Fitness for 5 genes in Escherichia coli BW25113

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500 ntftsW and murG overlap by 4 nucleotidesmurG and murC are separated by 53 nucleotidesmurC and ddlB overlap by 8 nucleotidesddlB and ftsQ are separated by 1 nucleotides b0089: ftsW - integral membrane protein involved in stabilizing FstZ ring during cell division (NCBI), at 98,403 to 99,647 ftsW b0090: murG - N-acetylglucosaminyl transferase (NCBI), at 99,644 to 100,711 murG b0091: murC - UDP-N-acetylmuramate--L-alanine ligase (NCBI), at 100,765 to 102,240 murC b0092: ddlB - D-alanylalanine synthetase (NCBI), at 102,233 to 103,153 ddlB b0093: ftsQ - membrane anchored protein involved in growth of wall at septum (NCBI), at 103,155 to 103,985 ftsQ
Group Condition b0089 b0090 b0091 b0092 b0093
ftsW murG murC ddlB ftsQ
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. N.D. N.D. -5.2 N.D.
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. N.D. N.D. -4.4 N.D.
stress D-Cycloserine 0.1 mg/ml N.D. N.D. N.D. -4.1 N.D.
stress Bacitracin 1 mg/ml N.D. N.D. N.D. -3.2 N.D.
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM N.D. N.D. N.D. -2.9 N.D.
stress octanol 0.08 vol% N.D. N.D. N.D. -2.6 N.D.
stress Cephalothin 0.01 mg/ml N.D. N.D. N.D. -2.4 N.D.
stress LB Aerobic with Spiramycin 0.000125 mM N.D. N.D. N.D. -2.1 N.D.
carbon source Glycolic Acid (C) N.D. N.D. N.D. -2.0 N.D.
stress benzenesulfonyl fluoride N.D. N.D. N.D. -1.8 N.D.
stress LB Anaerobic with Cephalothin 0.0000078125 mM N.D. N.D. N.D. -1.8 N.D.
stress 2,4-Dinitrophenol 2 mM N.D. N.D. N.D. -1.7 N.D.
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM N.D. N.D. N.D. -1.7 N.D.
stress LB Aerobic with Spiramycin 0.000125 mM N.D. N.D. N.D. -1.7 N.D.
carbon source carbon source D-Glucose 0.25mM N.D. N.D. N.D. -1.7 N.D.
stress 3-methyl-2-butenol 1.25 vol% N.D. N.D. N.D. +1.6 N.D.
carbon source carbon source D-Mannose 20mM N.D. N.D. N.D. +1.6 N.D.
carbon source carbon source D-Mannose 5mM N.D. N.D. N.D. +1.7 N.D.
stress Cisplatin 0.05 mg/ml N.D. N.D. N.D. +1.7 N.D.
phage T7_phage 0.0015625 MOI TC2 N.D. N.D. N.D. +1.7 N.D.
carbon source carbon source D-Mannose 1mM N.D. N.D. N.D. +1.7 N.D.
carbon source carbon source D-Glucose 20mM N.D. N.D. N.D. +1.8 N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. N.D. N.D. +1.8 N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. N.D. N.D. +1.9 N.D.
carbon source carbon source D-Mannose 5mM N.D. N.D. N.D. +2.0 N.D.
stress Cisplatin 0.1 mg/ml N.D. N.D. N.D. +2.1 N.D.
stress Cisplatin 0.1 mg/ml N.D. N.D. N.D. +2.1 N.D.
stress Cisplatin 0.1 mg/ml N.D. N.D. N.D. +2.3 N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. N.D. N.D. +2.4 N.D.
stress Cisplatin 0.1 mg/ml N.D. N.D. N.D. +2.6 N.D.
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