Experiment set8IT058 for Escherichia coli BW25113

LB Aerobic with 2-Mercaptopyridine N-oxide sodium salt 0.0000078125 mM

Group: stress
Media: LB + 2-Mercaptopyridine N-oxide sodium salt (7.81E-03 mM)
Culturing: Keio_ML9a, 96 deep-well microplate; Multitron, Aerobic, at 37 (C), shaken=750 rpm
By: Hans_Hualan on 7/20/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 13 genes in this experiment

For stress 2-Mercaptopyridine N-oxide sodium salt in Escherichia coli BW25113

For stress 2-Mercaptopyridine N-oxide sodium salt across organisms

SEED Subsystems

Subsystem	#Specific
Oxidative stress	2
CBSS-562.2.peg.5158 SK3 including	1
DNA repair, bacterial	1
Nitric oxide synthase	1
Peptidyl-prolyl cis-trans isomerase	1
Potassium homeostasis	1
Transport of Manganese	1
Transport of Zinc	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Porphyrin and chlorophyll metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
siroheme biosynthesis	4	4	2
superoxide radicals degradation	2	2	1
factor 430 biosynthesis	7	3	2
reactive oxygen species degradation	4	4	1
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation)	13	2	2
ethene biosynthesis III (microbes)	7	6	1
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion)	15	3	2
NiFe(CO)(CN)2 cofactor biosynthesis	10	9	1
adenosylcobalamin biosynthesis II (aerobic)	33	17	2
adenosylcobalamin biosynthesis I (anaerobic)	36	16	2