Experiment set9IT033 for Pseudomonas putida KT2440
LB 0.1x with 55.5 mM D-Glucose; pH 4.6
Group: pHMedia: LB (0.1x) + D-Glucose (55.5 mM), pH=4.6
Culturing: Putida_ML5_JBEI, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=250 rpm
By: Robin Herbert on 7/11/18
Media components: 1 g/L Tryptone, 0.5 g/L Yeast Extract, 0.5 g/L Sodium Chloride (final concentrations)
Specific Phenotypes
For 7 genes in this experiment
For pH D-Glucose in Pseudomonas putida KT2440
For pH D-Glucose across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Phosphate metabolism | 1 |
| Purine conversions | 1 |
| Pyruvate Alanine Serine Interconversions | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Purine metabolism
- Puromycin biosynthesis
- Alanine and aspartate metabolism
- Methionine metabolism
- Valine, leucine and isoleucine degradation
- Lysine biosynthesis
- Lysine degradation
- Arginine and proline metabolism
- beta-Alanine metabolism
- Aminophosphonate metabolism
- Nucleotide sugars metabolism
- Lipopolysaccharide biosynthesis
- Propanoate metabolism
- Caprolactam degradation
- Drug metabolism - other enzymes
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| adenosine nucleotides degradation III | 1 | 1 | 1 |
| putrescine degradation V | 2 | 2 | 1 |
| β-alanine degradation II | 2 | 2 | 1 |
| β-alanine biosynthesis II | 6 | 5 | 1 |
| superpathway of coenzyme A biosynthesis II (plants) | 10 | 9 | 1 |