Experiment set8IT096 for Escherichia coli BW25113

Compare to:

LB Aerobic with Hygromycin B 0.000015625 mM

200 most important genes:

  gene name fitness t score description  
b0407 yajC -3.6 -3.5 preprotein translocase subunit YajC (NCBI) compare
b4174 hflK -3.4 -9.5 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b4175 hflC -3.4 -9.1 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b0113 pdhR -2.8 -4.8 transcriptional regulator of pyruvate dehydrogenase complex (NCBI) compare
b1493 gadB -2.6 -1.4 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b1346 ydaQ -2.5 -2.2 orf, hypothetical protein (VIMSS) compare
b1292 sapC -2.4 -1.6 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3912 cpxR -2.4 -8.7 DNA-binding response regulator in two-component regulatory system with CpxA (NCBI) compare
b1175 minD -2.3 -4.7 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b1882 cheY -2.2 -1.2 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b1861 ruvA -2.1 -1.4 Holliday junction DNA helicase motor protein (NCBI) compare
b2258 b2258 -2.1 -2.0 putative transport/receptor protein (VIMSS) compare
b1575 dicB -2.1 -2.2 Qin prophage; cell division inhibition protein (NCBI) compare
b0142 folK -2.0 -1.1 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b1176 minC -2.0 -3.6 septum formation inhibitor (NCBI) compare
b1290 sapF -2.0 -1.8 predicted antimicrobial peptide transporter subunit (NCBI) compare
b4685 yrbN -2.0 -2.3 expressed protein (RefSeq) compare
b2796 sdaC -2.0 -12.4 predicted serine transporter (NCBI) compare
b2501 ppk -1.9 -8.1 polyphosphate kinase (NCBI) compare
b1293 sapB -1.8 -1.6 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1860 ruvB -1.8 -1.2 Holliday junction DNA helicase B (NCBI) compare
b4163 glyV -1.8 -1.7 tRNA-Gly (NCBI) compare
b3931 hslU -1.8 -9.7 ATP-dependent protease ATP-binding subunit (NCBI) compare
b4701 sokX -1.8 -1.1 sok-related sRNA, function unknown (RefSeq) compare
b0738 tolR -1.7 -2.5 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b3932 hslV -1.6 -8.9 ATP-dependent protease peptidase subunit (NCBI) compare
b1863 ruvC -1.6 -2.0 Holliday junction resolvase (NCBI) compare
b0441 ppiD -1.6 -9.6 peptidyl-prolyl cis-trans isomerase (rotamase D) (NCBI) compare
b4529 ydbJ -1.6 -1.4 hypothetical protein (NCBI) compare
b1291 sapD -1.6 -1.4 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3781 trxA -1.6 -3.1 thioredoxin 1 (VIMSS) compare
b2830 nudH -1.5 -1.0 dinucleoside polyphosphate hydrolase (NCBI) compare
b4676 yoaK -1.5 -1.0 expressed protein (RefSeq) compare
b2259 pmrD -1.5 -1.4 polymyxin resistance protein B (VIMSS) compare
b3204 ptsN -1.5 -4.2 sugar-specific enzyme IIA component of PTS (NCBI) compare
b4171 miaA -1.5 -2.4 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b0737 tolQ -1.5 -1.8 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b3035 tolC -1.4 -6.5 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b3229 sspA -1.4 -1.8 stringent starvation protein A (NCBI) compare
b2156 lysP -1.4 -7.7 lysine transporter (NCBI) compare
b3726 pstA -1.3 -3.5 phosphate transporter subunit (NCBI) compare
b0989 cspH -1.3 -2.2 stress protein, member of the CspA-family (NCBI) compare
b4503 yafF -1.3 -0.8 no description compare
b1514 lsrC -1.3 -2.1 AI2 transporter (NCBI) compare
b0049 apaH -1.3 -1.8 diadenosinetetraphosphatase (NCBI) compare
b3792 wzxE -1.3 -4.6 O-antigen translocase (NCBI) compare
b2569 lepA -1.3 -2.5 GTP-binding protein LepA (NCBI) compare
b3611 yibN -1.3 -3.9 predicted rhodanese-related sulfurtransferase (NCBI) compare
