Experiment set9IT015 for Escherichia coli BW25113

Compare to:

LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM

200 most detrimental genes:

  gene name fitness t score description  
b0142 folK +1.8 1.2 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b1865 nudB +1.7 2.4 dATP pyrophosphohydrolase (NCBI) compare
b3860 dsbA +1.6 10.8 periplasmic protein disulfide isomerase I (NCBI) compare
b1476 fdnI +1.5 1.4 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b2683 ygaH +1.4 6.2 predicted inner membrane protein (NCBI) compare
b1185 dsbB +1.4 5.0 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b2684 mprA +1.3 5.0 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b1696 ydiP +1.3 2.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b4551 yheV +1.1 3.0 hypothetical protein (NCBI) compare
b4438 cyaR +1.0 1.8 sRNA effector of ompX mRNA instability, cAMP-induced; hfq-dependent (RefSeq) compare
b1754 b1754 +1.0 1.9 orf, hypothetical protein (VIMSS) compare
b1370 insH-5 +1.0 1.5 Rac prophage; IS5 transposase and trans-activator (NCBI) compare
b4436 ryeC +1.0 0.9 Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq) compare
b2911 ssrS +1.0 1.7 6S regulatory RNA (NCBI) compare
b4416 rybA +0.9 1.6 unknown RNA (NCBI) compare
b2293 yfbT +0.9 2.6 putative phosphatase (VIMSS) compare
b2156 lysP +0.9 6.5 lysine transporter (NCBI) compare
b0784 moaD +0.9 2.3 molybdopterin synthase, small subunit (NCBI) compare
b4426 isrA +0.9 1.5 unknown RNA (NCBI) compare
b1251 yciI +0.9 2.9 orf, hypothetical protein (VIMSS) compare
b4394 yjjX +0.9 2.8 orf, hypothetical protein (VIMSS) compare
b3181 greA +0.9 3.5 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b2531 iscR +0.9 1.2 DNA-binding transcriptional repressor (NCBI) compare
b1562 hokD +0.8 2.2 Qin prophage; small toxic polypeptide (NCBI) compare
b1138 ymfE +0.8 1.4 e14 prophage; predicted inner membrane protein (NCBI) compare
b1842 holE +0.8 1.7 DNA polymerase III, theta subunit (NCBI) compare
b1920 fliY +0.8 1.9 cystine transporter subunit (NCBI) compare
b2808 gcvA +0.8 2.8 DNA-binding transcriptional dual regulator (NCBI) compare
b2080 b2080 +0.8 1.5 orf, hypothetical protein (VIMSS) compare
b4603 rseX +0.8 0.5 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b1263 trpD +0.8 1.7 bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI) compare
b1143 ymfI +0.8 1.8 orf, hypothetical protein (VIMSS) compare
b3183 obgE +0.8 1.0 GTPase involved in cell partioning and DNA repair (NCBI) compare
b0817 mntR +0.8 3.1 DNA-binding transcriptional regulator of mntH (NCBI) compare
b3257 yhdT +0.7 2.3 conserved inner membrane protein (NCBI) compare
b4533 ynfO +0.7 1.5 Qin prophage; predicted protein (NCBI) compare
b2433 b2433 +0.7 1.6 orf, hypothetical protein (VIMSS) compare
b4622 ytcA +0.7 3.0 hypothetical protein (RefSeq) compare
b1075 flgD +0.7 2.1 flagellar basal body rod modification protein D (NCBI) compare
b1558 cspF +0.7 1.4 Qin prophage; cold shock protein (NCBI) compare
b2682 ygaZ +0.7 2.9 predicted transporter (NCBI) compare
b0880 cspD +0.7 1.5 cold shock protein homolog (NCBI) compare
b4598 yncL +0.7 1.6 hypothetical protein (RefSeq) compare
b1231 tyrT +0.7 0.7 tRNA-Tyr (NCBI) compare
b1147 ymfL +0.7 1.7 e14 prophage; predicted DNA-binding transcriptional regulator (RefSeq) compare
b1647 ydhF +0.7 2.0 predicted oxidoreductase (NCBI) compare
b1516 lsrB +0.6 2.2 AI2 transporter (NCBI) compare
b1080 flgI +0.6 3.9 homolog of Salmonella P-ring of flagella basal body (VIMSS) compare
b0580 ybdJ +0.6 1.5 predicted inner membrane protein (NCBI) compare
