Experiment set7S80 for Escherichia coli BL21
CM1
Group: phageMedia: LB_plus_SM_buffer + MOI 10
Culturing: BL21_ML4, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 10/18/23
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 14 genes in this experiment
For phage MOI 10 in Escherichia coli BL21
For phage MOI 10 across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Capsular Polysaccharide (CPS) of Campylobacter | 3 |
| Rhamnose containing glycans | 2 |
| D-ribose utilization | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-threonine degradation II | 2 | 2 | 1 |
| 8-amino-7-oxononanoate biosynthesis II | 2 | 1 | 1 |
| 8-amino-7-oxononanoate biosynthesis III | 2 | 1 | 1 |
| CMP-3-deoxy-D-manno-octulosonate biosynthesis | 5 | 5 | 1 |
| 8-amino-7-oxononanoate biosynthesis IV | 5 | 4 | 1 |
| biotin biosynthesis II | 6 | 4 | 1 |
| CMP-8-amino-3,8-dideoxy-D-manno-octulosonate biosynthesis | 7 | 5 | 1 |
| 8-amino-7-oxononanoate biosynthesis I | 11 | 11 | 1 |
| biotin biosynthesis I | 15 | 15 | 1 |
| superpathway of (Kdo)2-lipid A biosynthesis | 17 | 17 | 1 |
| superpathway of L-threonine metabolism | 18 | 16 | 1 |
| superpathway of Kdo2-lipid A biosynthesis | 25 | 21 | 1 |