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Cofit
Protein
Homologs
Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yaaJ and talB are separated by 278 nucleotides
talB and mog are separated by 114 nucleotides
mog and yaaH are separated by 34 nucleotides
yaaH and yaaW are separated by 148 nucleotides
b0007: yaaJ - predicted transporter (NCBI), at 6,529 to 7,959
yaaJ
b0008: talB - transaldolase B (NCBI), at 8,238 to 9,191
talB
b0009: mog - molybdenum cofactor biosynthesis protein (NCBI), at 9,306 to 9,893
mog
b0010: yaaH - conserved inner membrane protein associated with acetate transport (NCBI), at 9,928 to 10,494
yaaH
b0011: yaaW - hypothetical protein (NCBI), at 10,643 to 11,356
yaaW
Group
Condition
b0007
b0008
b0009
b0010
b0011
yaaJ
talB
mog
yaaH
yaaW
stress
2,4-Dinitrophenol 2 mM
-0.3
-1.3
-2.0
+0.0
-0.1
stress
2,4-Dinitrophenol 2 mM
-0.4
-1.2
-1.9
-0.1
-0.1
stress
LB Aerobic with Irgasan 0.0000003125 mM
-0.2
-1.1
-1.1
-0.1
-0.5
stress
octanol 0.08 vol%
+0.3
-1.0
-1.5
-0.1
-0.5
phage
Phage_N4_cage_3_mouse_1_Cecum_40 hr
-0.7
+0.1
-1.2
-1.0
+0.0
stress
LB Anaerobic Allura Red AC 250 uM
-0.7
-0.5
-1.2
-0.3
+0.0
phage
Phage_N4_cage_3_mice _Feces_40 hr
-0.4
-0.2
-1.3
-0.5
-0.2
phage
Phage_N4_cage_4_mouse_1_Cecum_40 hr
-0.2
-0.5
-1.1
-0.6
-0.1
predation
6 hr rep1_HD100_10fold_dilution_grewON
-0.5
-0.7
-0.8
-0.3
-0.2
control
PBS_cage_1_mouse_2_Cecum_40 hr
-0.3
+0.1
-1.3
-0.5
-0.3
phage
T7_phage 0.00015625 MOI TC4
-0.8
-0.9
-0.4
+0.4
-0.3
lb
LB, Anaerobic
-0.4
+0.1
-1.6
+0.3
-0.3
stress
LB Anaerobic with Nitrofurantoin 0.000015625 mM
+0.0
+0.6
-1.9
-0.3
+0.1
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+0.9
-0.9
-0.9
-0.4
-0.1
stress
Bacitracin 1 mg/ml
+0.4
-2.0
+0.3
-0.3
+0.2
stress
Benzalkonium Chloride 0.004 mg/ml
+0.6
-3.4
+2.1
+0.2
-0.7
stress
LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.3
-1.1
-0.8
+0.2
+0.2
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-0.1
-1.7
+0.6
+0.1
-0.0
stress
Spectinomycin 0.00625 mg/ml
+0.2
-1.8
+1.3
-0.6
-0.2
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+1.3
-0.6
-1.4
-0.1
-0.0
phage
Ste41
-0.2
-0.5
-0.5
+0.9
-0.3
stress
LB Anaerobic with Furaltadone 0.000015625 mM
+0.2
+0.8
-1.6
-0.4
+0.6
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.4
-0.6
+0.9
-0.5
-0.6
motility
outer cut, LB soft agar motility assay
-0.4
-1.7
+2.3
+0.5
-0.7
stress
Spectinomycin 0.0125 mg/ml
+0.3
-2.7
+2.2
+0.3
-0.1
stress
Cisplatin 0.1 mg/ml
+0.5
-1.0
+0.9
+0.1
-0.4
motility
inner cut, LB soft agar motility assay
-0.2
-0.6
+0.9
+0.1
+0.9
motility
outer cut, LB soft agar motility assay
-0.3
-0.4
+2.1
-0.4
+0.1
stress
2-Furfuraldehyde 0.25 vol%
-0.1
+1.5
+0.8
-0.3
+0.2
stress
LB Aerobic with Nitrofurantoin 0.00003125 mM
-0.0
+2.6
+0.9
+0.5
+0.2
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