Experiment set8IT054 for Escherichia coli BW25113

LB Aerobic with Irgasan 0.0000003125 mM

Group: stress
Media: LB + Irgasan (3.13E-04 mM)
Culturing: Keio_ML9a, 96 deep-well microplate; Multitron, Aerobic, at 37 (C), shaken=750 rpm
By: Hans_Hualan on 7/20/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 37 genes in this experiment

For stress Irgasan in Escherichia coli BW25113

For stress Irgasan across organisms

SEED Subsystems

Subsystem	#Specific
Coenzyme A Biosynthesis	3
Chitin and N-acetylglucosamine utilization	2
DNA repair, bacterial	2
Orphan regulatory proteins	2
Sialic Acid Metabolism	2
Type IV pilus	2
Alkylphosphonate utilization	1
Biotin biosynthesis	1
CBSS-562.2.peg.5158 SK3 including	1
Capsular heptose biosynthesis	1
Glycine and Serine Utilization	1
LOS core oligosaccharide biosynthesis	1
Lipoic acid metabolism	1
Listeria phi-A118-like prophages	1
Peptidoglycan Biosynthesis	1
Proteasome bacterial	1
Proteolysis in bacteria, ATP-dependent	1
Pyruvate Alanine Serine Interconversions	1
Selenocysteine metabolism	1
Threonine anaerobic catabolism gene cluster	1
UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis	1
Universal stress protein family	1
n-Phenylalkanoic acid degradation	1
tRNA processing	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Galactose metabolism
• Fatty acid metabolism
• Purine metabolism
• Nucleotide sugars metabolism
• Aminosugars metabolism
• Glycerophospholipid metabolism
• Pantothenate and CoA biosynthesis
• Glycolysis / Gluconeogenesis
• Pentose and glucuronate interconversions
• Fructose and mannose metabolism
• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Pyrimidine metabolism
• Alanine and aspartate metabolism
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• beta-Alanine metabolism
• Selenoamino acid metabolism
• Starch and sucrose metabolism
• Inositol phosphate metabolism
• alpha-Linolenic acid metabolism
• Naphthalene and anthracene degradation
• Lipoic acid metabolism
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Aminoacyl-tRNA biosynthesis
• Insect hormone biosynthesis
• Biosynthesis of ansamycins
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
long-chain fatty acid activation	1	1	1
cis-cyclopropane fatty acid (CFA) biosynthesis	1	1	1
β-alanine biosynthesis III	1	1	1
pseudouridine degradation	2	2	1
polyphosphate metabolism	2	2	1
N-acetylglucosamine degradation I	2	2	1
phosphatidylcholine acyl editing	4	2	2
γ-linolenate biosynthesis II (animals)	2	1	1
linoleate biosynthesis II (animals)	2	1	1
sterculate biosynthesis	2	1	1
lipoate salvage I	2	1	1
cinnamoyl-CoA biosynthesis	2	1	1
N-acetylglucosamine degradation II	3	3	1
L-selenocysteine biosynthesis I (bacteria)	3	3	1
3-methyl-branched fatty acid α-oxidation	6	3	2
heptadecane biosynthesis	3	1	1
alkane biosynthesis II	3	1	1
oleate biosynthesis I (plants)	3	1	1
alkane biosynthesis I	3	1	1
phosphopantothenate biosynthesis I	4	4	1
phytol degradation	4	3	1
phosphopantothenate biosynthesis III (archaea)	4	2	1
GDP-D-glycero-α-D-manno-heptose biosynthesis	4	1	1
wax esters biosynthesis II	4	1	1
lipoate salvage II	4	1	1
pinosylvin metabolism	4	1	1
long chain fatty acid ester synthesis (engineered)	4	1	1
superpathway of coenzyme A biosynthesis I (bacteria)	9	9	2
sporopollenin precursors biosynthesis	18	5	4
ADP-L-glycero-β-D-manno-heptose biosynthesis	5	5	1
5,6-dehydrokavain biosynthesis (engineered)	10	8	2
sphingosine and sphingosine-1-phosphate metabolism	10	4	2
octane oxidation	5	2	1
benzoate biosynthesis III (CoA-dependent, non-β-oxidative)	5	2	1
phospholipases	5	1	1
ppGpp metabolism	6	6	1
superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation	6	6	1
stearate biosynthesis II (bacteria and plants)	6	5	1
fatty acid salvage	6	5	1
stearate biosynthesis IV	6	4	1
6-gingerol analog biosynthesis (engineered)	6	2	1
stearate biosynthesis I (animals)	6	1	1
UDP-N-acetyl-D-galactosamine biosynthesis II	7	5	1
ceramide degradation by α-oxidation	7	2	1
chitin degradation I (archaea)	7	2	1
icosapentaenoate biosynthesis III (8-desaturase, mammals)	7	1	1
capsaicin biosynthesis	7	1	1
arachidonate biosynthesis III (6-desaturase, mammals)	7	1	1
icosapentaenoate biosynthesis II (6-desaturase, mammals)	7	1	1
chitin derivatives degradation	8	4	1
2-deoxy-D-ribose degradation II	8	3	1
ceramide and sphingolipid recycling and degradation (yeast)	16	4	2
benzoate biosynthesis I (CoA-dependent, β-oxidative)	9	4	1
superpathway of coenzyme A biosynthesis II (plants)	10	5	1
3-phenylpropanoate degradation	10	4	1
suberin monomers biosynthesis	20	3	2
superpathway of fatty acid biosynthesis II (plant)	43	38	4
mycobactin biosynthesis	11	3	1
anandamide biosynthesis I	12	4	1
palmitate biosynthesis II (type II fatty acid synthase)	31	29	2
cutin biosynthesis	16	2	1
superpathway of N-acetylneuraminate degradation	22	22	1
superpathway of fatty acids biosynthesis (E. coli)	53	51	2
palmitate biosynthesis III	29	21	1
oleate β-oxidation	35	32	1
mycolate biosynthesis	205	25	3
superpathway of mycolate biosynthesis	239	26	3