Experiment set8IT078 for Escherichia coli BW25113

LB Aerobic with Novobiocin sodium salt 0.0000625 mM

Group: stress
Media: LB + Novobiocin sodium salt (6.25E-02 mM)
Culturing: Keio_ML9a, 96 deep-well microplate; Multitron, Aerobic, at 37 (C), shaken=750 rpm
By: Hans_Hualan on 7/20/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 16 genes in this experiment

For stress Novobiocin sodium salt in Escherichia coli BW25113

For stress Novobiocin sodium salt across organisms

SEED Subsystems

Subsystem	#Specific
Cysteine Biosynthesis	2
Ton and Tol transport systems	1
Transcription initiation, bacterial sigma factors	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Ubiquinone and menaquinone biosynthesis
• Cysteine metabolism
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Selenoamino acid metabolism
• Naphthalene and anthracene degradation
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Sulfur metabolism
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
di-trans,poly-cis-undecaprenyl phosphate biosynthesis	2	2	1
L-cysteine biosynthesis I	2	2	1
L-cysteine biosynthesis VII (from S-sulfo-L-cysteine)	4	3	1
polyisoprenoid biosynthesis (E. coli)	5	5	1
seleno-amino acid biosynthesis (plants)	5	3	1
hydrogen sulfide biosynthesis II (mammalian)	6	1	1
superpathway of sulfate assimilation and cysteine biosynthesis	9	9	1
superpathway of seleno-compound metabolism	19	6	1