Fitness for 5 genes in Escherichia coli BW25113

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500 ntleuA and leuL are separated by 92 nucleotidesleuL and leuO are separated by 659 nucleotidesleuO and ilvI are separated by 317 nucleotidesilvI and ilvH are separated by 2 nucleotides b0074: leuA - 2-isopropylmalate synthase (NCBI), at 81,958 to 83,529 leuA b0075: leuL - leu operon leader peptide (NCBI), at 83,622 to 83,708 leuL b0076: leuO - probable transcriptional activator for leuABCD operon (VIMSS), at 84,368 to 85,312 leuO b0077: ilvI - acetolactate synthase III large subunit (NCBI), at 85,630 to 87,354 ilvI b0078: ilvH - acetolactate synthase small subunit (NCBI), at 87,357 to 87,848 ilvH
Group Condition b0074 b0075 b0076 b0077 b0078
leuA leuL leuO ilvI ilvH
carbon source Ying_OrganicAcid7 rep A; time point 3 -8.8 -2.3 -1.0 -7.4 -7.3
carbon source Ying_OrganicAcid7 rep C; time point 3 -8.7 -2.1 -0.9 -6.6 -8.0
carbon source Ying_OrganicAcid7 rep B; time point 3 -8.1 -1.9 -0.9 -7.7 -6.1
carbon source Ying_sugar7 rep A; time point 3 -9.0 -1.9 -0.1 -6.3 -4.9
carbon source Ying_sugar7 rep C; time point 3 -8.9 -2.0 -0.1 -6.0 -4.4
carbon source Ying_sugar7 rep B; time point 3 -9.1 -2.2 -0.0 -5.3 -4.7
carbon source Ying_OrganicAcid7 rep B; time point 2 -6.7 -1.5 -0.8 -6.0 -5.2
carbon source Ying_OrganicAcid7 rep C; time point 2 -6.4 -1.9 -0.8 -5.5 -5.2
carbon source Ying_OrganicAcid7 rep A; time point 2 -6.6 -1.8 -0.8 -4.5 -5.7
nitrogen source D-Serine (N) -5.2 -2.2 -0.7 -4.7 -6.4
carbon source carbon source D-Glucose 5mM -7.5 -3.0 -0.3 -2.9 -3.8
carbon source D-Glucose-6-Phosphate (C) -5.9 -1.7 -0.4 -4.8 -4.5
nitrogen source D-Serine (N) -4.9 -2.4 -0.6 -4.4 -5.0
carbon source Ying_sugar7 rep A; time point 2 -8.9 -1.9 -0.0 -3.4 -2.9
carbon source D-Galacturonic Acid (C) -5.6 -0.9 -1.1 -4.9 -4.5
nutrient M9 Glucose 0.2%, Thauer's vit mix without biotin -6.6 -1.8 -0.3 -3.7 -4.2
nutrient M9 Glucose 0.2%, Thauer's vit mix without biotin with Avidin -6.8 -1.8 -0.4 -3.5 -4.2
carbon source D-Serine (C) -3.4 -3.6 -1.5 -4.2 -3.9
carbon source Ying_sugar7 rep B; time point 2 -9.0 -1.9 -0.1 -2.8 -2.6
carbon source Ying_sugar7 rep C; time point 2 -8.0 -1.8 -0.1 -3.4 -3.1
carbon source D-Glucose-6-Phosphate (C) -5.6 -1.9 -0.4 -3.8 -4.5
nutrient M9 Glucose 0.2%, Thauer's vit mix without biotin -6.5 -1.6 -0.3 -4.0 -3.8
nutrient M9 Glucose 0.2%, Thauer's vit mix -6.1 -1.6 -0.3 -4.0 -4.1
carbon source carbon source D-Glucose 20mM -6.1 -1.4 -0.3 -4.3 -4.0
nitrogen source D-Alanine (N) -5.4 -1.7 -0.4 -4.1 -4.3
nutrient M9 Glucose 0.2%, Thauer's vit mix without biotin with Avidin -6.0 -1.7 -0.3 -4.0 -3.8
carbon source D-Galacturonic Acid (C) MOPS -4.8 -1.3 -0.9 -3.9 -4.8
nutrient M9 Glucose 0.2%, Thauer's vit mix -6.4 -1.5 -0.3 -3.6 -3.9
carbon source D-Galacturonic Acid (C) M9 -4.9 -1.6 -0.8 -3.9 -4.3
carbon source D-Gluconic Acid (C) -6.1 -1.8 -0.7 -3.3 -3.5
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