Experiment set8IT086 for Escherichia coli BW25113

Compare to:

LB Aerobic with Trimethoprim 0.00000125 mM

200 most important genes:

  gene name fitness t score description  
b0920 ycbC -5.4 -3.7 conserved inner membrane protein (NCBI) compare
b2175 spr -3.5 -4.1 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b0462 acrB -3.4 -16.5 multidrug efflux system protein (NCBI) compare
b0149 mrcB -3.3 -13.2 penicillin-binding protein 1b (NCBI) compare
b1865 nudB -2.8 -1.9 dATP pyrophosphohydrolase (NCBI) compare
b0463 acrA -2.8 -11.5 multidrug efflux system (NCBI) compare
b4699 fnrS -2.5 -1.4 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b1830 prc -2.5 -3.7 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b0957 ompA -2.5 -3.6 outer membrane protein A (3a;II*;G;d) (NCBI) compare
b3205 yhbJ -2.4 -3.6 hypothetical protein (NCBI) compare
b0683 fur -2.3 -1.5 ferric uptake regulator (NCBI) compare
b0088 murD -2.2 -2.8 UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI) compare
b3035 tolC -2.1 -7.9 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b1855 lpxM -2.1 -5.1 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b1863 ruvC -2.1 -1.4 Holliday junction resolvase (NCBI) compare
b0142 folK -2.1 -1.1 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b1882 cheY -2.1 -1.1 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b1279 yciS -2.0 -1.5 conserved inner membrane protein (NCBI) compare
b1861 ruvA -2.0 -1.3 Holliday junction DNA helicase motor protein (NCBI) compare
b0726 sucA -2.0 -3.5 alpha-ketoglutarate decarboxylase (NCBI) compare
b1105 ycfM -2.0 -4.8 predicted outer membrane lipoprotein (NCBI) compare
b0617 citD -1.9 -2.8 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b1506 yneL -1.9 -1.3 predicted transcriptional regulator (NCBI) compare
b4701 sokX -1.8 -1.2 sok-related sRNA, function unknown (RefSeq) compare
b0018 mokC -1.7 -1.9 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b2501 ppk -1.7 -7.6 polyphosphate kinase (NCBI) compare
b2363 yfdT -1.7 -1.9 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b3792 wzxE -1.7 -5.0 O-antigen translocase (NCBI) compare
b1860 ruvB -1.7 -1.1 Holliday junction DNA helicase B (NCBI) compare
b3977 tyrU -1.7 -1.3 tRNA-Tyr (NCBI) compare
b3784 rfe -1.6 -10.7 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b4594 ymgJ -1.6 -1.7 hypothetical protein (RefSeq) compare
b0053 surA -1.5 -3.6 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b1192 ldcA -1.5 -4.5 L,D-carboxypeptidase A (NCBI) compare
b3630 rfaP -1.5 -6.6 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b0738 tolR -1.5 -2.5 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b1084 rne -1.5 -4.6 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b3387 dam -1.5 -8.4 DNA adenine methylase (NCBI) compare
b3082 ygjM -1.5 -1.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b2037 rfbX -1.5 -5.5 predicted polisoprenol-linked O-antigen transporter (NCBI) compare
b4481 rffT -1.4 -5.7 4-alpha-L-fucosyltransferase (NCBI) compare
b2518 ndk -1.4 -2.0 nucleoside diphosphate kinase (NCBI) compare
b3620 rfaF -1.4 -4.2 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b3641 ttk -1.4 -7.1 putative transcriptional regulator (VIMSS) compare
b1235 rssB -1.4 -5.7 response regulator of RpoS (NCBI) compare
b3791 rffA -1.4 -6.2 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b3163 nlpI -1.4 -3.0 hypothetical protein (NCBI) compare
b3026 qseC -1.4 -2.0 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b2279 nuoK -1.3 -2.0 NADH dehydrogenase subunit K (NCBI) compare
