Fitness for 5 genes in Escherichia coli BW25113

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500 ntyacL and speD are separated by 37 nucleotidesspeD and speE are separated by 15 nucleotidesspeE and yacC are separated by 105 nucleotidesyacC and cueO are separated by 165 nucleotides b0119: yacL - orf, hypothetical protein (VIMSS), at 134,388 to 134,750 yacL b0120: speD - S-adenosylmethionine decarboxylase proenzyme (NCBI), at 134,788 to 135,582 speD b0121: speE - spermidine synthase (NCBI), at 135,598 to 136,464 speE b0122: yacC - orf, hypothetical protein (VIMSS), at 136,570 to 136,917 yacC b0123: cueO - multicopper oxidase (laccase) (NCBI), at 137,083 to 138,633 cueO
Group Condition b0119 b0120 b0121 b0122 b0123
yacL speD speE yacC cueO
carbon source Ying_AminoAcid20 rep C; time point 3 -0.0 -1.8 -2.1 -0.0 +0.0
carbon source Ying_AminoAcid20 rep A; time point 3 -0.1 -1.7 -2.2 -0.1 +0.2
msc 22hr_mediaonly_rep2_lowinoculum -0.3 -1.3 -1.3 -0.1 +0.0
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.1 -0.1 -2.3 -0.5 +0.1
carbon source Ying_AminoAcid20 rep B; time point 3 -0.1 -1.7 -1.5 +0.4 -0.0
msc 22hr_mediaonly_rep1_lowinoculum -0.2 -1.3 -1.2 +0.2 +0.1
stress Benzalkonium Chloride 0.004 mg/ml +0.1 -0.3 +0.4 -2.6 +0.2
msc 3hr_mediaonly_rep2_lowinoculum -0.1 -0.8 -1.6 +0.5 +0.0
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -0.1 -0.0 +0.7 -2.4 +0.0
phage T7_phage 0.0015625 MOI TC2 +0.7 +0.1 -0.9 -1.0 -0.3
phage phage +0.7 +0.2 -0.5 -1.2 -0.3
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.0 -0.2 +1.0 -2.2 +0.3
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -0.1 +0.1 +0.9 -1.9 +0.1
stress LB Anaerobic with Ampicillin 0.00000195313 mM -0.4 -0.4 +0.7 +1.3 +0.1
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.4 -0.0 +0.1 +2.1 -0.5
stress Cisplatin 0.1 mg/ml +0.6 -0.5 +1.1 +0.4 -0.2
stress Carbenicillin 0.008 mg/ml -0.3 +0.2 -0.4 +2.6 -0.0
stress Cephalothin 0.01 mg/ml -0.5 +0.1 -0.1 +2.8 -0.0
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) -0.2 +1.5 +1.5 -0.5 +0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.0 -0.0 +1.5 +1.4 -0.1
carbon source LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) -0.3 +1.6 +1.7 -0.4 +0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.3 +0.2 +1.1 +1.4 -0.1
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.3 +0.2 +0.3 +3.4 +0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.4 +0.8 +1.4 +1.3 -0.2
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.6 +0.3 +0.4 +3.4 +0.3
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) -0.0 +1.9 +2.1 -0.4 +0.3
carbon source LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) -0.0 +1.8 +2.3 -0.3 +0.2
carbon source LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) -0.0 +2.1 +2.0 -0.1 +0.4
stress Bacitracin 1 mg/ml +0.3 +0.1 -0.2 +4.7 -0.3
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) +0.0 +2.0 +2.4 +0.1 +0.2
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