Experiment set8S184 for Escherichia coli ECOR27

N4

Group: phage
Media: LB_plus_SM_buffer + Phage (10 MOI)
Culturing: Ecoli_ECOR27_ML1, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 12/17/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 13 genes in this experiment

For phage Phage in Escherichia coli ECOR27

For phage Phage across organisms

SEED Subsystems

Subsystem	#Specific
Adenosyl nucleosidases	1
Biogenesis of c-type cytochromes	1
Campylobacter Iron Metabolism	1
DNA-replication	1
DNA repair, UvrABC system	1
Deoxyribose and Deoxynucleoside Catabolism	1
Iron acquisition in Vibrio	1
Orphan regulatory proteins	1
Periplasmic disulfide interchange	1
Purine conversions	1
Ton and Tol transport systems	1
Transport of Iron	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Pyrimidine metabolism
• Glycine, serine and threonine metabolism
• Cysteine metabolism
• Lipopolysaccharide biosynthesis
• Nicotinate and nicotinamide metabolism
• Folate biosynthesis
• Biosynthesis of alkaloids derived from histidine and purine

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-serine degradation	3	3	3
purine deoxyribonucleosides degradation I	4	4	3
purine ribonucleosides degradation	6	6	4
L-tryptophan degradation II (via pyruvate)	3	3	2
purine deoxyribonucleosides degradation II	3	3	2
D-serine degradation	3	3	2
L-cysteine degradation II	3	2	2
adenine and adenosine salvage III	4	4	2
guanine and guanosine salvage I	2	2	1
periplasmic disulfide bond reduction	2	2	1
xanthine and xanthosine salvage	2	2	1
adenine and adenosine salvage I	2	2	1
superpathway of purine deoxyribonucleosides degradation	7	7	3
glycine betaine degradation III	7	4	3
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis	5	2	2
glycine betaine degradation I	8	4	3
pyrimidine deoxyribonucleosides degradation	3	3	1
glycine degradation	3	3	1
adenine and adenosine salvage V	3	3	1
superpathway of guanine and guanosine salvage	3	3	1
L-methionine biosynthesis II	6	5	2
purine nucleotides degradation II (aerobic)	11	11	3
inosine 5'-phosphate degradation	4	4	1
guanosine nucleotides degradation III	4	4	1
L-mimosine degradation	8	4	2
glutathione-mediated detoxification I	8	3	2
adenosine nucleotides degradation II	5	5	1
nucleoside and nucleotide degradation (archaea)	10	4	2
superpathway of pyrimidine deoxyribonucleosides degradation	6	6	1
nucleoside and nucleotide degradation (halobacteria)	6	3	1
fluoroacetate and fluorothreonine biosynthesis	6	1	1
superpathway of purine nucleotide salvage	14	13	2
ureide biosynthesis	7	5	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II	15	13	2
purine nucleobases degradation II (anaerobic)	24	17	3
cytochrome c biogenesis (system I type)	11	11	1
salinosporamide A biosynthesis	15	3	1
arsenic detoxification (mammals)	17	8	1