078125 mM +2.0 -3.3 -0.2 +0.0 -0.3
stress LB Anaerobic with Cephalothin 0.0000078125 mM +2.7 -3.9 -0.0 -0.2 -0.2
stress LB Anaerobic with Cefoxitin 0.000003125 mM +3.9 -3.2 -0.2 -0.3 -0.1
stress LB Anaerobic with Cefoxitin 0.000003125 mM +3.9 -3.6 +0.1 -0.1 -0.1
phage NoPhageControl_ML9a +0.1 -0.2 +7.0 +0.2 -0.1
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