Experiment set8IT078 for Escherichia coli BW25113

LB Aerobic with Novobiocin sodium salt 0.0000625 mM

Group: stress
Media: LB + Novobiocin sodium salt (6.25E-02 mM)
Culturing: Keio_ML9a, 96 deep-well microplate; Multitron, Aerobic, at 37 (C), shaken=750 rpm
By: Hans_Hualan on 7/20/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 19 genes in this experiment

For stress Novobiocin sodium salt in Escherichia coli BW25113

For stress Novobiocin sodium salt across organisms

SEED Subsystems

Subsystem	#Specific
Cysteine Biosynthesis	2
Acid resistance mechanisms	1
Arginine and Ornithine Degradation	1
Polyamine Metabolism	1
Ton and Tol transport systems	1
Transcription initiation, bacterial sigma factors	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Ubiquinone and menaquinone biosynthesis
• Urea cycle and metabolism of amino groups
• Puromycin biosynthesis
• Cysteine metabolism
• Arginine and proline metabolism
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Selenoamino acid metabolism
• Nucleotide sugars metabolism
• alpha-Linolenic acid metabolism
• Naphthalene and anthracene degradation
• Trinitrotoluene degradation
• Sulfur metabolism
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
arginine dependent acid resistance	1	1	1
di-trans,poly-cis-undecaprenyl phosphate biosynthesis	2	2	1
putrescine biosynthesis I	2	2	1
L-arginine degradation III (arginine decarboxylase/agmatinase pathway)	2	2	1
L-cysteine biosynthesis I	2	2	1
L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway)	3	1	1
putrescine biosynthesis II	3	1	1
L-cysteine biosynthesis VII (from S-sulfo-L-cysteine)	4	3	1
superpathway of putrescine biosynthesis	4	3	1
spermidine biosynthesis III	4	2	1
polyisoprenoid biosynthesis (E. coli)	5	5	1
seleno-amino acid biosynthesis (plants)	5	3	1
hydrogen sulfide biosynthesis II (mammalian)	6	1	1
superpathway of polyamine biosynthesis I	8	7	1
superpathway of polyamine biosynthesis II	8	4	1
superpathway of sulfate assimilation and cysteine biosynthesis	9	9	1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	11	11	1
superpathway of L-arginine and L-ornithine degradation	13	13	1
superpathway of arginine and polyamine biosynthesis	17	16	1
superpathway of seleno-compound metabolism	19	6	1