b0741 pal -1.2 -2.6 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b3390 aroK -1.2 -1.5 shikimate kinase I (NCBI) compare
b3961 oxyR -1.2 -7.4 DNA-binding transcriptional dual regulator (NCBI) compare
b3617 kbl -1.2 -5.3 2-amino-3-ketobutyrate coenzyme A ligase (NCBI) compare
b2168 fruK -1.2 -1.6 1-phosphofructokinase (NCBI) compare
b1482 osmC -1.2 -2.0 osmotically inducible, stress-inducible membrane protein (NCBI) compare
b0053 surA -1.2 -3.5 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b3849 trkH -1.2 -6.9 potassium transporter (NCBI) compare
b3649 rpoZ -1.2 -2.2 DNA-directed RNA polymerase subunit omega (NCBI) compare
b1348 lar -1.2 -1.7 Rac prophage; restriction alleviation protein (NCBI) compare
b1671 ydhX -1.1 -2.5 predicted 4Fe-4S ferridoxin-type protein (RefSeq) compare
b3613 yibP -1.1 -1.1 putative membrane protein (VIMSS) compare
b3619 rfaD -1.1 -2.0 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b3466 yhhL -1.1 -3.2 conserved inner membrane protein (NCBI) compare
b3962 udhA -1.1 -4.1 putative oxidoreductase (VIMSS) compare
b2952 yggT -1.1 -3.6 predicted inner membrane protein (NCBI) compare
b1642 slyA -1.1 -1.1 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b0439 lon -1.1 -3.3 DNA-binding ATP-dependent protease La (NCBI) compare
b3026 qseC -1.0 -2.1 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b3162 deaD -1.0 -2.3 inducible ATP-independent RNA helicase (VIMSS) compare
b3973 birA -1.0 -2.3 biotin--protein ligase (NCBI) compare
b2518 ndk -1.0 -1.6 nucleoside diphosphate kinase (NCBI) compare
b3052 rfaE -1.0 -3.1 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b4481 rffT -1.0 -4.4 4-alpha-L-fucosyltransferase (NCBI) compare
b1506 yneL -1.0 -1.2 predicted transcriptional regulator (NCBI) compare
b4598 yncL -1.0 -2.1 hypothetical protein (RefSeq) compare
b0222 lpcA -1.0 -1.9 phosphoheptose isomerase (NCBI) compare
b4557 yidD -1.0 -2.6 hypothetical protein (NCBI) compare
b4406 yaeP -1.0 -2.0 hypothetical protein (NCBI) compare
b2808 gcvA -1.0 -2.6 DNA-binding transcriptional dual regulator (NCBI) compare
b1531 marA -1.0 -3.0 multiple antibiotic resistance; transcriptional activator of defense systems (VIMSS) compare
b3621 rfaC -1.0 -3.1 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b1418 cybB -1.0 -1.7 cytochrome b(561) (VIMSS) compare
b1739 osmE -1.0 -2.1 DNA-binding transcriptional activator (NCBI) compare
b0970 yccA -1.0 -2.3 inner membrane protein (NCBI) compare
b3405 ompR -1.0 -4.2 osmolarity response regulator (NCBI) compare
b2924 mscS -1.0 -6.2 mechanosensitive channel (NCBI) compare
b4702 mgtL -1.0 -1.0 regulatory leader peptide for mgtA (NCBI) compare
b3791 rffA -1.0 -4.7 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b3636 rpmG -0.9 -1.2 50S ribosomal protein L33 (NCBI) compare
b1380 ldhA -0.9 -1.5 D-lactate dehydrogenase (NCBI) compare
b4640 yoeG -0.9 -1.2 no description compare
b2506 yfgI -0.9 -1.6 hypothetical protein (NCBI) compare
b1164 ycgZ -0.9 -0.9 hypothetical protein (NCBI) compare
b1550 b1550 -0.9 -1.0 orf, hypothetical protein (VIMSS) compare
b2318 truA -0.9 -1.8 tRNA pseudouridine synthase A (NCBI) compare
b3977 tyrU -0.9 -0.8 tRNA-Tyr (NCBI) compare
b3933 ftsN -0.9 -5.1 essential cell division protein (NCBI) compare
b1505 ydeT -0.9 -0.8 hypothetical protein (RefSeq) compare
b2340 sixA -0.9 -2.7 phosphohistidine phosphatase (NCBI) compare
b2479 gcvR -0.9 -2.9 transcriptional regulation of gcv operon (VIMSS) compare
