Experiment set8IT038 for Escherichia coli BW25113

Compare to:

LB Aerobic with Trimethoprim 0.00000125 mM

200 most important genes:

  gene name fitness t score description  
b0920 ycbC -5.5 -3.8 conserved inner membrane protein (NCBI) compare
b2175 spr -4.4 -3.0 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b0149 mrcB -3.7 -12.8 penicillin-binding protein 1b (NCBI) compare
b0463 acrA -3.1 -11.9 multidrug efflux system (NCBI) compare
b3205 yhbJ -2.9 -3.8 hypothetical protein (NCBI) compare
b1865 nudB -2.8 -1.9 dATP pyrophosphohydrolase (NCBI) compare
b0462 acrB -2.8 -16.3 multidrug efflux system protein (NCBI) compare
b1105 ycfM -2.5 -5.6 predicted outer membrane lipoprotein (NCBI) compare
b1482 osmC -2.4 -2.7 osmotically inducible, stress-inducible membrane protein (NCBI) compare
b1493 gadB -2.3 -1.3 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b1575 dicB -2.3 -2.1 Qin prophage; cell division inhibition protein (NCBI) compare
b3341 rpsG -2.2 -1.7 30S ribosomal protein S7 (NCBI) compare
b2501 ppk -2.2 -9.0 polyphosphate kinase (NCBI) compare
b1830 prc -2.1 -3.7 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b1103 ycfF -2.1 -2.0 orf, hypothetical protein (VIMSS) compare
b1863 ruvC -2.1 -1.4 Holliday junction resolvase (NCBI) compare
b0142 folK -2.1 -1.1 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b3792 wzxE -2.1 -4.9 O-antigen translocase (NCBI) compare
b0726 sucA -2.0 -3.8 alpha-ketoglutarate decarboxylase (NCBI) compare
b1861 ruvA -2.0 -1.3 Holliday junction DNA helicase motor protein (NCBI) compare
b3035 tolC -1.9 -8.1 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b3199 yrbK -1.9 -3.2 hypothetical protein (NCBI) compare
b3842 rfaH -1.9 -5.2 transcriptional activator RfaH (NCBI) compare
b0683 fur -1.8 -1.6 ferric uptake regulator (NCBI) compare
b3630 rfaP -1.8 -7.1 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b0018 mokC -1.8 -2.0 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b1238 tdk -1.8 -3.4 thymidine kinase (NCBI) compare
b4201 priB -1.7 -2.7 primosomal replication protein N (NCBI) compare
b0053 surA -1.7 -3.4 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b1677 lpp -1.7 -2.4 murein lipoprotein (NCBI) compare
b1324 tpx -1.7 -3.6 thiol peroxidase (NCBI) compare
b3791 rffA -1.7 -7.4 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b0957 ompA -1.7 -3.4 outer membrane protein A (3a;II*;G;d) (NCBI) compare
b1506 yneL -1.7 -1.5 predicted transcriptional regulator (NCBI) compare
b3387 dam -1.6 -9.1 DNA adenine methylase (NCBI) compare
b2038 rfbC -1.6 -6.1 dTDP-4-deoxyrhamnose-3,5-epimerase (NCBI) compare
b0437 clpP -1.5 -4.4 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b2209 eco -1.5 -2.7 ecotin precursor (NCBI) compare
b1192 ldcA -1.5 -4.4 L,D-carboxypeptidase A (NCBI) compare
b0738 tolR -1.5 -2.6 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b1855 lpxM -1.5 -4.1 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b1531 marA -1.5 -3.9 multiple antibiotic resistance; transcriptional activator of defense systems (VIMSS) compare
b3652 recG -1.5 -7.0 ATP-dependent DNA helicase (NCBI) compare
b0740 tolB -1.4 -2.8 translocation protein TolB precursor (NCBI) compare
b0724 sdhB -1.4 -5.3 succinate dehydrogenase, FeS subunit (NCBI) compare
b3962 udhA -1.4 -5.3 putative oxidoreductase (VIMSS) compare
b3641 ttk -1.4 -7.4 putative transcriptional regulator (VIMSS) compare
b4481 rffT -1.4 -5.4 4-alpha-L-fucosyltransferase (NCBI) compare
