Experiment set9IT060 for Escherichia coli BW25113

LB Anaerobic with Chlorhexidine diacetate salt hydrate 0.000000625 mM

Group: stress
Media: LB + Chlorhexidine diacetate salt hydrate (6.25E-04 mM)
Culturing: Keio_ML9a, 96 deep-well microplate; Multitron, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans_Hualan on 7/20/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 17 genes in this experiment

For stress Chlorhexidine diacetate salt hydrate in Escherichia coli BW25113

For stress Chlorhexidine diacetate salt hydrate across organisms

SEED Subsystems

Subsystem	#Specific
Proteasome bacterial	2
Proteolysis in bacteria, ATP-dependent	2
Beta-Glucoside Metabolism	1
Glycerol and Glycerol-3-phosphate Uptake and Utilization	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
Multidrug Resistance Efflux Pumps	1
Respiratory dehydrogenases 1	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Porphyrin and chlorophyll metabolism
• Phenylpropanoid biosynthesis
• Ubiquinone and menaquinone biosynthesis
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Cyanoamino acid metabolism
• Starch and sucrose metabolism
• Glycerolipid metabolism
• Glycerophospholipid metabolism
• Naphthalene and anthracene degradation
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
neolinustatin bioactivation	3	2	2
siroheme biosynthesis	4	4	2
glycerol-3-phosphate to hydrogen peroxide electron transport	2	2	1
glycerophosphodiester degradation	2	2	1
glycerol-3-phosphate to fumarate electron transfer	2	2	1
nitrate reduction IX (dissimilatory)	2	2	1
glycerol-3-phosphate shuttle	2	2	1
linustatin bioactivation	4	2	2
lotaustralin degradation	2	1	1
glycerol 3-phosphate to cytochrome aa3 oxidase electron transfer	2	1	1
glycerol-3-phosphate to cytochrome bo oxidase electron transfer	2	1	1
linamarin degradation	2	1	1
sn-glycerol 3-phosphate anaerobic respiration	3	3	1
glycerol degradation I	3	3	1
cellulose degradation II (fungi)	3	2	1
factor 430 biosynthesis	7	3	2
glycerol and glycerophosphodiester degradation	4	4	1
coumarin biosynthesis (via 2-coumarate)	5	2	1
α-tomatine degradation	6	1	1
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation)	13	2	2
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion)	15	3	2
firefly bioluminescence	14	2	1
adenosylcobalamin biosynthesis II (aerobic)	33	17	2
adenosylcobalamin biosynthesis I (anaerobic)	36	16	2