Fitness for 5 genes in Escherichia coli BW25113

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500 nt11403188 and 11545551 overlap by 28 nucleotides11545551 and 11687943 overlap by 28 nucleotides11687943 and ygcF are separated by 339 nucleotidesygcF and yqcG are separated by 138 nucleotides 11403188 - no description, at 2,902,402 to 2,902,429 11403188 11545551 - no description, at 2,902,402 to 2,902,429 11545551 11687943 - no description, at 2,902,402 to 2,902,429 11687943 b2777: ygcF - hypothetical protein (NCBI), at 2,902,769 to 2,903,440 ygcF b4682: yqcG - expressed protein (RefSeq), at 2,903,579 to 2,903,719 yqcG
Group Condition 11403188 11545551 11687943 b2777 b4682
ygcF yqcG
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. N.D. N.D. -0.7 N.D.
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM N.D. N.D. N.D. -0.6 N.D.
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM N.D. N.D. N.D. -0.5 N.D.
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM N.D. N.D. N.D. -0.5 N.D.
phage T7_phage 0.0015625 MOI TC2 N.D. N.D. N.D. -0.5 N.D.
control PBS_cage_1_mice _Feces_16 hr N.D. N.D. N.D. -0.5 N.D.
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM N.D. N.D. N.D. -0.5 N.D.
predation 6 hr rep1_HD100_10fold_dilution_grewON N.D. N.D. N.D. -0.4 N.D.
gfp_control GFP_control N.D. N.D. N.D. -0.4 N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. N.D. N.D. -0.4 N.D.
phage N4_phage 0.009375 MOI TC2 N.D. N.D. N.D. -0.4 N.D.
phage Papa dilution 10-1 cacl2 maltose N.D. N.D. N.D. -0.4 N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. N.D. N.D. -0.4 N.D.
stress Ciprofloxacin Hydrochloride 0.000156 mM N.D. N.D. N.D. -0.4 N.D.
stress Cisplatin 0.1 mg/ml N.D. N.D. N.D. -0.4 N.D.
retron_ec_48_control retron_Ec_48_control N.D. N.D. N.D. -0.4 N.D.
control PBS_cage_1_mice _Feces_22 hr N.D. N.D. N.D. -0.3 N.D.
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. N.D. N.D. -0.3 N.D.
carbon source carbon source D-Glucose 20mM N.D. N.D. N.D. -0.3 N.D.
no stress control anaerobic M9 no stress control; Anaerobic 24 hr N.D. N.D. N.D. -0.3 N.D.
nophagecontrol R1_N4_T1_phage0 N.D. N.D. N.D. -0.3 N.D.
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=None N.D. N.D. N.D. +0.3 N.D.
stress nitrite 20 mM N.D. N.D. N.D. +0.3 N.D.
stress LB Anaerobic with Trimethoprim 0.00000125 mM N.D. N.D. N.D. +0.4 N.D.
stress LB Aerobic with Trimethoprim 0.00000125 mM N.D. N.D. N.D. +0.4 N.D.
phage Ste41 N.D. N.D. N.D. +0.4 N.D.
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB N.D. N.D. N.D. +0.4 N.D.
phage Ste41 N.D. N.D. N.D. +0.5 N.D.
stress Trimethoprim 0.0025 mM N.D. N.D. N.D. +0.5 N.D.
phage T7_phage 0.00015625 MOI TC4 N.D. N.D. N.D. +0.6 N.D.
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