Experiment set9S537 for Escherichia coli BL21

Ox4

Group: phage
Media: LB_plus_SM_buffer + MOI 10
Culturing: BL21_ML4, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 9/11/24
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 6 genes in this experiment

For phage MOI 10 in Escherichia coli BL21

For phage MOI 10 across organisms

SEED Subsystems

Subsystem	#Specific
CMP-N-acetylneuraminate Biosynthesis	1
D-Galacturonate and D-Glucuronate Utilization	1
KDO2-Lipid A biosynthesis	1
Lipid A-Ara4N pathway ( Polymyxin resistance )	1
Oxidative stress	1
Sialic Acid Metabolism	1
Teichuronic acid biosynthesis	1
Transport of Zinc	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nucleotide sugars metabolism
• Pentose and glucuronate interconversions
• Ascorbate and aldarate metabolism
• Starch and sucrose metabolism
• Aminosugars metabolism
• Fructose and mannose metabolism
• C21-Steroid hormone metabolism
• Glycine, serine and threonine metabolism
• Bisphenol A degradation
• Polyketide sugar unit biosynthesis
• Linoleic acid metabolism
• Tetrachloroethene degradation
• Benzoate degradation via CoA ligation
• Butanoate metabolism
• Retinol metabolism
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of type II polyketide products
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
UDP-N-acetyl-α-D-mannosaminouronate biosynthesis	1	1	1
UDP-N-acetyl-α-D-mannosamine biosynthesis	1	1	1
UDP-α-D-glucuronate biosynthesis (from UDP-glucose)	1	1	1
UDP-α-D-xylose biosynthesis	2	1	1
CMP-N-acetylneuraminate biosynthesis II (bacteria)	3	1	1
pectin degradation I	3	1	1
pectin degradation II	5	3	1
CMP-N-acetylneuraminate biosynthesis I (eukaryotes)	5	2	1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis	6	3	1
UDP-sugars interconversion	9	1	1
teichuronic acid biosynthesis (B. subtilis 168)	9	1	1
superpathway of enterobacterial common antigen biosynthesis	10	9	1
colanic acid building blocks biosynthesis	11	10	1
superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis	24	7	2
superpathway of CMP-sialic acids biosynthesis	15	3	1