Experiment set8IT033 for Escherichia coli BW25113

Compare to:

LB Aerobic with Doxycycline hyclate 0.00000625 mM

200 most important genes:

  gene name fitness t score description  
b0912 ihfB -4.1 -2.8 integration host factor subunit beta (NCBI) compare
b0462 acrB -3.7 -13.1 multidrug efflux system protein (NCBI) compare
b0463 acrA -3.5 -9.9 multidrug efflux system (NCBI) compare
b4201 priB -3.5 -2.4 primosomal replication protein N (NCBI) compare
b1677 lpp -3.3 -2.3 murein lipoprotein (NCBI) compare
b0605 ahpC -3.2 -3.1 alkyl hydroperoxide reductase, C22 subunit (NCBI) compare
b3035 tolC -3.1 -6.5 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b1531 marA -3.0 -4.4 multiple antibiotic resistance; transcriptional activator of defense systems (VIMSS) compare
b2188 yejM -2.9 -7.6 predicted hydrolase, inner membrane (NCBI) compare
b3842 rfaH -2.8 -3.8 transcriptional activator RfaH (NCBI) compare
b2317 dedA -2.8 -5.1 conserved inner membrane protein (NCBI) compare
b3194 yrbE -2.7 -10.6 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b0178 hlpA -2.7 -4.6 periplasmic chaperone (NCBI) compare
b3341 rpsG -2.6 -1.7 30S ribosomal protein S7 (NCBI) compare
b2174 b2174 -2.6 -7.6 orf, hypothetical protein (VIMSS) compare
b3195 yrbF -2.5 -10.5 predicted toluene transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b3192 yrbC -2.4 -9.1 predicted ABC-type organic solvent transporter (NCBI) compare
b2017 yefM -2.4 -1.4 orf, hypothetical protein (VIMSS) compare
b3465 yhhF -2.4 -6.4 predicted methyltransferase (NCBI) compare
b3191 yrbB -2.4 -9.3 orf, hypothetical protein (VIMSS) compare
b3199 yrbK -2.4 -2.8 hypothetical protein (NCBI) compare
b3632 rfaQ -2.4 -12.9 lipopolysaccharide core biosynthesis protein (NCBI) compare
b0957 ompA -2.4 -3.1 outer membrane protein A (3a;II*;G;d) (NCBI) compare
b3193 yrbD -2.3 -8.8 predicted ABC-type organic solvent transporter (NCBI) compare
b2784 relA -2.3 -9.0 (p)ppGpp synthetase I/GTP pyrophosphokinase (NCBI) compare
b4472 yhdP -2.3 -15.7 conserved membrane protein, predicted transporter (NCBI) compare
b1855 lpxM -2.2 -4.0 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b3163 nlpI -2.2 -3.2 hypothetical protein (NCBI) compare
b4041 plsB -2.1 -2.2 glycerol-3-phosphate acyltransferase (VIMSS) compare
b3784 rfe -2.1 -12.1 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b3052 rfaE -2.1 -3.4 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b3621 rfaC -2.1 -2.0 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b3631 rfaG -2.0 -8.5 glucosyltransferase I (NCBI) compare
b4203 rplI -2.0 -3.7 50S ribosomal protein L9 (NCBI) compare
b4655 ythA -2.0 -1.3 expressed protein (RefSeq) compare
b2346 vacJ -1.9 -6.4 predicted lipoprotein (NCBI) compare
b3021 ygiT -1.9 -1.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b3620 rfaF -1.8 -2.5 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b1830 prc -1.8 -2.7 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b1249 cls -1.8 -5.2 cardiolipin synthetase (NCBI) compare
b4000 hupA -1.8 -3.6 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b1818 manY -1.8 -2.3 mannose-specific enzyme IIC component of PTS (NCBI) compare
b3790 rffC -1.8 -4.4 TDP-fucosamine acetyltransferase (RefSeq) compare
b2168 fruK -1.7 -1.6 1-phosphofructokinase (NCBI) compare
b3761 trpT -1.7 -1.9 tRNA-Trp (NCBI) compare
b3794 rffM -1.7 -6.6 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b2512 yfgL -1.6 -4.5 protein assembly complex, lipoprotein component (NCBI) compare
