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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
ppdD and nadC are separated by 202 nucleotides
nadC and ampD are separated by 87 nucleotides
ampD and ampE overlap by 4 nucleotides
ampE and aroP are separated by 42 nucleotides
b0108: ppdD - predicted major pilin subunit (NCBI), at 117,109 to 117,549
ppdD
b0109: nadC - nicotinate-nucleotide pyrophosphorylase (NCBI), at 117,752 to 118,645
nadC
b0110: ampD - N-acetyl-anhydromuranmyl-L-alanine amidase (NCBI), at 118,733 to 119,284
ampD
b0111: ampE - predicted inner membrane protein (NCBI), at 119,281 to 120,135
ampE
b0112: aroP - aromatic amino acid transporter (NCBI), at 120,178 to 121,551
aroP
Group
Condition
b0108
b0109
b0110
b0111
b0112
ppdD
nadC
ampD
ampE
aroP
carbon source
D-Glucose-6-Phosphate (C)
-0.3
-2.9
-0.3
-0.2
-0.1
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.4
-0.0
-3.1
-0.2
-0.0
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.3
+0.2
-3.6
+0.2
-0.1
carbon source
casaminos (C)
-0.1
-2.9
-0.2
-0.0
+0.1
nitrogen source
Adenosine (N)
+0.0
-2.9
-0.3
-0.0
-0.0
carbon source
D-Glucose-6-Phosphate (C)
-0.0
-2.6
-0.1
-0.2
-0.2
nitrogen source
Adenosine (N)
-0.1
-2.5
-0.4
-0.2
+0.1
carbon source
Ying_Nucleobase14 rep C; time point 3
+0.0
-2.2
-0.1
-0.2
-0.5
protonated/deuterated
deuterated glucose in H2O
-0.6
-1.9
+0.0
-0.3
-0.2
nitrogen source
Cytidine (N)
-0.2
-2.7
-0.2
+0.1
+0.1
carbon source
Ying_Nucleobase14 rep A; time point 3
-0.3
-2.0
-0.2
+0.0
-0.4
carbon source
L-Malic (C)
-0.2
-2.1
-0.1
-0.2
-0.2
carbon source
D-Galacturonic Acid (C) MOPS
-0.0
-2.4
-0.3
+0.0
-0.1
carbon source
D-Galacturonic Acid (C) M9
-0.1
-2.1
-0.2
+0.0
-0.3
carbon source
D-Glucose (C) MOPS
-0.7
-2.0
-0.0
+0.0
+0.0
carbon source
Ying_Nucleobase14 rep B; time point 3
-0.1
-2.0
-0.1
-0.1
-0.3
nitrogen source
Cytidine (N)
+0.2
-2.7
-0.3
+0.1
+0.0
carbon source
succinate (C)
-0.3
-1.4
-0.4
-0.1
-0.4
M9 Glucose 2% aerobic
-0.0
-2.3
-0.1
-0.2
-0.0
carbon source
D-Glucose (C) MOPS
-0.2
-2.1
-0.2
+0.0
-0.0
carbon source
D-Galacturonic Acid (C) MOPS
+0.1
-2.0
-0.2
-0.1
-0.1
carbon source
L-Malic (C)
+0.4
-2.1
-0.3
-0.2
-0.2
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
-0.3
+0.3
-1.8
+0.0
-0.2
carbon source
casaminos (C)
+0.4
-2.6
+0.1
-0.0
+0.2
protonated/deuterated
protonated glucose in D2O
+0.4
-1.9
-0.1
+0.1
-0.3
stress
Cephalothin 0.01 mg/ml
+0.1
+0.3
-1.7
-0.2
-0.4
stress
Cisplatin 0.1 mg/ml
+0.8
+0.2
-1.0
+0.3
-0.6
stress
LB Aerobic with Doxycycline hyclate 0.00000625 mM
-1.0
+0.4
+0.9
-0.1
-0.1
stress
octanol 0.08 vol%
-0.1
+0.3
+2.4
-0.1
+0.3
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.5
+0.9
+1.8
+0.1
-0.2
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