Experiment set8IT038 for Escherichia coli BW25113

LB Aerobic with Trimethoprim 0.00000125 mM

Group: stress
Media: LB + Trimethoprim (1.25E-03 mM)
Culturing: Keio_ML9a, 96 deep-well microplate; Multitron, Aerobic, at 37 (C), shaken=750 rpm
By: Hans_Hualan on 7/20/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 4 genes in this experiment

For stress Trimethoprim in Escherichia coli BW25113

For stress Trimethoprim across organisms

SEED Subsystems

Subsystem	#Specific
Capsular heptose biosynthesis	2
LOS core oligosaccharide biosynthesis	1
Rhamnose containing glycans	1
dTDP-rhamnose synthesis	1
linker unit-arabinogalactan synthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose and glucuronate interconversions
• Galactose metabolism
• Streptomycin biosynthesis
• Polyketide sugar unit biosynthesis
• Inositol phosphate metabolism
• Biosynthesis of ansamycins

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
GDP-D-glycero-α-D-manno-heptose biosynthesis	4	1	1
dTDP-β-L-rhamnose biosynthesis	5	5	1
ADP-L-glycero-β-D-manno-heptose biosynthesis	5	5	1
dTDP-4-O-demethyl-β-L-noviose biosynthesis	5	3	1
dTDP-L-daunosamine biosynthesis	6	3	1
dTDP-sibirosamine biosynthesis	6	3	1
O-antigen building blocks biosynthesis (E. coli)	11	11	1
superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis	19	7	1
superpathway of novobiocin biosynthesis	19	4	1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis	33	14	1