b3630 rfaP -1.6 -4.5 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b3785 wzzE -1.6 -9.4 putative transport protein (VIMSS) compare
b3786 rffE -1.6 -10.1 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b4481 rffT -1.6 -5.7 4-alpha-L-fucosyltransferase (NCBI) compare
b1323 tyrR -1.6 -4.4 DNA-binding transcriptional dual regulator, tyrosine-binding (NCBI) compare
b2236 yfaE -1.6 -2.3 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b3787 rffD -1.6 -8.9 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b4550 yhdL -1.6 -4.4 hypothetical protein (NCBI) compare
b3627 rfaI -1.6 -5.2 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b4570 lomR -1.6 -2.0 no description compare
b2064 asmA -1.6 -4.3 predicted assembly protein (NCBI) compare
b0843 ybjH -1.6 -2.6 hypothetical protein (NCBI) compare
b3619 rfaD -1.6 -1.5 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b1685 ydiH -1.5 -1.3 predicted protein (RefSeq) compare
b2829 ptsP -1.5 -8.2 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b4414 tff -1.5 -2.1 unknown RNA (NCBI) compare
b1413 hrpA -1.5 -6.7 ATP-dependent helicase (RefSeq) conserved
b1518 lsrG -1.5 -1.9 autoinducer-2 (AI-2) modifying protein LsrG (NCBI) compare
b3609 secB -1.5 -3.5 export protein SecB (NCBI) compare
b0014 dnaK -1.5 -1.3 molecular chaperone DnaK (NCBI) compare
b1278 pgpB -1.5 -2.1 phosphatidylglycerophosphatase B (NCBI) compare
b2579 yfiD -1.4 -1.8 pyruvate formate lyase subunit (NCBI) compare
b4701 sokX -1.4 -0.9 sok-related sRNA, function unknown (RefSeq) compare
b1863 ruvC -1.4 -1.0 Holliday junction resolvase (NCBI) compare
b1482 osmC -1.4 -2.0 osmotically inducible, stress-inducible membrane protein (NCBI) compare
b0053 surA -1.4 -1.6 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b3791 rffA -1.4 -5.8 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b2617 smpA -1.4 -2.3 small membrane protein A (VIMSS) compare
b2553 glnB -1.4 -3.2 regulatory protein P-II for glutamine synthetase (NCBI) compare
b3931 hslU -1.3 -7.8 ATP-dependent protease ATP-binding subunit (NCBI) compare
b3387 dam -1.3 -7.3 DNA adenine methylase (NCBI) compare
b1861 ruvA -1.3 -0.9 Holliday junction DNA helicase motor protein (NCBI) compare
b3764 yifE -1.3 -3.7 hypothetical protein (NCBI) compare
b2377 yfdY -1.3 -2.1 predicted inner membrane protein (NCBI) compare
b3961 oxyR -1.3 -7.4 DNA-binding transcriptional dual regulator (NCBI) compare
b0247 ykfG -1.3 -1.3 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b0001 thrL -1.3 -2.0 thr operon leader peptide (NCBI) compare
b1540 ydfH -1.3 -1.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b4679 yohP -1.3 -2.0 expressed protein (RefSeq) compare
b0877 ybjX -1.3 -4.4 hypothetical protein (NCBI) compare
b0925 ycbB -1.3 -5.4 predicted carboxypeptidase (NCBI) compare
b0015 dnaJ -1.3 -5.7 chaperone Hsp40, co-chaperone with DnaK (NCBI) compare
b0746 valZ -1.2 -1.4 tRNA-Val (NCBI) compare
b4596 yciZ -1.2 -1.5 hypothetical protein (RefSeq) compare
b3229 sspA -1.2 -1.2 stringent starvation protein A (NCBI) compare
b0632 dacA -1.2 -5.3 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b0738 tolR -1.2 -1.4 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b1954 dsrA -1.2 -2.0 regulatory antisense RNA (NCBI) compare
b1831 proQ -1.2 -2.7 putative solute/DNA competence effector (NCBI) compare
b2258 b2258 -1.2 -1.2 putative transport/receptor protein (VIMSS) compare
b0122 yacC -1.2 -3.5 orf, hypothetical protein (VIMSS) compare
b1104 ycfL -1.2 -1.5 hypothetical protein (NCBI) compare