b4676 yoaK -1.3 -0.8 expressed protein (RefSeq) compare
b3842 rfaH -1.3 -4.2 transcriptional activator RfaH (NCBI) compare
b0740 tolB -1.3 -2.8 translocation protein TolB precursor (NCBI) compare
b1936 intG -1.3 -0.8 predicted defective phage integrase (pseudogene) (RefSeq) compare
b2299 yfcD -1.3 -4.0 predicted NUDIX hydrolase (NCBI) compare
b4201 priB -1.3 -2.2 primosomal replication protein N (NCBI) compare
b3621 rfaC -1.3 -3.3 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b1198 dhaM -1.3 -4.5 fused predicted dihydroxyacetone-specific PTS enzymes: HPr component/EI component (RefSeq) compare
b1429 tehA -1.3 -1.0 potassium-tellurite ethidium and proflavin transporter (NCBI) compare
b3790 rffC -1.2 -4.3 TDP-fucosamine acetyltransferase (RefSeq) compare
b0437 clpP -1.2 -4.0 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b1075 flgD -1.2 -2.8 flagellar basal body rod modification protein D (NCBI) compare
b1286 rnb -1.2 -1.7 exoribonuclease II (NCBI) compare
b1524 yneH -1.2 -1.1 predicted glutaminase (NCBI) compare
b3627 rfaI -1.2 -5.3 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b1556 b1556 -1.2 -1.5 orf, hypothetical protein (VIMSS) compare
b2017 yefM -1.2 -1.7 orf, hypothetical protein (VIMSS) compare
b4520 ymgF -1.2 -1.0 hypothetical protein (NCBI) compare
b1531 marA -1.2 -3.2 multiple antibiotic resistance; transcriptional activator of defense systems (VIMSS) compare
b0840 deoR -1.2 -3.5 DNA-binding transcriptional repressor (NCBI) compare
b1739 osmE -1.2 -2.2 DNA-binding transcriptional activator (NCBI) compare
b3962 udhA -1.2 -4.2 putative oxidoreductase (VIMSS) compare
b3443 yrhA -1.2 -0.8 orf, hypothetical protein (VIMSS) compare
b3652 recG -1.1 -5.8 ATP-dependent DNA helicase (NCBI) compare
b1620 malI -1.1 -2.0 DNA-binding transcriptional repressor (NCBI) compare
b4413 sokC -1.1 -1.8 regulatory antisense RNA (NCBI) compare
b3199 yrbK -1.1 -2.3 hypothetical protein (NCBI) compare
b1677 lpp -1.1 -1.5 murein lipoprotein (NCBI) compare
b3631 rfaG -1.1 -7.4 glucosyltransferase I (NCBI) compare
b3229 sspA -1.1 -1.8 stringent starvation protein A (NCBI) compare
b0605 ahpC -1.1 -2.9 alkyl hydroperoxide reductase, C22 subunit (NCBI) compare
b1667 ydhR -1.1 -2.2 hypothetical protein (NCBI) compare
b0989 cspH -1.1 -1.5 stress protein, member of the CspA-family (NCBI) compare
b1346 ydaQ -1.1 -1.1 orf, hypothetical protein (VIMSS) compare
b2377 yfdY -1.1 -2.6 predicted inner membrane protein (NCBI) compare
b1238 tdk -1.1 -2.7 thymidine kinase (NCBI) compare
b2168 fruK -1.0 -1.4 1-phosphofructokinase (NCBI) compare
b4596 yciZ -1.0 -1.5 hypothetical protein (RefSeq) compare
b3742 mioC -1.0 -3.7 flavodoxin (NCBI) compare
b2783 chpR -1.0 -2.0 antitoxin of the ChpA-ChpR toxin-antitoxin system (NCBI) compare
b4515 ybgT -1.0 -1.7 hypothetical protein (NCBI) compare
b4698 mgrR -1.0 -2.0 sRNA antisense regulator down regulates YgdQ and EptB, PhoPQ-regulated, Hfq-dependent; affects sensitivity to antimicrobial peptides (RefSeq) compare
b2376 ypdI -1.0 -2.2 predicted lipoprotein involved in colanic acid biosynthesis (NCBI) compare
b0724 sdhB -1.0 -4.3 succinate dehydrogenase, FeS subunit (NCBI) compare
b3813 uvrD -1.0 -7.3 DNA-dependent ATPase I and helicase II (NCBI) compare
b1348 lar -1.0 -1.3 Rac prophage; restriction alleviation protein (NCBI) compare
b4202 rpsR -1.0 -1.0 30S ribosomal protein S18 (NCBI) compare
b2494 yfgC -1.0 -4.9 predicted peptidase (NCBI) compare
b0723 sdhA -1.0 -6.1 succinate dehydrogenase flavoprotein subunit (NCBI) compare
