Experiment set8IT012 for Escherichia coli BW25113

LB Aerobic with Chlorhexidine diacetate salt hydrate 0.000000625 mM

Group: stress
Media: LB + Chlorhexidine diacetate salt hydrate (6.25E-04 mM)
Culturing: Keio_ML9a, 96 deep-well microplate; Multitron, Aerobic, at 37 (C), shaken=750 rpm
By: Hans_Hualan on 7/20/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 42 genes in this experiment

For stress Chlorhexidine diacetate salt hydrate in Escherichia coli BW25113

For stress Chlorhexidine diacetate salt hydrate across organisms

SEED Subsystems

Subsystem	#Specific
Formate hydrogenase	3
Formate dehydrogenase	2
Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes	2
Proteasome bacterial	2
Proteolysis in bacteria, ATP-dependent	2
Selenocysteine metabolism	2
ABC transporter oligopeptide (TC 3.A.1.5.1)	1
Beta-Glucoside Metabolism	1
Capsular heptose biosynthesis	1
Deoxyribose and Deoxynucleoside Catabolism	1
Glycine and Serine Utilization	1
LOS core oligosaccharide biosynthesis	1
Oxidative stress	1
Pyruvate Alanine Serine Interconversions	1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate	1
Threonine anaerobic catabolism gene cluster	1
Universal stress protein family	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Porphyrin and chlorophyll metabolism
• Phenylpropanoid biosynthesis
• Pentose phosphate pathway
• Pentose and glucuronate interconversions
• Galactose metabolism
• Ubiquinone and menaquinone biosynthesis
• Purine metabolism
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Selenoamino acid metabolism
• Cyanoamino acid metabolism
• Starch and sucrose metabolism
• Glycerolipid metabolism
• Inositol phosphate metabolism
• Naphthalene and anthracene degradation
• Glyoxylate and dicarboxylate metabolism
• Methane metabolism
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Aminoacyl-tRNA biosynthesis
• Insect hormone biosynthesis
• Biosynthesis of ansamycins
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
formate oxidation to CO2	1	1	1
neolinustatin bioactivation	3	2	2
siroheme biosynthesis	4	4	2
formate to trimethylamine N-oxide electron transfer	2	2	1
formate to dimethyl sulfoxide electron transfer	2	2	1
nitrate reduction III (dissimilatory)	2	2	1
linustatin bioactivation	4	2	2
linamarin degradation	2	1	1
lotaustralin degradation	2	1	1
2-deoxy-α-D-ribose 1-phosphate degradation	3	3	1
L-selenocysteine biosynthesis I (bacteria)	3	3	1
cellulose degradation II (fungi)	3	2	1
formate to nitrite electron transfer	3	2	1
factor 430 biosynthesis	7	3	2
oxalate degradation VI	4	2	1
GDP-D-glycero-α-D-manno-heptose biosynthesis	4	1	1
ADP-L-glycero-β-D-manno-heptose biosynthesis	5	5	1
coumarin biosynthesis (via 2-coumarate)	5	2	1
oxalate degradation III	5	2	1
purine ribonucleosides degradation	6	6	1
superpathway of pyrimidine deoxyribonucleosides degradation	6	6	1
α-tomatine degradation	6	1	1
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation)	13	2	2
superpathway of purine deoxyribonucleosides degradation	7	7	1
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion)	15	3	2
superpathway of C1 compounds oxidation to CO2	12	4	1
firefly bioluminescence	14	2	1
purine nucleobases degradation I (anaerobic)	15	6	1
adenosylcobalamin biosynthesis II (aerobic)	33	17	2
adenosylcobalamin biosynthesis I (anaerobic)	36	16	2
purine nucleobases degradation II (anaerobic)	24	17	1