Experiment set8IT012 for Escherichia coli BW25113

LB Aerobic with Chlorhexidine diacetate salt hydrate 0.000000625 mM

Group: stress
Media: LB + Chlorhexidine diacetate salt hydrate (6.25E-04 mM)
Culturing: Keio_ML9a, 96 deep-well microplate; Multitron, Aerobic, at 37 (C), shaken=750 rpm
By: Hans_Hualan on 7/20/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 43 genes in this experiment

For stress Chlorhexidine diacetate salt hydrate in Escherichia coli BW25113

For stress Chlorhexidine diacetate salt hydrate across organisms

SEED Subsystems

Subsystem	#Specific
Formate hydrogenase	3
Formate dehydrogenase	2
Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes	2
Proteasome bacterial	2
Proteolysis in bacteria, ATP-dependent	2
ABC transporter oligopeptide (TC 3.A.1.5.1)	1
Beta-Glucoside Metabolism	1
Capsular heptose biosynthesis	1
Deoxyribose and Deoxynucleoside Catabolism	1
Glycine and Serine Utilization	1
LOS core oligosaccharide biosynthesis	1
Oxidative stress	1
Purine conversions	1
Pyruvate Alanine Serine Interconversions	1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate	1
Selenocysteine metabolism	1
Threonine anaerobic catabolism gene cluster	1
Universal stress protein family	1
tRNA processing	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Porphyrin and chlorophyll metabolism
• Phenylpropanoid biosynthesis
• Pentose phosphate pathway
• Pentose and glucuronate interconversions
• Galactose metabolism
• Ubiquinone and menaquinone biosynthesis
• Pyrimidine metabolism
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Cyanoamino acid metabolism
• Starch and sucrose metabolism
• Glycerolipid metabolism
• Inositol phosphate metabolism
• Naphthalene and anthracene degradation
• Glyoxylate and dicarboxylate metabolism
• Methane metabolism
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Drug metabolism - other enzymes
• Biosynthesis of ansamycins
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
formate oxidation to CO2	1	1	1
neolinustatin bioactivation	3	2	2
siroheme biosynthesis	4	4	2
pseudouridine degradation	2	2	1
guanine and guanosine salvage I	2	2	1
xanthine and xanthosine salvage	2	2	1
formate to trimethylamine N-oxide electron transfer	2	2	1
guanine and guanosine salvage II	2	2	1
formate to dimethyl sulfoxide electron transfer	2	2	1
nitrate reduction III (dissimilatory)	2	2	1
linustatin bioactivation	4	2	2
lotaustralin degradation	2	1	1
linamarin degradation	2	1	1
superpathway of guanine and guanosine salvage	3	3	1
2-deoxy-α-D-ribose 1-phosphate degradation	3	3	1
adenine salvage	3	3	1
formate to nitrite electron transfer	3	2	1
cellulose degradation II (fungi)	3	2	1
factor 430 biosynthesis	7	3	2
adenine and adenosine salvage III	4	4	1
oxalate degradation VI	4	2	1
GDP-D-glycero-α-D-manno-heptose biosynthesis	4	1	1
ADP-L-glycero-β-D-manno-heptose biosynthesis	5	5	1
oxalate degradation III	5	2	1
coumarin biosynthesis (via 2-coumarate)	5	2	1
purine ribonucleosides degradation	6	6	1
superpathway of pyrimidine deoxyribonucleosides degradation	6	6	1
α-tomatine degradation	6	1	1
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation)	13	2	2
superpathway of purine deoxyribonucleosides degradation	7	7	1
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion)	15	3	2
superpathway of C1 compounds oxidation to CO2	12	4	1
superpathway of purine nucleotide salvage	14	13	1
firefly bioluminescence	14	2	1
purine nucleobases degradation I (anaerobic)	15	6	1
adenosylcobalamin biosynthesis II (aerobic)	33	17	2
adenosylcobalamin biosynthesis I (anaerobic)	36	16	2
purine nucleobases degradation II (anaerobic)	24	17	1