Experiment set8IT060 for Escherichia coli BW25113

LB Aerobic with Chlorhexidine diacetate salt hydrate 0.000000625 mM

Group: stress
Media: LB + Chlorhexidine diacetate salt hydrate (6.25E-04 mM)
Culturing: Keio_ML9a, 96 deep-well microplate; Multitron, Aerobic, at 37 (C), shaken=750 rpm
By: Hans_Hualan on 7/20/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 41 genes in this experiment

For stress Chlorhexidine diacetate salt hydrate in Escherichia coli BW25113

For stress Chlorhexidine diacetate salt hydrate across organisms

SEED Subsystems

Subsystem	#Specific
Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes	2
Proteasome bacterial	2
Proteolysis in bacteria, ATP-dependent	2
Capsular heptose biosynthesis	1
Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate)	1
Folate Biosynthesis	1
Formate dehydrogenase	1
Formate hydrogenase	1
Fructose utilization	1
Glycine and Serine Utilization	1
LOS core oligosaccharide biosynthesis	1
Oxidative stress	1
Pentose phosphate pathway	1
Purine conversions	1
Pyruvate Alanine Serine Interconversions	1
Selenocysteine metabolism	1
Thioredoxin-disulfide reductase	1
Threonine anaerobic catabolism gene cluster	1
Universal stress protein family	1
ZZ gjo need homes	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Porphyrin and chlorophyll metabolism
• Biosynthesis of alkaloids derived from shikimate pathway
• Pentose phosphate pathway
• Pentose and glucuronate interconversions
• Galactose metabolism
• Ubiquinone and menaquinone biosynthesis
• Purine metabolism
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Selenoamino acid metabolism
• Glycerolipid metabolism
• Inositol phosphate metabolism
• Naphthalene and anthracene degradation
• Glyoxylate and dicarboxylate metabolism
• Methane metabolism
• Carotenoid biosynthesis - General
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Drug metabolism - other enzymes
• Biosynthesis of ansamycins
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
formate oxidation to CO2	1	1	1
siroheme biosynthesis	4	4	2
guanine and guanosine salvage II	2	2	1
formate to trimethylamine N-oxide electron transfer	2	2	1
xanthine and xanthosine salvage	2	2	1
di-trans,poly-cis-undecaprenyl phosphate biosynthesis	2	2	1
guanine and guanosine salvage I	2	2	1
formate to dimethyl sulfoxide electron transfer	2	2	1
nitrate reduction III (dissimilatory)	2	2	1
superpathway of guanine and guanosine salvage	3	3	1
adenine salvage	3	3	1
formate to nitrite electron transfer	3	2	1
factor 430 biosynthesis	7	3	2
adenine and adenosine salvage III	4	4	1
oxalate degradation VI	4	2	1
GDP-D-glycero-α-D-manno-heptose biosynthesis	4	1	1
pentose phosphate pathway (non-oxidative branch) I	5	5	1
ADP-L-glycero-β-D-manno-heptose biosynthesis	5	5	1
polyisoprenoid biosynthesis (E. coli)	5	5	1
oxalate degradation III	5	2	1
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation)	13	2	2
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion)	15	3	2
pentose phosphate pathway	8	8	1
formaldehyde assimilation II (assimilatory RuMP Cycle)	9	7	1
Rubisco shunt	10	9	1
formaldehyde assimilation III (dihydroxyacetone cycle)	12	10	1
superpathway of C1 compounds oxidation to CO2	12	4	1
superpathway of purine nucleotide salvage	14	13	1
Bifidobacterium shunt	15	13	1
purine nucleobases degradation I (anaerobic)	15	6	1
adenosylcobalamin biosynthesis II (aerobic)	33	17	2
superpathway of glucose and xylose degradation	17	17	1
adenosylcobalamin biosynthesis I (anaerobic)	36	16	2
purine nucleobases degradation II (anaerobic)	24	17	1