Experiment set8S751 for Pseudomonas aeruginosa MRSN321

PA14_MA4_d

Group: phage
Media: LB
Culturing: Paeruginosa_MRSN321_ML2, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: DP on 9/4/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 10 genes in this experiment

SEED Subsystems

Subsystem	#Specific
Experimental tye	1
Flagellar motility	1
Heme and Siroheme Biosynthesis	1
Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization	1
Thiamin biosynthesis	1
Ton and Tol transport systems	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fatty acid biosynthesis
• Ubiquinone and menaquinone biosynthesis
• alpha-Linolenic acid metabolism
• Benzoate degradation via CoA ligation
• Thiamine metabolism
• Porphyrin and chlorophyll metabolism
• Limonene and pinene degradation
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
glyphosate degradation II	1	1	1
heme b biosynthesis II (oxygen-independent)	4	3	1
superpathway of heme b biosynthesis from uroporphyrinogen-III	6	4	1
thiazole component of thiamine diphosphate biosynthesis II	7	5	1
butanol and isobutanol biosynthesis (engineered)	8	3	1
3,8-divinyl-chlorophyllide a biosynthesis II (anaerobic)	9	3	1
superpathway of thiamine diphosphate biosynthesis II	11	9	1
superpathway of bacteriochlorophyll a biosynthesis	26	6	1