b0741 pal -1.2 -1.7 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b3082 ygjM -1.2 -0.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b1103 ycfF -1.2 -1.1 orf, hypothetical protein (VIMSS) compare
b0222 lpcA -1.2 -1.8 phosphoheptose isomerase (NCBI) compare
b4685 yrbN -1.2 -1.7 expressed protein (RefSeq) compare
b2080 b2080 -1.1 -1.6 orf, hypothetical protein (VIMSS) compare
b0677 nagA -1.1 -3.6 N-acetylglucosamine-6-phosphate deacetylase (NCBI) compare
b1348 lar -1.1 -1.2 Rac prophage; restriction alleviation protein (NCBI) compare
b3396 mrcA -1.1 -9.2 peptidoglycan synthetase; penicillin-binding protein 1A (VIMSS) compare
b3644 yicC -1.1 -4.2 hypothetical protein (NCBI) compare
b2807 ygdD -1.1 -3.2 conserved inner membrane protein (NCBI) compare
b2809 b2809 -1.1 -2.4 orf, hypothetical protein (VIMSS) compare
b1567 intK -1.1 -1.1 Qin prophage; predicted protein (RefSeq) compare
b1105 ycfM -1.1 -2.9 predicted outer membrane lipoprotein (NCBI) compare
b4062 soxS -1.1 -3.3 DNA-binding transcriptional dual regulator (NCBI) compare
b0836 yliH -1.1 -2.0 hypothetical protein (NCBI) compare
b2144 sanA -1.1 -5.1 hypothetical protein (NCBI) compare
b4529 ydbJ -1.1 -0.9 hypothetical protein (NCBI) compare
b3147 yraM -1.1 -7.0 hypothetical protein (NCBI) compare
b1176 minC -1.1 -2.0 septum formation inhibitor (NCBI) compare
b3026 qseC -1.1 -0.7 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b0825 mipB -1.0 -3.1 putative transaldolase (VIMSS) compare
b2209 eco -1.0 -1.7 ecotin precursor (NCBI) compare
b2038 rfbC -1.0 -3.8 dTDP-4-deoxyrhamnose-3,5-epimerase (NCBI) compare
b0989 cspH -1.0 -1.2 stress protein, member of the CspA-family (NCBI) compare
b4480 hdfR -1.0 -3.7 transcriptional regulator HdfR (NCBI) compare
b2494 yfgC -1.0 -4.5 predicted peptidase (NCBI) compare
b3932 hslV -1.0 -6.2 ATP-dependent protease peptidase subunit (NCBI) compare
b4151 frdD -1.0 -2.4 fumarate reductase subunit D (NCBI) compare
b4042 dgkA -1.0 -0.7 diacylglycerol kinase (NCBI) compare
b2814 metZ -1.0 -1.2 tRNA-Met (NCBI) compare
b2893 dsbC -1.0 -4.0 protein disulfide isomerase II (NCBI) compare
b1126 potA -1.0 -3.9 putrescine/spermidine ABC transporter ATPase protein (NCBI) compare
b4503 yafF -1.0 -0.6 no description compare
b2646 ypjF -1.0 -1.6 CP4-57 prophage; toxin of the YpjF-YfjZ toxin-antitoxin system (NCBI) compare
b0108 ppdD -1.0 -3.1 predicted major pilin subunit (NCBI) compare
b1344 ydaO -1.0 -1.9 predicted C32 tRNA thiolase (NCBI) compare
b4202 rpsR -1.0 -0.7 30S ribosomal protein S18 (NCBI) compare
b2910 zapA -1.0 -3.1 protein that localizes to the cytokinetic ring (NCBI) compare
b1124 potC -1.0 -3.1 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b0740 tolB -1.0 -1.7 translocation protein TolB precursor (NCBI) compare
b1289 ycjD -1.0 -2.3 hypothetical protein (NCBI) compare
b2558 mltF -1.0 -6.0 predicted periplasmic binding protein/transglycosylase (RefSeq) compare
b1860 ruvB -1.0 -0.6 Holliday junction DNA helicase B (NCBI) compare
b0149 mrcB -1.0 -6.4 penicillin-binding protein 1b (NCBI) compare
b2282 nuoH -1.0 -2.0 NADH dehydrogenase subunit H (NCBI) compare
b1164 ycgZ -1.0 -0.6 hypothetical protein (NCBI) compare
b1175 minD -1.0 -2.3 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b3844 fre -1.0 -3.4 NAD(P)H-flavin reductase (NCBI) compare
b1871 yecP -1.0 -2.1 predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI) compare
b2444 yffM -1.0 -1.3 CPZ-55 prophage; predicted protein (NCBI) compare
b2344 fadL -1.0 -4.8 long-chain fatty acid outer membrane transporter (RefSeq) compare