b4595 yciY -1.0 -2.0 hypothetical protein (RefSeq) compare
b0178 hlpA -1.0 -2.9 periplasmic chaperone (NCBI) compare
b3052 rfaE -1.0 -3.2 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b4246 pyrL -0.9 -2.1 pyrBI operon leader peptide (NCBI) compare
b2114 metG -0.9 -1.3 methionyl-tRNA synthetase (NCBI) compare
b0737 tolQ -0.9 -1.3 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b2512 yfgL -0.9 -3.3 protein assembly complex, lipoprotein component (NCBI) compare
b3613 yibP -0.9 -1.6 putative membrane protein (VIMSS) compare
b2038 rfbC -0.9 -4.0 dTDP-4-deoxyrhamnose-3,5-epimerase (NCBI) compare
b1187 fadR -0.9 -2.8 fatty acid metabolism regulator (NCBI) compare
b3237 argR -0.9 -2.6 arginine repressor (NCBI) compare
b4598 yncL -0.9 -1.9 hypothetical protein (RefSeq) compare
b3794 rffM -0.9 -4.6 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b3169 nusA -0.9 -1.9 transcription elongation factor NusA (NCBI) compare
b1594 dgsA -0.9 -2.2 DNA-binding transcriptional repressor (NCBI) compare
b1259 yciG -0.9 -1.1 orf, hypothetical protein (VIMSS) compare
b3619 rfaD -0.9 -2.1 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b1731 cedA -0.9 -2.1 cell division modulator (RefSeq) compare
b1382 ynbE -0.9 -1.3 predicted lipoprotein (NCBI) compare
b3806 cyaA -0.9 -3.0 adenylate cyclase (NCBI) compare
b1919 yedO -0.9 -2.6 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b1642 slyA -0.9 -1.1 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b0092 ddlB -0.8 -3.3 D-alanylalanine synthetase (NCBI) compare
b0082 mraW -0.8 -1.9 S-adenosyl-methyltransferase (NCBI) compare
b2617 smpA -0.8 -1.8 small membrane protein A (VIMSS) compare
b1573 ydfC -0.8 -1.1 Qin prophage; predicted protein (RefSeq) compare
b0632 dacA -0.8 -4.3 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b1877 yecT -0.8 -0.8 orf, hypothetical protein (VIMSS) compare
b0641 rlpB -0.8 -1.7 minor lipoprotein (NCBI) compare
b1984 asnW -0.8 -1.0 tRNA-Asn (NCBI) compare
b4655 ythA -0.8 -1.0 expressed protein (RefSeq) compare
b2445 yffN -0.8 -2.2 CPZ-55 prophage; predicted protein (RefSeq) compare
b2288 nuoA -0.8 -1.8 NADH dehydrogenase I chain A (VIMSS) compare
b3911 cpxA -0.8 -5.2 two-component sensor protein (NCBI) compare
b0864 artP -0.8 -2.2 arginine transporter subunit (NCBI) compare
b1195 ymgE -0.8 -2.1 predicted inner membrane protein (NCBI) compare
b1540 ydfH -0.8 -1.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b4242 mgtA -0.8 -4.5 magnesium transporter (NCBI) compare
b1762 b1762 -0.8 -1.0 orf, hypothetical protein (VIMSS) compare
b3628 rfaB -0.8 -4.0 UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase (RefSeq) compare
b1514 lsrC -0.8 -1.1 AI2 transporter (NCBI) compare
b3629 rfaS -0.8 -1.5 lipopolysaccharide core biosynthesis protein (NCBI) compare
b2808 gcvA -0.8 -2.2 DNA-binding transcriptional dual regulator (NCBI) compare
b1605 ydgI -0.8 -2.7 predicted arginine/ornithine antiporter transporter (NCBI) compare
b2189 proL -0.8 -1.2 tRNA-Pro (NCBI) compare
b0722 sdhD -0.7 -1.9 succinate dehydrogenase cytochrome b556 small membrane subunit (NCBI) compare
b2110 yehC -0.7 -0.5 predicted periplasmic pilin chaperone (NCBI) compare
b2277 nuoM -0.7 -2.8 NADH dehydrogenase subunit M (NCBI) compare
b4557 yidD -0.7 -2.1 hypothetical protein (NCBI) compare
b3609 secB -0.7 -2.2 export protein SecB (NCBI) compare
b2893 dsbC -0.7 -3.4 protein disulfide isomerase II (NCBI) compare
b1079 flgH -0.7 -2.1 flagellar L-ring protein precursor H (NCBI) compare
b4570 lomR -0.7 -1.0 no description compare
