Gene info for PS417_20960 in Pseudomonas simiae WCS417

Updated annotation (from data): Nitrate-binding regulatory protein (nasS)
Rationale: This protein is specifically important for the utilization of nitrate as a nitrogen source, but this may be a polar effect. It is74% identical to the nasS/PA1786 protein from P. aeruginosa (PMID:22493305) and to nasS from Azotobacter vinelandii (PMID:8748040), which binds nitrate and suppresses the activity of the transcriptional activator nasT. It is also more distantly related to the periplasmic substrate-binding components of nitrate ABC transporters (i.e., 37% identity to the N terminal part of ntrC/sll1452 and 36% identity to ntrA/sll1450), hence the misannotation as a transporter.
Original annotation: nitrate transporter
Type 1: Protein-coding gene
Located on scaffold CP007637, + strand, nucleotides 4,559,755 to 4,560,981
See nucleotide sequence around PS417_20960 or for all of scaffold CP007637

Other databases: NCBI, IMG: 2586032786, uniprot: A0A1N7U2J4

Nearby Genes

500 ntPS417_20935 and PS417_20940 are separated by 84 nucleotidesPS417_20940 and PS417_20945 overlap by 8 nucleotidesPS417_20945 and PS417_20950 are separated by 155 nucleotidesPS417_20950 and PS417_20955 are separated by 15 nucleotidesPS417_20955 and PS417_20960 are separated by 251 nucleotidesPS417_20960 and PS417_20965 are separated by 13 nucleotidesPS417_20965 and PS417_20970 are separated by 157 nucleotidesPS417_20970 and PS417_20975 overlap by 1 nucleotidesPS417_20975 and PS417_20980 are separated by 26 nucleotidesPS417_20980 and PS417_20985 are separated by 60 nucleotides PS417_20935: PS417_20935 - dihydroorotate dehydrogenase, at 4,555,703 to 4,556,722 _20935 PS417_20940: PS417_20940 - invasion protein IagB, at 4,556,807 to 4,557,268 _20940 PS417_20945: PS417_20945 - transcriptional regulator, at 4,557,261 to 4,557,656 _20945 PS417_20950: PS417_20950 - hypothetical protein, at 4,557,812 to 4,558,036 _20950 PS417_20955: PS417_20955 - secretion protein, at 4,558,052 to 4,559,503 _20955 PS417_20960: PS417_20960 - Nitrate-binding regulatory protein (nasS) (from data), at 4,559,755 to 4,560,981 _20960 PS417_20965: PS417_20965 - histidine kinase, at 4,560,995 to 4,561,570 _20965 PS417_20970: PS417_20970 - MFS transporter, at 4,561,728 to 4,562,939 _20970 PS417_20975: PS417_20975 - protein kinase, at 4,562,939 to 4,564,609 _20975 PS417_20980: PS417_20980 - uroporphyrin-III methyltransferase, at 4,564,636 to 4,565,379 _20980 PS417_20985: PS417_20985 - porin, at 4,565,440 to 4,566,420 _20985
Locus Name Description Strand Phenotype
PS417_20935 PS417_20935 dihydroorotate dehydrogenase - no data
PS417_20940 PS417_20940 invasion protein IagB - insig.
PS417_20945 PS417_20945 transcriptional regulator - insig.
PS417_20950 PS417_20950 hypothetical protein - insig.
PS417_20955 PS417_20955 secretion protein - insig.
PS417_20960 PS417_20960 Nitrate-binding regulatory protein (nasS) (from data) + strong
PS417_20965 PS417_20965 histidine kinase + strong
PS417_20970 PS417_20970 MFS transporter + strong
PS417_20975 PS417_20975 protein kinase + sig.
PS417_20980 PS417_20980 uroporphyrin-III methyltransferase + strong
PS417_20985 PS417_20985 porin - strong

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