Gene info for PS417_28100 in Pseudomonas simiae WCS417

Updated annotation (from data): glucose-6-phosphate 1-epimerase [EC: 5.1.3.15]
Rationale: Specifically important for: D-Trehalose dihydrate; N-Acetyl-D-Glucosamine. NAG is phosphorylated by a PTS system, deacetylated (PS417_22985), and converted to fructose-6-phosphate by an aminotranferase (PS417_22990). One might expect the fructose-6-P to be converted to fructose-1,6-bisphosphate, but no gene for that is apparent in this organism or many other Pseudomonas (nor did the fitness data highlight any kinase). Instead, the fructose-6-phosphate is apparently converted to glucose-6-phosphate by the glucose-6-phosphate isomerase (PS417_23920) and oxidized by G6P dehydrogenase (PS417_22110). (Both of these genes are essential so we cannot confirm this.) G6P dehydrogenase is reportedly specific for the beta form, while G6P isomerase produces the alpha form of G6P, so it makes sense for a glucose-6-P epimerase to be involved (even though the reaction can occur spontaneously). This gene is also mildly important for trehalose utilization, which involves the hydrolysis of trehalose 6-phosphate to glucose-6-phosphate. Because trehalose has an alpha 1->1 linkage, the product is probably alpha-G6P. Thus, this gene's phenotypes are consistent with KEGG's annotation as glucose-6-phosphate 1-epimerase. (KEGG_correct)
Original annotation: aldose epimerase
Type 1: Protein-coding gene
Located on scaffold CP007637, + strand, nucleotides 6,064,890 to 6,065,789
See nucleotide sequence around PS417_28100 or for all of scaffold CP007637

Other databases: NCBI, IMG: 2586034194, uniprot: A0A1N7UQW8

Nearby Genes

500 ntPS417_28075 and PS417_28080 are separated by 15 nucleotidesPS417_28080 and PS417_28085 are separated by 132 nucleotidesPS417_28085 and PS417_28090 are separated by 179 nucleotidesPS417_28090 and PS417_28095 are separated by 454 nucleotidesPS417_28095 and PS417_28100 are separated by 58 nucleotidesPS417_28100 and PS417_28105 are separated by 66 nucleotidesPS417_28105 and PS417_28110 are separated by 77 nucleotidesPS417_28110 and PS417_28115 are separated by 104 nucleotidesPS417_28115 and PS417_28120 are separated by 11 nucleotidesPS417_28120 and PS417_28125 are separated by 333 nucleotides PS417_28075: PS417_28075 - phosphate ABC transporter permease, at 6,057,413 to 6,059,083 _28075 PS417_28080: PS417_28080 - phosphate ABC transporter permease, at 6,059,099 to 6,061,384 _28080 PS417_28085: PS417_28085 - phosphate-binding protein, at 6,061,517 to 6,062,503 _28085 PS417_28090: PS417_28090 - MFS transporter, at 6,062,683 to 6,063,972 _28090 PS417_28095: PS417_28095 - long-chain acyl-CoA thioester hydrolase, at 6,064,427 to 6,064,831 _28095 PS417_28100: PS417_28100 - glucose-6-phosphate 1-epimerase [EC: 5.1.3.15] (from data), at 6,064,890 to 6,065,789 _28100 PS417_28105: PS417_28105 - hypothetical protein, at 6,065,856 to 6,066,392 _28105 PS417_28110: PS417_28110 - transglycosylase, at 6,066,470 to 6,066,715 _28110 PS417_28115: PS417_28115 - phosphoribosylaminoimidazole carboxylase, at 6,066,820 to 6,067,905 _28115 PS417_28120: PS417_28120 - N5-carboxyaminoimidazole ribonucleotide mutase, at 6,067,917 to 6,068,408 _28120 PS417_28125: PS417_28125 - LysR family transcriptional regulator, at 6,068,742 to 6,069,650 _28125
Locus Name Description Strand Phenotype
PS417_28075 PS417_28075 phosphate ABC transporter permease - strong
PS417_28080 PS417_28080 phosphate ABC transporter permease - strong
PS417_28085 PS417_28085 phosphate-binding protein - strong
PS417_28090 PS417_28090 MFS transporter - insig.
PS417_28095 PS417_28095 long-chain acyl-CoA thioester hydrolase + no data
PS417_28100 PS417_28100 glucose-6-phosphate 1-epimerase [EC: 5.1.3.15] (from data) + strong
PS417_28105 PS417_28105 hypothetical protein - insig.
PS417_28110 PS417_28110 transglycosylase - sig.
PS417_28115 PS417_28115 phosphoribosylaminoimidazole carboxylase - no data
PS417_28120 PS417_28120 N5-carboxyaminoimidazole ribonucleotide mutase - no data
PS417_28125 PS417_28125 LysR family transcriptional regulator - no data

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