Experiment set7S187 for Escherichia coli BL21
Bas30
Group: phageMedia: LB_plus_SM_buffer + MOI 10
Culturing: BL21_ML4, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 1/19/24
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 1 genes in this experiment
For phage MOI 10 in Escherichia coli BL21
For phage MOI 10 across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Serine-glyoxylate cycle | 1 |
| TCA Cycle | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Propanoate metabolism
- Biosynthesis of plant hormones
- Citrate cycle (TCA cycle)
- Geraniol degradation
- Tyrosine metabolism
- alpha-Linolenic acid metabolism
- Benzoate degradation via CoA ligation
- Ethylbenzene degradation
- C5-Branched dibasic acid metabolism
- Reductive carboxylate cycle (CO2 fixation)
- Limonene and pinene degradation
- Caprolactam degradation
- Alkaloid biosynthesis II
- Biosynthesis of phenylpropanoids
- Biosynthesis of terpenoids and steroids
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from terpenoid and polyketide
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| pyruvate fermentation to acetate V | 3 | 2 | 1 |
| pyruvate fermentation to acetate VI | 3 | 2 | 1 |
| adipate degradation | 5 | 4 | 1 |
| adipate biosynthesis | 5 | 3 | 1 |
| TCA cycle VIII (Chlamydia) | 6 | 5 | 1 |
| incomplete reductive TCA cycle | 7 | 5 | 1 |
| TCA cycle V (2-oxoglutarate synthase) | 9 | 7 | 1 |
| TCA cycle II (plants and fungi) | 9 | 7 | 1 |
| TCA cycle I (prokaryotic) | 10 | 9 | 1 |
| TCA cycle III (animals) | 10 | 8 | 1 |
| anaerobic energy metabolism (invertebrates, mitochondrial) | 10 | 7 | 1 |
| reductive TCA cycle I | 11 | 8 | 1 |
| superpathway of glyoxylate bypass and TCA | 12 | 11 | 1 |
| reductive TCA cycle II | 12 | 8 | 1 |
| superpathway of anaerobic energy metabolism (invertebrates) | 17 | 12 | 1 |
| methylaspartate cycle | 19 | 9 | 1 |
| superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle | 22 | 18 | 1 |
| superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass | 26 | 25 | 1 |
| Methanobacterium thermoautotrophicum biosynthetic metabolism | 56 | 22 | 1 |