Experiment set7S654 for Pseudomonas segetis P6

Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days

Group: in planta
Media: + Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
Culturing: Pseudo_segetis_P6_ML4b, pot, at 34 (C), (Solid)
By: Marta Torres on 15-Jun-24

Specific Phenotypes

For 7 genes in this experiment

For in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days in Pseudomonas segetis P6

For in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days across organisms

SEED Subsystems

Subsystem	#Specific
Pyruvate metabolism I: anaplerotic reactions, PEP	2
ABC transporter dipeptide (TC 3.A.1.5.2)	1
Fermentations: Mixed acid	1
Glycolysis and Gluconeogenesis	1
Glycolysis and Gluconeogenesis, including Archaeal enzymes	1
Lipid A modifications	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)
• Carbon fixation in photosynthetic organisms

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
CO2 fixation into oxaloacetate (anaplerotic)	2	2	1
NAD salvage pathway II (PNC IV cycle)	5	4	1
C4 photosynthetic carbon assimilation cycle, NADP-ME type	7	4	1
partial TCA cycle (obligate autotrophs)	8	8	1
nitrogen remobilization from senescing leaves	8	6	1
gluconeogenesis II (Methanobacterium thermoautotrophicum)	18	9	2
glycolysis V (Pyrococcus)	10	7	1
glycolysis II (from fructose 6-phosphate)	11	9	1
C4 photosynthetic carbon assimilation cycle, NAD-ME type	11	6	1
gluconeogenesis I	13	11	1
glycolysis I (from glucose 6-phosphate)	13	10	1
formaldehyde assimilation I (serine pathway)	13	6	1
C4 photosynthetic carbon assimilation cycle, PEPCK type	14	9	1
mixed acid fermentation	16	10	1
superpathway of glycolysis and the Entner-Doudoroff pathway	17	14	1
superpathway of hexitol degradation (bacteria)	18	10	1
superpathway of anaerobic sucrose degradation	19	15	1
hexitol fermentation to lactate, formate, ethanol and acetate	19	13	1
superpathway of N-acetylneuraminate degradation	22	12	1
ethene biosynthesis V (engineered)	25	18	1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass	26	21	1
Methanobacterium thermoautotrophicum biosynthetic metabolism	56	20	2