b3728 pstS -0.7 -1.1 phosphate transporter subunit (NCBI) compare
b0627 tatE -0.7 -1.1 twin arginine translocase protein E (NCBI) compare
b2276 nuoN -0.7 -2.5 NADH:ubiquinone oxidoreductase, membrane subunit N (RefSeq) compare
b3131 agaR -0.7 -2.3 DNA-binding transcriptional dual regulator (NCBI) compare
b3870 glnA -0.7 -1.3 glutamine synthetase (NCBI) compare
b1575 dicB -0.7 -1.0 Qin prophage; cell division inhibition protein (NCBI) compare
b0706 ybfD -0.7 -0.9 hypothetical protein (NCBI) compare
b1954 dsrA -0.7 -1.4 regulatory antisense RNA (NCBI) compare
b1103 ycfF -0.7 -0.9 orf, hypothetical protein (VIMSS) compare
b1278 pgpB -0.7 -1.3 phosphatidylglycerophosphatase B (NCBI) compare
b2393 nupC -0.7 -3.2 nucleoside (except guanosine) transporter (NCBI) compare
b2504 yfgG -0.7 -0.4 Negative regulator of nickel and cobalt uptake (YfgG) (from data) compare
b3164 pnp -0.7 -1.2 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b0460 hha -0.7 -1.7 modulator of gene expression, with H-NS (NCBI) compare
b1219 ychN -0.7 -2.0 hypothetical protein (NCBI) compare
b3203 yhbH -0.7 -2.3 predicted ribosome-associated, sigma 54 modulation protein (NCBI) compare
b3726 pstA -0.7 -2.2 phosphate transporter subunit (NCBI) compare
b1951 rcsA -0.7 -0.4 DNA-binding transcriptional activator, co-regulator with RcsB (NCBI) compare
b2498 upp -0.7 -1.2 uracil phosphoribosyltransferase (VIMSS) compare
b1583 ynfB -0.6 -1.2 hypothetical protein (NCBI) compare
b3101 yqjF -0.6 -1.4 orf, hypothetical protein (VIMSS) compare
b4171 miaA -0.6 -1.2 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b4640 yoeG -0.6 -0.9 no description compare
b2278 nuoL -0.6 -3.7 NADH dehydrogenase subunit L (NCBI) compare
b1607 ydgC -0.6 -1.0 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b3787 rffD -0.6 -4.1 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b1523 yneG -0.6 -1.4 hypothetical protein (NCBI) compare
b4493 gapC -0.6 -1.3 no description compare
b0125 hpt -0.6 -2.6 hypoxanthine phosphoribosyltransferase (VIMSS) compare
b2807 ygdD -0.6 -2.3 conserved inner membrane protein (NCBI) compare
b1330 ynaI -0.6 -1.7 conserved inner membrane protein (NCBI) compare
b2945 endA -0.6 -3.0 DNA-specific endonuclease I (NCBI) compare
b4025 pgi -0.6 -3.3 glucose-6-phosphate isomerase (NCBI) compare
b1335 ogt -0.6 -1.4 O-6-alkylguanine-DNA:cysteine-protein methyltransferase (NCBI) compare
b2215 ompC -0.6 -3.5 outer membrane porin protein C (NCBI) compare
b0222 lpcA -0.6 -1.2 phosphoheptose isomerase (NCBI) compare
b2287 nuoB -0.6 -1.6 NADH dehydrogenase subunit B (NCBI) compare
b2683 ygaH -0.6 -3.5 predicted inner membrane protein (NCBI) compare
b4503 yafF -0.6 -0.5 no description compare
b0857 potI -0.6 -1.9 putrescine transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1161 ycgX -0.6 -0.4 hypothetical protein (NCBI) compare
b2623 yfjH -0.6 -2.0 CP4-57 prophage; predicted protein (NCBI) compare
b2579 yfiD -0.6 -1.1 pyruvate formate lyase subunit (NCBI) compare
b0688 pgm -0.6 -1.3 phosphoglucomutase (NCBI) compare
b0890 ftsK -0.6 -0.9 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b2350 yfdG -0.6 -3.0 CPS-53 (KpLE1) prophage; bactoprenol-linked glucose translocase (flippase) (NCBI) compare
b3979 thrT -0.6 -0.3 tRNA-Thr (NCBI) compare
b1253 yciA -0.6 -1.1 predicted hydrolase (NCBI) compare
b4410 ecnA -0.6 -1.0 entericidin A membrane lipoprotein, antidote entericidin B (NCBI) compare


Specific Phenotypes

For 3 genes in this experiment

For stress Trimethoprim in Escherichia coli BW25113

For stress Trimethoprim across organisms