b4631 ykgQ +0.6 1.1 no description compare
b4406 yaeP +0.6 1.7 hypothetical protein (NCBI) compare
b1563 relE +0.6 1.2 Qin prophage; toxin of the RelE-RelB toxin-antitoxin system (NCBI) compare
b2904 gcvH +0.6 1.6 glycine cleavage system protein H (NCBI) compare
b4451 ryhB +0.6 0.9 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b1627 rsxA +0.6 1.9 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b1566 flxA +0.6 1.8 Qin prophage; predicted protein (NCBI) compare
b1879 flhA +0.6 3.8 flagellar biosynthesis protein A (NCBI) compare
b2480 bcp +0.6 2.6 thioredoxin-dependent thiol peroxidase (NCBI) compare
b1728 ydjM +0.6 1.6 predicted inner membrane protein regulated by LexA (RefSeq) compare
b3250 mreC +0.6 1.2 cell wall structural complex MreBCD transmembrane component MreC (NCBI) compare
b1751 ydjY +0.6 1.7 orf, hypothetical protein (VIMSS) compare
b0840 deoR +0.6 2.0 DNA-binding transcriptional repressor (NCBI) compare
b4457 csrC +0.6 1.9 regulatory RNA (NCBI) compare
b2674 nrdI +0.6 2.2 hypothetical protein (NCBI) compare
b2912 ygfA +0.6 1.7 putative ligase (VIMSS) compare
b4030 yjbA +0.5 2.5 phosphate-starvation-inducible protein PsiE (NCBI) compare
b2025 hisF +0.5 2.1 imidazole glycerol phosphate synthase subunit HisF (NCBI) compare
b1950 fliR +0.5 1.1 flagellar biosynthesis protein R (NCBI) compare
b1809 b1809 +0.5 3.0 orf, hypothetical protein (VIMSS) compare
b2060 b2060 +0.5 2.9 orf, hypothetical protein (VIMSS) compare
b2059 wcaA +0.5 2.2 predicted glycosyl transferase (NCBI) compare
b4633 xisD +0.5 2.0 no description compare
b0348 mhpB +0.5 2.8 2,3-dihydroxyphenylpropionate 1,2-dioxygenase (NCBI) compare
b4373 rimI +0.5 1.7 ribosomal-protein-alanine N-acetyltransferase (NCBI) compare
b1020 phoH +0.5 3.0 conserved protein with nucleoside triphosphate hydrolase domain (NCBI) compare
b1618 uidR +0.5 1.9 DNA-binding transcriptional repressor (NCBI) compare
b4585 sroB +0.5 0.7 Novel sRNA, function unknown (RefSeq) compare
b3101 yqjF +0.5 1.9 orf, hypothetical protein (VIMSS) compare
b2125 yehT +0.5 2.2 orf, hypothetical protein (VIMSS) compare
b2281 nuoI +0.5 1.5 NADH dehydrogenase subunit I (NCBI) compare
b0872 hcr +0.5 2.1 HCP oxidoreductase, NADH-dependent (NCBI) compare
b0597 ybdB +0.5 2.1 hypothetical protein (NCBI) compare
b1480 sra +0.5 0.5 30S ribosomal subunit protein S22 (NCBI) compare
b1953 b1953 +0.5 1.6 orf, hypothetical protein (VIMSS) compare
b1397 paaJ +0.5 2.6 acetyl-CoA acetyltransferase (NCBI) compare
b3239 yhcO +0.5 1.8 predicted barnase inhibitor (NCBI) compare
b2726 hypA +0.5 1.7 protein involved in nickel insertion into hydrogenases 3 (NCBI) compare
b4647 mokA +0.5 0.4 no description compare
b1670 ydhU +0.5 2.0 predicted cytochrome (NCBI) compare
b1514 lsrC +0.5 1.1 AI2 transporter (NCBI) compare
b0442 ybaV +0.5 2.6 hypothetical protein (NCBI) compare
b2688 gshA +0.5 2.8 glutamate--cysteine ligase (NCBI) compare
b1388 paaA +0.5 1.8 predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI) compare
b1261 trpB +0.5 1.3 tryptophan synthase subunit beta (NCBI) compare
b4441 glmY +0.5 1.1 sRNA activator of glmS mRNA (RefSeq) compare
b3169 nusA +0.5 1.5 transcription elongation factor NusA (NCBI) compare
b1984 asnW +0.5 0.6 tRNA-Asn (NCBI) compare
b2705 srlD +0.5 2.6 3-ketoacyl-(acyl-carrier-protein) reductase (NCBI) compare
b1221 narL +0.5 1.6 DNA-binding response regulator in two-component regulatory system with NarX (or NarQ) (NCBI) compare
b1832 yebR +0.5 2.1 free methionine-(R)-sulfoxide reductase (RefSeq) compare
b1724 ydiZ +0.5 1.0 hypothetical protein (NCBI) compare