b1536 ydeI -0.9 -0.6 hypothetical protein (NCBI) compare
b4202 rpsR -0.9 -0.9 30S ribosomal protein S18 (NCBI) compare
b0888 trxB -0.9 -1.8 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare
b2350 yfdG -0.9 -4.0 CPS-53 (KpLE1) prophage; bactoprenol-linked glucose translocase (flippase) (NCBI) compare
b2181 yejG -0.9 -2.3 hypothetical protein (NCBI) compare
b2895 fldB -0.9 -1.8 flavodoxin 2 (NCBI) compare
b1579 intQ -0.9 -1.2 Qin prophage; predicted defective integrase (RefSeq) compare
b0623 cspE -0.9 -1.3 cold shock protein E (NCBI) compare
b0722 sdhD -0.9 -2.9 succinate dehydrogenase cytochrome b556 small membrane subunit (NCBI) compare
b0436 tig -0.9 -4.0 trigger factor (NCBI) compare
b0082 mraW -0.9 -1.9 S-adenosyl-methyltransferase (NCBI) compare
b1048 mdoG -0.9 -3.1 glucan biosynthesis protein, periplasmic (NCBI) compare
b3387 dam -0.9 -5.4 DNA adenine methylase (NCBI) compare
b1198 dhaM -0.9 -3.4 fused predicted dihydroxyacetone-specific PTS enzymes: HPr component/EI component (RefSeq) compare
b1103 ycfF -0.9 -1.1 orf, hypothetical protein (VIMSS) compare
b3616 tdh -0.9 -3.1 L-threonine 3-dehydrogenase (NCBI) compare
b0502 ylbG -0.9 -2.0 no description compare
b3778 rep -0.8 -3.4 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b3498 prlC -0.8 -5.8 oligopeptidase A (NCBI) compare
b0605 ahpC -0.8 -2.4 alkyl hydroperoxide reductase, C22 subunit (NCBI) compare
b1105 ycfM -0.8 -2.5 predicted outer membrane lipoprotein (NCBI) compare
b2576 srmB -0.8 -1.4 ATP-dependent RNA helicase (NCBI) compare
b1907 tyrP -0.8 -2.9 tyrosine transporter (NCBI) compare
b3839 tatC -0.8 -3.8 TatABCE protein translocation system subunit (NCBI) compare
b1485 ddpC -0.8 -2.2 D-ala-D-ala transporter subunit (NCBI) compare
b1730 ydjO -0.8 -0.5 predicted protein (RefSeq) compare
b1823 cspC -0.8 -1.5 stress protein, member of the CspA-family (NCBI) compare
b1951 rcsA -0.8 -0.5 DNA-binding transcriptional activator, co-regulator with RcsB (NCBI) compare
b1196 ycgY -0.8 -1.7 hypothetical protein (NCBI) compare
b4431 rprA -0.8 -1.4 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b1161 ycgX -0.8 -0.5 hypothetical protein (NCBI) compare
b3183 obgE -0.8 -0.8 GTPase involved in cell partioning and DNA repair (NCBI) compare
b4570 lomR -0.8 -1.4 no description compare
b0406 tgt -0.8 -3.6 queuine tRNA-ribosyltransferase (NCBI) compare
b1765 ydjA -0.8 -0.9 predicted oxidoreductase (NCBI) compare
b1147 ymfL -0.8 -1.6 e14 prophage; predicted DNA-binding transcriptional regulator (RefSeq) compare
b2198 ccmD -0.8 -1.7 cytochrome c biogenesis protein (NCBI) compare
b1502 ydeQ -0.8 -0.9 predicted fimbrial-like adhesin protein (NCBI) compare
b1249 cls -0.8 -3.4 cardiolipin synthetase (NCBI) compare
b4203 rplI -0.8 -2.3 50S ribosomal protein L9 (NCBI) compare
b2620 smpB -0.8 -0.5 SsrA-binding protein (NCBI) compare
b0564 appY -0.8 -0.6 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b1231 tyrT -0.8 -0.8 tRNA-Tyr (NCBI) compare
b1344 ydaO -0.8 -1.7 predicted C32 tRNA thiolase (NCBI) compare
b1762 b1762 -0.8 -1.5 orf, hypothetical protein (VIMSS) compare
b3790 rffC -0.8 -2.8 TDP-fucosamine acetyltransferase (RefSeq) compare
b1049 mdoH -0.8 -4.2 glucosyltransferase MdoH (NCBI) compare
b1258 yciF -0.8 -0.5 hypothetical protein (NCBI) compare
b1576 ydfD -0.8 -0.4 Qin prophage; predicted protein (NCBI) compare
b4436 ryeC -0.8 -1.1 Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq) compare
b2375 yfdX -0.8 -1.4 hypothetical protein (NCBI) compare
b4179 vacB -0.8 -2.5 putative enzyme (VIMSS) compare
