Fitness for 5 genes in Escherichia coli BW25113

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500 ntyadC and yadK are separated by 49 nucleotidesyadK and yadL are separated by 26 nucleotidesyadL and yadM are separated by 11 nucleotidesyadM and htrE are separated by 16 nucleotides b0135: yadC - predicted fimbrial-like adhesin protein (NCBI), at 149,715 to 150,953 yadC b0136: yadK - predicted fimbrial-like adhesin protein (NCBI), at 151,003 to 151,599 yadK b0137: yadL - predicted fimbrial-like adhesin protein (NCBI), at 151,626 to 152,231 yadL b0138: yadM - putative fimbrial-like protein (VIMSS), at 152,243 to 152,812 yadM b0139: htrE - predicted outer membrane usher protein (NCBI), at 152,829 to 155,426 htrE
Group Condition b0135 b0136 b0137 b0138 b0139
yadC yadK yadL yadM htrE
M9 Acetate 0.5% aerobic -0.4 -1.4 -0.6 -0.9 -0.2
M9 Glucose 2% aerobic -0.3 -1.6 -0.6 -0.7 -0.1
M9 Glucose 2%, 20 mM NO3- anaerobic -0.4 -1.3 -0.6 -0.7 -0.2
M9 Glucose 2% anaerobic -0.3 -1.3 -0.7 -0.7 -0.2
M9 Acetate 0.5% aerobic -0.3 -1.3 -0.4 -0.6 -0.2
predation 3 hr rep1_Tiberius_100fold_dilution -0.7 -0.6 -0.5 -0.7 -0.2
retron_ec_48_control retron_Ec_48_control -0.5 -0.6 -0.4 -0.9 -0.3
M9 Glucose 2% aerobic -0.3 -1.1 -0.5 -0.6 -0.1
motility outer cut, LB soft agar motility assay -0.3 -1.3 -0.6 -0.3 -0.0
motility outer cut, LB soft agar motility assay -0.3 -1.0 -0.7 -0.3 -0.1
motility inner cut, LB soft agar motility assay -0.4 -1.1 -0.4 -0.5 +0.0
mixed community Mixed culture; Bdellovibrio bacteriovorus HD100 1:1 starting OD to Ecoli; 0.1X M9; outgrowth in LBwithKan -0.5 -0.6 -0.5 -0.6 -0.3
carbon source carbon source D-Mannose 0.5mM -0.2 -1.0 -0.1 -0.8 -0.1
retron_ec_48_control retron_Ec_48_control -0.4 -0.5 -0.6 -0.5 -0.2
carbon source carbon source D-Glucose 0.25mM -0.9 +0.1 -0.4 -0.6 -0.4
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr -0.0 -0.9 -0.3 -0.8 -0.2
predation 3 hr rep1_Tiberius_10fold_dilution -0.5 -0.4 -0.5 -0.6 -0.2
M9 Glucose 2% anaerobic -0.3 -0.8 -0.4 -0.5 -0.1
mixed community Mixed culture; Bdellovibrio bacteriovorus HD100 1:10 starting OD to Ecoli; 0.1X M9; outgrowth in LBwithKan -0.4 -0.5 -0.5 -0.4 -0.2
motility inner cut, LB soft agar motility assay -0.3 -0.7 -0.5 -0.4 -0.1
carbon source carbon source D-Mannose 0.5mM -0.2 -1.1 -0.0 -0.7 +0.0
predation 3 hr rep1_HD100_no_dilution -0.5 -0.4 -0.3 -0.6 -0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.6 +0.2 -0.2 -0.8 -0.2
control PBS_cage_1_mice _Feces_40 hr +0.3 +0.8 -0.5 +0.3 -0.0
stress LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM +0.4 +0.7 +0.5 +0.5 +0.3
phage T7_phage 0.00015625 MOI TC4 +0.5 +0.9 +0.4 +0.6 +0.2
stress LB Aerobic with Irgasan 0.0000003125 mM +0.5 +0.8 +0.6 +0.6 +0.2
phage Papa dilution 10-1 cacl2 maltose +0.4 +2.0 +0.4 +0.6 -0.1
phage T7_phage 0.0015625 MOI TC2 +0.6 +0.6 +0.8 +0.9 +0.6
phage T7_phage 0.00015625 MOI TC3 +0.3 +0.4 +1.1 +1.2 +0.9
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