b3784 rfe -1.4 -9.8 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b3026 qseC -1.4 -2.2 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b4676 yoaK -1.4 -0.8 expressed protein (RefSeq) compare
b3163 nlpI -1.3 -3.7 hypothetical protein (NCBI) compare
b3620 rfaF -1.3 -3.8 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b1084 rne -1.3 -4.1 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b2285 nuoE -1.3 -2.1 NADH dehydrogenase subunit E (NCBI) compare
b1583 ynfB -1.3 -2.1 hypothetical protein (NCBI) compare
b2037 rfbX -1.3 -5.3 predicted polisoprenol-linked O-antigen transporter (NCBI) compare
b2576 srmB -1.3 -2.0 ATP-dependent RNA helicase (NCBI) compare
b4202 rpsR -1.3 -1.1 30S ribosomal protein S18 (NCBI) compare
b1523 yneG -1.3 -2.3 hypothetical protein (NCBI) compare
b2512 yfgL -1.3 -4.4 protein assembly complex, lipoprotein component (NCBI) compare
b3229 sspA -1.3 -1.8 stringent starvation protein A (NCBI) compare
b3631 rfaG -1.3 -8.4 glucosyltransferase I (NCBI) compare
b2743 pcm -1.2 -3.5 protein-L-isoaspartate O-methyltransferase (NCBI) compare
b0436 tig -1.2 -5.9 trigger factor (NCBI) compare
b0088 murD -1.2 -2.0 UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI) compare
b3627 rfaI -1.2 -5.7 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b1187 fadR -1.2 -2.9 fatty acid metabolism regulator (NCBI) compare
b3790 rffC -1.2 -4.0 TDP-fucosamine acetyltransferase (RefSeq) compare
b0438 clpX -1.2 -4.6 ATP-dependent protease ATP-binding subunit (NCBI) compare
b4520 ymgF -1.2 -1.0 hypothetical protein (NCBI) compare
b3052 rfaE -1.1 -3.6 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b4701 sokX -1.1 -1.0 sok-related sRNA, function unknown (RefSeq) compare
b2376 ypdI -1.1 -2.3 predicted lipoprotein involved in colanic acid biosynthesis (NCBI) compare
b4062 soxS -1.1 -3.2 DNA-binding transcriptional dual regulator (NCBI) compare
b3237 argR -1.1 -3.2 arginine repressor (NCBI) compare
b3609 secB -1.1 -3.1 export protein SecB (NCBI) compare
b3742 mioC -1.1 -4.2 flavodoxin (NCBI) compare
b3621 rfaC -1.1 -3.4 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b0178 hlpA -1.0 -3.0 periplasmic chaperone (NCBI) compare
b0632 dacA -1.0 -5.2 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b2506 yfgI -1.0 -1.6 hypothetical protein (NCBI) compare
b2617 smpA -1.0 -2.3 small membrane protein A (VIMSS) compare
b4431 rprA -1.0 -1.6 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b3911 cpxA -1.0 -6.0 two-component sensor protein (NCBI) compare
b2330 yfcB -0.9 -0.8 putative adenine-specific methylase (VIMSS) compare
b0840 deoR -0.9 -3.1 DNA-binding transcriptional repressor (NCBI) compare
b0688 pgm -0.9 -1.9 phosphoglucomutase (NCBI) compare
b0605 ahpC -0.9 -2.7 alkyl hydroperoxide reductase, C22 subunit (NCBI) compare
b1164 ycgZ -0.9 -0.9 hypothetical protein (NCBI) compare
b0641 rlpB -0.9 -1.6 minor lipoprotein (NCBI) compare
b2299 yfcD -0.9 -3.1 predicted NUDIX hydrolase (NCBI) compare
b3628 rfaB -0.9 -4.6 UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase (RefSeq) compare
b3545 proK -0.9 -1.8 tRNA-Pro (NCBI) compare
b2494 yfgC -0.9 -4.6 predicted peptidase (NCBI) compare
b3649 rpoZ -0.9 -1.8 DNA-directed RNA polymerase subunit omega (NCBI) compare
b4242 mgtA -0.9 -5.0 magnesium transporter (NCBI) compare
b1548 nohA -0.9 -1.1 Qin prophage; predicted packaging protein (RefSeq) compare
b2393 nupC -0.9 -4.1 nucleoside (except guanosine) transporter (NCBI) compare
b0723 sdhA -0.9 -5.6 succinate dehydrogenase flavoprotein subunit (NCBI) compare
