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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yacL and speD are separated by 37 nucleotides
speD and speE are separated by 15 nucleotides
speE and yacC are separated by 105 nucleotides
yacC and cueO are separated by 165 nucleotides
b0119: yacL - orf, hypothetical protein (VIMSS), at 134,388 to 134,750
yacL
b0120: speD - S-adenosylmethionine decarboxylase proenzyme (NCBI), at 134,788 to 135,582
speD
b0121: speE - spermidine synthase (NCBI), at 135,598 to 136,464
speE
b0122: yacC - orf, hypothetical protein (VIMSS), at 136,570 to 136,917
yacC
b0123: cueO - multicopper oxidase (laccase) (NCBI), at 137,083 to 138,633
cueO
Group
Condition
b0119
b0120
b0121
b0122
b0123
yacL
speD
speE
yacC
cueO
carbon source
Ying_AminoAcid20 rep C; time point 3
-0.0
-1.8
-2.1
-0.0
+0.0
carbon source
Ying_AminoAcid20 rep A; time point 3
-0.1
-1.7
-2.2
-0.1
+0.2
msc
22hr_mediaonly_rep2_lowinoculum
-0.3
-1.3
-1.3
-0.1
+0.0
micoeukaryotes
Tetrahymena sp., Mixed culture-1
-0.1
-0.1
-2.3
-0.5
+0.1
carbon source
Ying_AminoAcid20 rep B; time point 3
-0.1
-1.7
-1.5
+0.4
-0.0
msc
22hr_mediaonly_rep1_lowinoculum
-0.2
-1.3
-1.2
+0.2
+0.1
stress
Benzalkonium Chloride 0.004 mg/ml
+0.1
-0.3
+0.4
-2.6
+0.2
msc
3hr_mediaonly_rep2_lowinoculum
-0.1
-0.8
-1.6
+0.5
+0.0
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
-0.1
-0.0
+0.7
-2.4
+0.0
phage
T7_phage 0.0015625 MOI TC2
+0.7
+0.1
-0.9
-1.0
-0.3
phage
phage
+0.7
+0.2
-0.5
-1.2
-0.3
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.0
-0.2
+1.0
-2.2
+0.3
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
-0.1
+0.1
+0.9
-1.9
+0.1
stress
LB Anaerobic with Ampicillin 0.00000195313 mM
-0.4
-0.4
+0.7
+1.3
+0.1
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.4
-0.0
+0.1
+2.1
-0.5
stress
Cisplatin 0.1 mg/ml
+0.6
-0.5
+1.1
+0.4
-0.2
stress
Carbenicillin 0.008 mg/ml
-0.3
+0.2
-0.4
+2.6
-0.0
stress
Cephalothin 0.01 mg/ml
-0.5
+0.1
-0.1
+2.8
-0.0
carbon source
LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)
-0.2
+1.5
+1.5
-0.5
+0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.0
-0.0
+1.5
+1.4
-0.1
carbon source
LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C)
-0.3
+1.6
+1.7
-0.4
+0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.3
+0.2
+1.1
+1.4
-0.1
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.3
+0.2
+0.3
+3.4
+0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.4
+0.8
+1.4
+1.3
-0.2
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.6
+0.3
+0.4
+3.4
+0.3
carbon source
LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)
-0.0
+1.9
+2.1
-0.4
+0.3
carbon source
LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C)
-0.0
+1.8
+2.3
-0.3
+0.2
carbon source
LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C)
-0.0
+2.1
+2.0
-0.1
+0.4
stress
Bacitracin 1 mg/ml
+0.3
+0.1
-0.2
+4.7
-0.3
carbon source
LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)
+0.0
+2.0
+2.4
+0.1
+0.2
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