b2314 dedD -0.7 -2.1 putative lipoprotein (VIMSS) compare
b1600 mdtJ -0.7 -0.8 multidrug efflux system transporter (NCBI) compare
b3728 pstS -0.7 -1.0 phosphate transporter subunit (NCBI) compare
b1773 ydjI -0.7 -0.8 predicted aldolase (NCBI) compare
b1707 ydiV -0.7 -1.6 hypothetical protein (NCBI) compare
b3169 nusA -0.7 -1.7 transcription elongation factor NusA (NCBI) compare
b0122 yacC -0.7 -2.1 orf, hypothetical protein (VIMSS) compare
b1503 ydeR -0.7 -1.5 predicted fimbrial-like adhesin protein (NCBI) compare
b3446 yrhB -0.7 -2.4 hypothetical protein (NCBI) compare
b2111 yehD -0.7 -1.4 predicted fimbrial-like adhesin protein (NCBI) compare
b0013 yaaI -0.7 -2.8 hypothetical protein (NCBI) compare
b4151 frdD -0.7 -1.9 fumarate reductase subunit D (NCBI) compare
b3500 gor -0.7 -1.0 glutathione reductase (NCBI) compare
b1594 dgsA -0.7 -1.9 DNA-binding transcriptional repressor (NCBI) compare
b1130 phoP -0.7 -0.5 DNA-binding response regulator in two-component regulatory system with PhoQ (NCBI) compare
b1648 b1648 -0.7 -1.2 orf, hypothetical protein (VIMSS) compare
b3620 rfaF -0.7 -2.5 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b3545 proK -0.7 -1.1 tRNA-Pro (NCBI) compare
b2037 rfbX -0.7 -3.3 predicted polisoprenol-linked O-antigen transporter (NCBI) compare
b1690 ydiM -0.7 -0.8 predicted transporter (NCBI) compare
b3794 rffM -0.7 -3.5 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b2809 b2809 -0.7 -1.6 orf, hypothetical protein (VIMSS) compare
b2504 yfgG -0.7 -0.4 Negative regulator of nickel and cobalt uptake (YfgG) (from data) compare
b1877 yecT -0.7 -1.0 orf, hypothetical protein (VIMSS) compare
b2330 yfcB -0.7 -0.7 putative adenine-specific methylase (VIMSS) compare
b1129 phoQ -0.7 -0.4 sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI) compare
b0535 fimZ -0.7 -1.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b2531 iscR -0.7 -0.8 DNA-binding transcriptional repressor (NCBI) compare
b0740 tolB -0.7 -1.6 translocation protein TolB precursor (NCBI) compare
b0890 ftsK -0.7 -1.2 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b4313 fimE -0.7 -3.3 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b1634 tppB -0.7 -2.2 putative tripeptide transporter permease (NCBI) compare
b2189 proL -0.7 -1.1 tRNA-Pro (NCBI) compare
b1524 yneH -0.7 -0.7 predicted glutaminase (NCBI) compare
b1040 csgD -0.7 -0.7 DNA-binding transcriptional activator in two-component regulatory system (NCBI) compare
b1042 csgA -0.7 -3.6 cryptic curlin major subunit (NCBI) compare
b0481 ybaK -0.7 -2.3 hypothetical protein (NCBI) compare
b1168 b1168 -0.7 -1.5 putative proteases (VIMSS) compare
b0297 eaeH -0.7 -2.4 attaching and effacing protein, pathogenesis factor (RefSeq) compare
b1255 yciC -0.7 -2.3 hypothetical protein (NCBI) compare
b1182 hlyE -0.7 -0.4 hemolysin E (VIMSS) compare
b1165 ymgA -0.7 -0.4 hypothetical protein (NCBI) compare
b0706 ybfD -0.7 -0.9 hypothetical protein (NCBI) compare
b4497 yeeL -0.7 -0.4 no description compare
b0630 lipB -0.6 -0.4 protein of lipoate biosynthesis (VIMSS) compare
b1084 rne -0.6 -2.2 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b3134 agaW -0.6 -1.9 N-acetylgalactosamine-specific enzyme IIC component of PTS, fragment (pseudogene) (RefSeq) compare
b4045 yjbJ -0.6 -1.4 predicted stress response protein (NCBI) compare
b4561 insM -0.6 -2.2 transposase (RefSeq) compare


Specific Phenotypes

For 4 genes in this experiment

For stress Hygromycin B in Escherichia coli BW25113

For stress Hygromycin B across organisms