b1079 flgH +0.5 1.4 flagellar L-ring protein precursor H (NCBI) compare
b2300 yfcE +0.5 1.8 orf, hypothetical protein (VIMSS) compare
b4370 leuQ +0.5 0.9 tRNA-Leu (NCBI) compare
b1688 ydiK +0.5 2.3 predicted inner membrane protein (NCBI) compare
b1991 cobT +0.5 2.2 nicotinate-nucleotide--dimethylbenzimidazolephosphoribosyltransferase (NCBI) compare
b1399 paaX +0.5 1.8 DNA-binding transcriptional repressor of phenylacetic acid degradation, aryl-CoA responsive (NCBI) compare
b2499 purM +0.5 1.5 phosphoribosylaminoimidazole synthetase (NCBI) compare
b4588 ylcH +0.5 1.7 hypothetical protein, DLP12 prophage (RefSeq) compare
b0850 ybjC +0.5 1.4 predicted inner membrane protein (NCBI) compare
b1941 fliI +0.5 2.7 flagellum-specific ATP synthase (NCBI) compare
b2782 chpA +0.5 1.9 toxin of the ChpA-ChpR toxin-antitoxin system, endoribonuclease (NCBI) compare
b1315 ycjS +0.5 2.1 predicted oxidoreductase, NADH-binding (NCBI) compare
b1519 tam +0.4 1.4 trans-aconitate 2-methyltransferase (NCBI) compare
b2980 glcC +0.4 2.4 DNA-binding transcriptional dual regulator, glycolate-binding (NCBI) compare
b2637 yfjT +0.4 1.3 CP4-57 prophage; predicted protein (NCBI) compare
b1072 flgA +0.4 2.0 flagellar basal body P-ring biosynthesis protein A (NCBI) compare
b4217 ytfK +0.4 2.3 orf, hypothetical protein (VIMSS) compare
b4527 b1354 +0.4 1.7 orf, hypothetical protein (VIMSS) compare
b4450 arcZ +0.4 1.6 sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq) compare
b1219 ychN +0.4 1.0 hypothetical protein (NCBI) compare
b4353 yjiX +0.4 2.3 hypothetical protein (NCBI) compare
b1844 exoX +0.4 1.9 exodeoxyribonuclease X (NCBI) compare
b1939 fliG +0.4 1.8 flagellar motor switch protein G (NCBI) compare
b4388 serB +0.4 2.0 3-phosphoserine phosphatase (NCBI) compare
b2230 yfaA +0.4 3.0 orf, hypothetical protein (VIMSS) compare
b2027 wzzB +0.4 2.0 regulator of length of O-antigen component of lipopolysaccharide chains (VIMSS) compare
b4573 insZ +0.4 1.6 no description compare
b0828 iaaA +0.4 1.3 L-asparaginase (NCBI) compare
b2457 cchA +0.4 1.4 detox protein (VIMSS) compare
b1669 ydhT +0.4 1.1 hypothetical protein (NCBI) compare
b2846 yqeH +0.4 0.2 orf, hypothetical protein (VIMSS) compare
b2259 pmrD +0.4 0.6 polymyxin resistance protein B (VIMSS) compare
b1077 flgF +0.4 2.5 flagellar component of cell-proximal portion of basal-body rod (NCBI) compare
b0219 yafV +0.4 1.5 predicted C-N hydrolase family amidase, NAD(P)-binding (NCBI) compare
b2810 csdA +0.4 2.2 cysteine sulfinate desulfinase (NCBI) compare
b3107 yhaL +0.4 0.7 orf, hypothetical protein (VIMSS) compare
b4458 oxyS +0.4 0.6 global regulatory RNA (NCBI) compare
b3766 ilvL +0.4 1.4 ilvG operon leader peptide (NCBI) compare
b1679 sufE +0.4 1.3 cysteine desufuration protein SufE (NCBI) compare
b1594 dgsA +0.4 1.2 DNA-binding transcriptional repressor (NCBI) compare
b0487 cueR +0.4 1.4 DNA-binding transcriptional activator of copper-responsive regulon genes (NCBI) compare
b1569 dicC +0.4 1.4 Qin prophage; DNA-binding transcriptional regulator for DicB (NCBI) compare
b0810 glnP +0.4 1.5 glutamine ABC transporter permease protein (NCBI) compare
b4414 tff +0.4 1.4 unknown RNA (NCBI) compare
b1943 fliK +0.4 2.1 flagellar hook-length control protein (NCBI) compare
b2211 yojI +0.4 2.8 fused predicted multidrug transport subunits of ABC superfamily: membrane component/ATP-binding component (NCBI) compare
b1752 ydjZ +0.4 1.0 conserved inner membrane protein (NCBI) compare
b2600 tyrA +0.4 2.3 fused chorismate mutase T/prephenate dehydrogenase (NCBI) compare
b1640 ydhH +0.4 1.6 anhydro-N-acetylmuramic acid kinase (NCBI) compare