b4608 ryfC -1.0 -2.0 Novel sRNA, function unknown (RefSeq) compare
b0736 ybgC -1.0 -2.4 predicted acyl-CoA thioesterase (NCBI) compare
b3652 recG -1.0 -4.7 ATP-dependent DNA helicase (NCBI) compare
b1125 potB -0.9 -2.4 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b0784 moaD -0.9 -1.9 molybdopterin synthase, small subunit (NCBI) compare
b1671 ydhX -0.9 -1.8 predicted 4Fe-4S ferridoxin-type protein (RefSeq) compare
b3933 ftsN -0.9 -5.4 essential cell division protein (NCBI) compare
b3658 selC -0.9 -2.0 tRNA-Sec (NCBI) compare
b1523 yneG -0.9 -1.7 hypothetical protein (NCBI) compare
b1562 hokD -0.9 -1.6 Qin prophage; small toxic polypeptide (NCBI) compare
b2237 inaA -0.9 -2.1 hypothetical protein (NCBI) compare
b1790 yeaM -0.9 -1.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b4096 phnL -0.9 -2.5 carbon-phosphorus lyase complex subunit (NCBI) compare
b2181 yejG -0.9 -2.5 hypothetical protein (NCBI) compare
b4396 rob -0.9 -5.5 DNA-binding transcriptional activator (NCBI) compare
b4676 yoaK -0.9 -0.6 expressed protein (RefSeq) compare
b4402 yjjY -0.9 -0.6 hypothetical protein (NCBI) compare
b0737 tolQ -0.9 -1.0 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b1647 ydhF -0.9 -2.7 predicted oxidoreductase (NCBI) compare
b3085 ygjP -0.9 -3.1 orf, hypothetical protein (VIMSS) compare
b4045 yjbJ -0.9 -1.3 predicted stress response protein (NCBI) compare
b2906 visC -0.9 -3.1 hypothetical protein (NCBI) compare
b3206 npr -0.9 -1.9 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b0739 tolA -0.9 -1.0 cell envelope integrity inner membrane protein TolA (NCBI) compare
b1524 yneH -0.9 -0.7 predicted glutaminase (NCBI) compare
b3807 cyaY -0.9 -4.1 frataxin-like protein (NCBI) compare
b1353 sieB -0.9 -2.5 phage superinfection exclusion protein (VIMSS) compare
b3207 yrbL -0.8 -3.2 hypothetical protein (NCBI) compare
b1236 galU -0.8 -1.7 glucose-1-phosphate uridylyltransferase (NCBI) compare
b4046 yjbK -0.8 -3.3 putative regulator (VIMSS) compare
b2572 rseA -0.8 -2.8 anti-sigma factor (NCBI) compare
b1349 recT -0.8 -3.1 Rac prophage; recombination and repair protein (NCBI) compare
b0542 renD -0.8 -1.4 orf, hypothetical protein (VIMSS) compare
b2040 rfbD -0.8 -4.5 dTDP-4-dehydrorhamnose reductase subunit, NAD(P)-binding, of dTDP-L-rhamnose synthase (NCBI) compare
b0819 ybiS -0.8 -4.0 hypothetical protein (NCBI) compare
b1302 puuE -0.8 -2.1 GABA aminotransferase, PLP-dependent (NCBI) compare
b1286 rnb -0.8 -1.1 exoribonuclease II (NCBI) compare
b2027 wzzB -0.8 -2.8 regulator of length of O-antigen component of lipopolysaccharide chains (VIMSS) compare
b3545 proK -0.8 -1.5 tRNA-Pro (NCBI) compare
b2314 dedD -0.8 -2.0 putative lipoprotein (VIMSS) compare
b1438 ydcQ -0.8 -1.7 predicted DNA-binding transcriptional regulator (RefSeq) compare
b1834 yebT -0.8 -4.1 orf, hypothetical protein (VIMSS) compare
b0049 apaH -0.8 -1.1 diadenosinetetraphosphatase (NCBI) compare
b4406 yaeP -0.8 -1.5 hypothetical protein (NCBI) compare
b0549 ybcO -0.8 -1.4 DLP12 prophage; predicted protein (NCBI) compare
b3628 rfaB -0.8 -3.4 UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase (RefSeq) compare
b1153 ymfQ -0.8 -1.3 e14 prophage; conserved protein (NCBI) compare
b0013 yaaI -0.8 -2.8 hypothetical protein (NCBI) compare
b4413 sokC -0.8 -1.1 regulatory antisense RNA (NCBI) compare


Specific Phenotypes

For 6 genes in this experiment

For stress Doxycycline hyclate in Escherichia coli BW25113

For stress Doxycycline hyclate across organisms