Experiment set9S253 for Pseudomonas aeruginosa MRSN321

LB with Sodium nitrate 500 mM

Group: stress
Media: LB + Sodium nitrate (500 mM), pH=7
Culturing: Paeruginosa_MRSN321_ML2, 96 deep-well block, Aerobic, at 30 (C), shaken=700 rpm
By: Hans and Ryan on 1/27/26
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 19 genes in this experiment

For stress Sodium nitrate in Pseudomonas aeruginosa MRSN321

For stress Sodium nitrate across organisms

SEED Subsystems

Subsystem	#Specific
Peptidoglycan Biosynthesis	2
ABC transporter alkylphosphonate (TC 3.A.1.9.1)	1
Bacterial Cell Division	1
Bacterial Cytoskeleton	1
Cyanophycin Metabolism	1
Folate Biosynthesis	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
Lipid A-Ara4N pathway ( Polymyxin resistance )	1
Molybdenum cofactor biosynthesis	1
Pterin biosynthesis	1
Queuosine-Archaeosine Biosynthesis	1
Siderophore Pyoverdine	1
Type IV pilus	1
YbbK	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Peptidoglycan biosynthesis
• Folate biosynthesis
• Purine metabolism
• Alanine and aspartate metabolism
• Lysine degradation
• Lipopolysaccharide biosynthesis
• Biotin metabolism
• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-asparagine biosynthesis I	1	1	1
L-glutamine degradation I	1	1	1
L-glutamate biosynthesis I	2	2	1
superpathway of L-asparagine biosynthesis	2	1	1
ammonia assimilation cycle III	3	3	1
6-hydroxymethyl-dihydropterin diphosphate biosynthesis IV (Plasmodium)	3	2	1
L-asparagine biosynthesis III (tRNA-dependent)	4	4	1
tetrahydromonapterin biosynthesis	4	3	1
glutaminyl-tRNAgln biosynthesis via transamidation	4	3	1
threo-tetrahydrobiopterin biosynthesis	4	2	1
erythro-tetrahydrobiopterin biosynthesis I	4	2	1
preQ0 biosynthesis	4	2	1
peptidoglycan maturation (meso-diaminopimelate containing)	12	4	3
6-hydroxymethyl-dihydropterin diphosphate biosynthesis I	5	4	1
peptidoglycan biosynthesis II (staphylococci)	17	12	3
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	3
L-glutamate and L-glutamine biosynthesis	7	6	1
drosopterin and aurodrosopterin biosynthesis	7	5	1
L-citrulline biosynthesis	8	6	1
peptidoglycan biosynthesis V (β-lactam resistance)	17	11	2
superpathway of tetrahydrofolate biosynthesis	10	9	1
peptidoglycan biosynthesis I (meso-diaminopimelate containing)	12	12	1
superpathway of tetrahydrofolate biosynthesis and salvage	12	11	1
superpathway of L-citrulline metabolism	12	8	1
peptidoglycan biosynthesis III (mycobacteria)	15	11	1
superpathway of chorismate metabolism	59	45	1