Experiment set81S254 for Escherichia coli BW25113

GL_40

Group: toxin
Media: LB + double orbital, continuous, 205cpm (Gliotoxin 40) + ug/mL
Culturing: Keio_ML9a, 48 well microplate, Liquid, at 37 (C), shaken=Aerobic, (Toxin)
By: Phoebe on 5/17/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 11 genes in this experiment

For toxin double orbital, continuous, 205cpm in Escherichia coli BW25113

For toxin double orbital, continuous, 205cpm across organisms

SEED Subsystems

Subsystem	#Specific
Pentose phosphate pathway	1
Ribonucleotide reduction	1
Staphylococcal phi-Mu50B-like prophages	1
Ton and Tol transport systems	1
Transport of Manganese	1
Universal stress protein family	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pyrimidine metabolism
• Glutathione metabolism
• Pentose phosphate pathway
• Purine metabolism
• Selenoamino acid metabolism
• Thiamine metabolism
• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-cysteine degradation IV	1	1	1
L-alanine biosynthesis III	1	1	1
adenosine deoxyribonucleotides de novo biosynthesis II	4	4	2
guanosine deoxyribonucleotides de novo biosynthesis II	4	4	2
di-trans,poly-cis-undecaprenyl phosphate biosynthesis	2	2	1
adenosine deoxyribonucleotides de novo biosynthesis I	2	2	1
pseudouridine degradation	2	2	1
guanosine deoxyribonucleotides de novo biosynthesis I	2	2	1
cytidylyl molybdenum cofactor sulfurylation	2	1	1
pentose phosphate pathway (oxidative branch) I	3	3	1
bis(guanylyl molybdopterin) cofactor sulfurylation	3	1	1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli)	14	14	4
superpathway of adenosine nucleotides de novo biosynthesis II	7	7	2
thiazole component of thiamine diphosphate biosynthesis II	7	5	2
superpathway of guanosine nucleotides de novo biosynthesis II	8	8	2
superpathway of L-alanine biosynthesis	4	4	1
tRNA-uridine 2-thiolation (yeast mitochondria)	4	1	1
tRNA-uridine 2-thiolation (mammalian mitochondria)	4	1	1
pyrimidine deoxyribonucleotides de novo biosynthesis I	9	9	2
pyrimidine deoxyribonucleotides de novo biosynthesis III	9	8	2
polyisoprenoid biosynthesis (E. coli)	5	5	1
superpathway of adenosine nucleotides de novo biosynthesis I	5	5	1
[2Fe-2S] iron-sulfur cluster biosynthesis	10	4	2
tRNA-uridine 2-thiolation (thermophilic bacteria)	5	1	1
superpathway of thiamine diphosphate biosynthesis II	11	9	2
thiazole component of thiamine diphosphate biosynthesis I	6	6	1
superpathway of guanosine nucleotides de novo biosynthesis I	6	6	1
molybdopterin biosynthesis	6	6	1
superpathway of purine nucleotides de novo biosynthesis II	26	26	4
pyrimidine deoxyribonucleotides de novo biosynthesis IV	7	6	1
pentose phosphate pathway	8	8	1
pyrimidine deoxyribonucleotides biosynthesis from CTP	8	6	1
tRNA-uridine 2-thiolation (cytoplasmic)	8	1	1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis	18	18	2
superpathway of thiamine diphosphate biosynthesis I	10	10	1
superpathway of purine nucleotides de novo biosynthesis I	21	21	2
tRNA-uridine 2-thiolation and selenation (bacteria)	11	7	1
superpathway of histidine, purine, and pyrimidine biosynthesis	46	46	4
superpathway of purine nucleotide salvage	14	13	1
superpathway of glucose and xylose degradation	17	17	1