b2553 glnB -0.9 -2.4 regulatory protein P-II for glutamine synthetase (NCBI) compare
b1882 cheY -0.9 -0.6 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b3813 uvrD -0.9 -6.4 DNA-dependent ATPase I and helicase II (NCBI) compare
b0990 cspG -0.9 -0.5 DNA-binding transcriptional regulator (NCBI) compare
b1604 ydgH -0.8 -2.9 hypothetical protein (NCBI) compare
b1333 uspE -0.8 -1.9 stress-induced protein (NCBI) compare
b2189 proL -0.8 -1.4 tRNA-Pro (NCBI) compare
b0741 pal -0.8 -2.0 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b0318 yahD -0.8 -3.5 predicted transcriptional regulator with ankyrin domain (NCBI) compare
b3787 rffD -0.8 -5.7 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b2744 surE -0.8 -3.9 acid phosphatase (NCBI) compare
b1346 ydaQ -0.8 -0.9 orf, hypothetical protein (VIMSS) compare
b2215 ompC -0.8 -4.4 outer membrane porin protein C (NCBI) compare
b4413 sokC -0.8 -1.4 regulatory antisense RNA (NCBI) compare
b2572 rseA -0.8 -3.3 anti-sigma factor (NCBI) compare
b1591 b1591 -0.8 -1.2 putative oxidoreductase component (VIMSS) compare
b2058 wcaB -0.8 -1.0 predicted acyl transferase (NCBI) compare
b0125 hpt -0.8 -3.2 hypoxanthine phosphoribosyltransferase (VIMSS) compare
b1607 ydgC -0.8 -1.3 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b2968 yghD -0.8 -3.2 predicted secretion pathway M-type protein, membrane anchored (NCBI) compare
b1949 fliQ -0.8 -1.6 flagellar biosynthesis protein Q (NCBI) compare
b3469 zntA -0.8 -3.6 zinc, cobalt and lead efflux system (NCBI) compare
b3619 rfaD -0.8 -1.9 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b0721 sdhC -0.8 -2.3 succinate dehydrogenase cytochrome b556 large membrane subunit (NCBI) compare
b2897 ygfY -0.8 -1.9 hypothetical protein (NCBI) compare
b0028 fkpB -0.8 -2.3 FKBP-type peptidyl-prolyl cis-trans isomerase (rotamase) (NCBI) compare
b0729 sucD -0.8 -2.1 succinyl-CoA synthetase subunit alpha (NCBI) compare
b3404 envZ -0.8 -4.3 osmolarity sensor protein (NCBI) compare
b2518 ndk -0.7 -1.3 nucleoside diphosphate kinase (NCBI) compare
b4699 fnrS -0.7 -0.6 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b3806 cyaA -0.7 -2.8 adenylate cyclase (NCBI) compare
b0092 ddlB -0.7 -3.0 D-alanylalanine synthetase (NCBI) compare
b2808 gcvA -0.7 -2.1 DNA-binding transcriptional dual regulator (NCBI) compare
b0739 tolA -0.7 -1.1 cell envelope integrity inner membrane protein TolA (NCBI) compare
b2990 hybG -0.7 -1.7 hydrogenase 2 accessory protein (NCBI) compare
b4702 mgtL -0.7 -0.9 regulatory leader peptide for mgtA (NCBI) compare
b2003 yeeT -0.7 -2.0 CP4-44 prophage; predicted protein (NCBI) compare
b1259 yciG -0.7 -0.9 orf, hypothetical protein (VIMSS) compare
b1989 asnV -0.7 -1.7 tRNA-Asn (NCBI) compare
b2009 sbmC -0.7 -2.0 DNA gyrase inhibitor (NCBI) compare
b1842 holE -0.7 -0.9 DNA polymerase III, theta subunit (NCBI) compare
b3794 rffM -0.7 -3.9 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b4596 yciZ -0.7 -1.1 hypothetical protein (RefSeq) compare
b4406 yaeP -0.7 -1.6 hypothetical protein (NCBI) compare
b2114 metG -0.7 -1.2 methionyl-tRNA synthetase (NCBI) compare
b1104 ycfL -0.7 -1.1 hypothetical protein (NCBI) compare
b1434 ydcN -0.7 -1.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b0430 cyoC -0.7 -2.3 cytochrome o ubiquinol oxidase subunit III (NCBI) compare
b4025 pgi -0.7 -3.6 glucose-6-phosphate isomerase (NCBI) compare
b4598 yncL -0.7 -1.6 hypothetical protein (RefSeq) compare