b1311 ycjO +0.4 2.5 predicted sugar transporter subunit: membrane component of ABC superfamily (NCBI) compare
b0854 potF +0.4 1.4 putrescine transporter subunit: periplasmic-binding component of ABC superfamily (NCBI) compare
b1341 b1341 +0.4 1.9 orf, hypothetical protein (VIMSS) compare
b2047 wcaJ +0.4 1.3 predicted UDP-glucose lipid carrier transferase (NCBI) compare
b1358 ydaT +0.4 0.6 Rac prophage; predicted protein (NCBI) compare
b1310 ycjN +0.4 1.7 predicted sugar transporter subunit: periplasmic-binding component of ABC superfamily (NCBI) compare
b3858 yihD +0.4 0.8 hypothetical protein (NCBI) compare
b1063 yceB +0.4 1.2 predicted lipoprotein (NCBI) compare
b0979 appB +0.4 1.4 cytochrome bd-II oxidase, subunit II (NCBI) compare
b1244 oppB +0.4 2.3 oligopeptide permease ABC transporter membrane protein (NCBI) compare
b2169 fruB +0.4 2.1 fused fructose-specific PTS enzymes: IIA component/HPr component (NCBI) compare
b0752 zitB +0.4 2.1 zinc transporter ZitB (NCBI) compare
b0987 ymcD +0.4 2.1 orf, hypothetical protein (VIMSS) compare
b4369 leuP +0.4 1.1 tRNA-Leu (NCBI) compare
b2969 yghE +0.4 1.3 predicted secretion pathway protein, L-type protein (pseudogene) (RefSeq) compare
b4053 alr +0.4 2.7 alanine racemase (NCBI) compare
b0502 ylbG +0.4 1.2 no description compare
b1061 dinI +0.4 1.5 DNA damage-inducible protein I (NCBI) compare
b1442 ydcU +0.4 1.7 predicted spermidine/putrescine transporter subunit (NCBI) compare
b1530 marR +0.4 0.9 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b2432 yfeY +0.4 0.9 hypothetical protein (NCBI) compare
b1191 b1191 +0.4 2.3 orf, hypothetical protein (VIMSS) compare
b4521 ycgI +0.4 1.1 hypothetical protein (NCBI) compare
b0520 ylbF +0.4 1.8 hypothetical protein (NCBI) compare
b1572 ydfB +0.4 0.7 orf, hypothetical protein (VIMSS) compare
b0398 sbcD +0.4 1.7 exonuclease, dsDNA, ATP-dependent (NCBI) compare
b1372 b1372 +0.4 1.8 putative membrane protein (VIMSS) compare
b0796 ybiH +0.4 1.0 putative transcriptional regulator (VIMSS) compare
b1110 ycfJ +0.4 1.9 hypothetical protein (NCBI) compare
b3636 rpmG +0.4 0.6 50S ribosomal protein L33 (NCBI) compare
b2071 yegJ +0.4 0.4 hypothetical protein (NCBI) compare
b0378 yaiW +0.4 2.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b0735 ybgE +0.4 1.5 conserved inner membrane protein (NCBI) compare
b2365 dsdX +0.4 2.6 predicted transporter (NCBI) compare
b2092 gatC +0.4 2.0 galactitol-specific enzyme IIC component of PTS (NCBI) compare
b1287 yciW +0.4 1.4 putative oxidoreductase (VIMSS) compare
b1806 yeaY +0.4 0.9 predicted lipoprotein (NCBI) compare
b0845 ybjJ +0.4 2.1 predicted transporter (NCBI) compare
b0220 ivy +0.4 2.0 inhibitor of vertebrate C-lysozyme (NCBI) compare
b1153 ymfQ +0.4 0.8 e14 prophage; conserved protein (NCBI) compare
b0756 galM +0.4 1.7 galactose-1-epimerase (mutarotase) (NCBI) compare
b2903 gcvP +0.4 3.0 glycine dehydrogenase (NCBI) compare
b0778 bioD +0.4 1.3 dethiobiotin synthetase (NCBI) compare
b1332 ynaJ +0.4 0.2 predicted inner membrane protein (NCBI) compare
b0249 ykfF +0.4 0.9 CP4-6 prophage; predicted protein (NCBI) compare
b0120 speD +0.4 2.4 S-adenosylmethionine decarboxylase proenzyme (NCBI) compare
b0858 ybjO +0.4 0.5 predicted inner membrane protein (NCBI) compare
b2920 ygfH +0.4 1.8 propionyl-CoA:succinate-CoA transferase (NCBI) compare
b4137 cutA +0.4 2.3 copper binding protein, copper sensitivity (NCBI) compare
b1427 rimL +0.4 0.9 ribosomal-protein-L7/L12-serine acetyltransferase (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress 8-hydroxy-nitroquinoline across organisms