Experiment set7S593 for Pseudomonas aeruginosa MRSN321

Ab20_d

Group: phage
Media: LB
Culturing: Paeruginosa_MRSN321_ML2, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: DP on 9/4/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 23 genes in this experiment

SEED Subsystems

Subsystem	#Specific
Alanine biosynthesis	1
D-ribose utilization	1
KDO2-Lipid A biosynthesis	1
Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway	1
Lactose and Galactose Uptake and Utilization	1
N-linked Glycosylation in Bacteria	1
Peptidoglycan Biosynthesis	1
Phosphate metabolism	1
Rhamnose containing glycans	1
SigmaB stress responce regulation	1
Ton and Tol transport systems	1
Transport of Zinc	1
Type IV pilus	1
linker unit-arabinogalactan synthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Galactose metabolism
• Fatty acid biosynthesis
• Ubiquinone and menaquinone biosynthesis
• Tryptophan metabolism
• Nucleotide sugars metabolism
• alpha-Linolenic acid metabolism
• Benzoate degradation via CoA ligation
• Thiamine metabolism
• Limonene and pinene degradation
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
UDP-N-acetyl-D-galactosamine biosynthesis I	1	1	1
L-cysteine degradation IV	1	1	1
UDP-α-D-galactose biosynthesis	1	1	1
L-alanine biosynthesis III	1	1	1
UDP-α-D-galactofuranose biosynthesis	2	1	1
cytidylyl molybdenum cofactor sulfurylation	2	1	1
D-galactose detoxification	3	1	1
bis(guanylyl molybdopterin) cofactor sulfurylation	3	1	1
thiazole component of thiamine diphosphate biosynthesis II	7	5	2
superpathway of L-alanine biosynthesis	4	4	1
tRNA-uridine 2-thiolation (mammalian mitochondria)	4	1	1
tRNA-uridine 2-thiolation (yeast mitochondria)	4	1	1
[2Fe-2S] iron-sulfur cluster biosynthesis	10	4	2
D-galactose degradation I (Leloir pathway)	5	2	1
tRNA-uridine 2-thiolation (thermophilic bacteria)	5	1	1
superpathway of thiamine diphosphate biosynthesis II	11	9	2
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis	6	4	1
thiazole component of thiamine diphosphate biosynthesis I	6	4	1
molybdopterin biosynthesis	6	4	1
alginate biosynthesis II (bacterial)	7	6	1
UDP-N-acetyl-D-galactosamine biosynthesis II	7	5	1
stachyose degradation	7	2	1
tRNA-uridine 2-thiolation (cytoplasmic)	8	1	1
UDP-sugars interconversion	9	2	1
teichuronic acid biosynthesis (B. subtilis 168)	9	2	1
superpathway of thiamine diphosphate biosynthesis I	10	8	1
colanic acid building blocks biosynthesis	11	9	1
tRNA-uridine 2-thiolation and selenation (bacteria)	11	5	1
mycolyl-arabinogalactan-peptidoglycan complex biosynthesis	18	1	1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis	33	12	1