b1733 chbG -0.7 -2.6 hypothetical protein (NCBI) compare
b1567 intK -0.7 -1.3 Qin prophage; predicted protein (RefSeq) compare
b3169 nusA -0.7 -1.7 transcription elongation factor NusA (NCBI) compare
b3844 fre -0.7 -2.9 NAD(P)H-flavin reductase (NCBI) compare
b1150 ymfR -0.7 -0.7 e14 prophage; predicted protein (NCBI) compare
b2516 yfgA -0.7 -3.1 hypothetical protein (NCBI) compare
b1235 rssB -0.7 -3.2 response regulator of RpoS (NCBI) compare
b4441 glmY -0.7 -1.3 sRNA activator of glmS mRNA (RefSeq) compare
b0737 tolQ -0.7 -1.0 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b1860 ruvB -0.7 -0.5 Holliday junction DNA helicase B (NCBI) compare
b1689 b1689 -0.7 -1.4 orf, hypothetical protein (VIMSS) compare
b2859 ygeO -0.7 -0.7 hypothetical protein (RefSeq) compare
b2282 nuoH -0.7 -1.6 NADH dehydrogenase subunit H (NCBI) compare
b4313 fimE -0.7 -3.4 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b0782 moaB -0.7 -1.2 molybdopterin biosynthesis protein B (NCBI) compare
b1648 b1648 -0.7 -1.1 orf, hypothetical protein (VIMSS) compare
b3977 tyrU -0.7 -0.7 tRNA-Tyr (NCBI) compare
b1505 ydeT -0.7 -0.6 hypothetical protein (RefSeq) compare
b1236 galU -0.7 -1.6 glucose-1-phosphate uridylyltransferase (NCBI) compare
b1762 b1762 -0.6 -1.2 orf, hypothetical protein (VIMSS) compare
b0222 lpcA -0.6 -1.4 phosphoheptose isomerase (NCBI) compare
b1605 ydgI -0.6 -2.0 predicted arginine/ornithine antiporter transporter (NCBI) compare
b2288 nuoA -0.6 -1.6 NADH dehydrogenase I chain A (VIMSS) compare
b1025 ycdT -0.6 -0.6 predicted diguanylate cyclase (NCBI) compare
b0759 galE -0.6 -2.9 UDP-galactose-4-epimerase (VIMSS) compare
b1536 ydeI -0.6 -0.4 hypothetical protein (NCBI) compare
b2413 cysZ -0.6 -3.1 putative sulfate transport protein CysZ (NCBI) compare
b0877 ybjX -0.6 -3.3 hypothetical protein (NCBI) compare
b1658 purR -0.6 -2.4 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b4246 pyrL -0.6 -1.6 pyrBI operon leader peptide (NCBI) compare
b2894 xerD -0.6 -0.4 site-specific tyrosine recombinase XerD (NCBI) compare
b3786 rffE -0.6 -4.7 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b0862 artQ -0.6 -1.3 arginine transporter subunit (NCBI) compare
b1233 ychJ -0.6 -1.8 hypothetical protein (NCBI) compare
b3195 yrbF -0.6 -4.0 predicted toluene transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b3950 frwB -0.6 -1.6 predicted enzyme IIB component of PTS (NCBI) compare
b2369 evgA -0.6 -1.1 DNA-binding response regulator in two-component regulatory system with EvgS (NCBI) compare
b2784 relA -0.6 -3.9 (p)ppGpp synthetase I/GTP pyrophosphokinase (NCBI) compare
b0082 mraW -0.6 -1.5 S-adenosyl-methyltransferase (NCBI) compare
b4522 yciX_1 -0.6 -1.3 hypothetical protein (NCBI) compare
b2174 b2174 -0.6 -3.3 orf, hypothetical protein (VIMSS) compare
b4552 yrhC -0.6 -0.4 no description compare
b0049 apaH -0.6 -1.1 diadenosinetetraphosphatase (NCBI) compare
b0340 cynS -0.6 -1.7 cyanate hydratase (NCBI) compare
b4519 icdC -0.6 -2.0 no description compare
b2284 nuoF -0.6 -1.9 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b1074 flgC -0.6 -1.1 flagellar basal-body rod protein C (NCBI) compare
b1161 ycgX -0.6 -0.3 hypothetical protein (NCBI) compare
b2317 dedA -0.6 -1.6 conserved inner membrane protein (NCBI) compare
b1466 narW -0.6 -2.2 nitrate reductase 2 (NRZ), delta subunit (assembly subunit) (NCBI) compare


Specific Phenotypes

For 5 genes in this experiment

For stress Trimethoprim in Escherichia coli BW25113

For stress Trimethoprim across organisms