Experiment set8IT085 for Pseudomonas putida KT2440

40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Group: reactor
Media: M9_1percentGlucose, pH=6.9
Culturing: Putida_ML5_JBEI, 2L Sartorius Biostat reactor, Aerobic, at 30 (C)
By: Thomas Eng on 5/20/18
Media components: 10 g/L D-Glucose, 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride

Specific Phenotypes

For 4 genes in this experiment

SEED Subsystems

Subsystem	#Specific
Orphan regulatory proteins	1
Universal stress protein family	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.