Experiment set7S214 for Escherichia coli BL21
EV152
Group: phageMedia: LB_plus_SM_buffer + MOI 10
Culturing: BL21_ML4, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 1/29/24
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 9 genes in this experiment
For phage MOI 10 in Escherichia coli BL21
For phage MOI 10 across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Formate hydrogenase | 3 |
| Formate dehydrogenase | 2 |
| CytR regulation | 1 |
| LOS core oligosaccharide biosynthesis | 1 |
| Lipid A modifications | 1 |
| Molybdenum cofactor biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| formate oxidation to CO2 | 1 | 1 | 1 |
| formate to dimethyl sulfoxide electron transfer | 2 | 2 | 1 |
| nitrate reduction III (dissimilatory) | 2 | 2 | 1 |
| formate to trimethylamine N-oxide electron transfer | 2 | 2 | 1 |
| formate to nitrite electron transfer | 3 | 2 | 1 |
| oxalate degradation VI | 4 | 2 | 1 |
| oxalate degradation III | 5 | 2 | 1 |
| molybdopterin biosynthesis | 6 | 6 | 1 |
| superpathway of C1 compounds oxidation to CO2 | 12 | 4 | 1 |
| purine nucleobases degradation I (anaerobic) | 15 | 6 | 1 |
| purine nucleobases degradation II (anaerobic) | 24 | 17 | 1 |