Experiment set9IT068 for Escherichia coli BW25113

LB Anaerobic with Chloramphenicol 0.000001953125 mM

Group: stress
Media: LB + Chloramphenicol (1.95E-03 mM)
Culturing: Keio_ML9a, 96 deep-well microplate; Multitron, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans_Hualan on 7/20/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 22 genes in this experiment

For stress Chloramphenicol in Escherichia coli BW25113

For stress Chloramphenicol across organisms

SEED Subsystems

Subsystem	#Specific
Transport of Nickel and Cobalt	5
Hydrogenases	4
Membrane-bound Ni, Fe-hydrogenase	3
Molybdenum cofactor biosynthesis	2
NiFe hydrogenase maturation	2
Anaerobic respiratory reductases	1
Glutathione-regulated potassium-efflux system and associated functions	1
Potassium homeostasis	1
Ribosome biogenesis bacterial	1
Transport of Molybdenum	1
Trehalose Uptake and Utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pyrimidine metabolism
• Glutathione metabolism
• Trinitrotoluene degradation

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
hydrogen oxidation I (aerobic)	1	1	1
NiFe(CO)(CN)2 cofactor biosynthesis	10	9	6
pseudouridine degradation	2	2	1
glutathione degradation (DUG pathway)	2	2	1
molybdopterin biosynthesis	6	6	1
γ-glutamyl cycle	6	5	1