Experiment set9S538 for Escherichia coli BL21
Ox4
Group: phageMedia: LB_plus_SM_buffer + MOI 10
Culturing: BL21_ML4, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 09/11/2024
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 4 genes in this experiment
For phage MOI 10 in Escherichia coli BL21
For phage MOI 10 across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| CMP-N-acetylneuraminate Biosynthesis | 1 |
| D-Galacturonate and D-Glucuronate Utilization | 1 |
| KDO2-Lipid A biosynthesis | 1 |
| Sialic Acid Metabolism | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Pentose and glucuronate interconversions
- Starch and sucrose metabolism
- Nucleotide sugars metabolism
- Aminosugars metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| UDP-N-acetyl-α-D-mannosamine biosynthesis | 1 | 1 | 1 |
| pectin degradation I | 3 | 1 | 1 |
| CMP-N-acetylneuraminate biosynthesis II (bacteria) | 3 | 1 | 1 |
| pectin degradation II | 5 | 3 | 1 |
| CMP-N-acetylneuraminate biosynthesis I (eukaryotes) | 5 | 2 | 1 |
| superpathway of CMP-sialic acids biosynthesis | 15 | 3 | 1 |
| superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis | 24 | 7 | 1 |